采用高效液相色谱-串联质谱法检测鲫鱼组织3-甲基喹(噁)啉-2-羧酸残留量

将待测鱼肉和鱼皮匀浆样品经碱水解、液-液萃取后,采用HPLC-TMS方法对其中的3-甲基喹(噁)啉-2-羧酸(MQCA)残留量进行了检测.结果,此方法的最低检测限为1.16μg/kg,市售鲫鱼样品中MQCA的含量低于检测限.结果表明,HPLC-TMC灵敏,易操作,能满足鱼组织内MQCA残留量检测的要求.     

犬瘟热病毒RT-nested PCR检测方法的建立与应用

根据犬瘟热病毒(CDV)弱毒株Onderstepoort的核衣壳蛋白(NP)基因序列设计了套式引物,建立了RT-nested PCR检测方法。特异性试验表明,该法可以特异扩增出CDV NP基因片段,但从RNA病毒狂犬病病毒(RV)、犬副流感病毒(CPIV)、犬冠状病毒(CCV),DNA病毒犬细小病毒(CPV)、犬腺病毒(CAV)及正常Vero细胞中均不能扩增出条带。敏感性试验表明,RT-PCR可以扩增10-6稀释度的病毒RNA,而建立的RT-nested PCR可以扩增到10-9稀释度的病毒RNA,后者的敏感性明显高于前者。用RT-nested PCR法检测了2006年我国东北地区临床表现犬瘟热症状的病例19例,检出率达90%,明显高于RT-PCR的检出率(45%)。部分病例扩增片段的测序结果表明,CDV NP基因出现个别碱基变异。研究结果表明,建立的犬瘟热病毒RT-nested PCR方法敏感、特异,适用于动物犬瘟热的快速诊断。     

妨害传染病防治罪的几个构成要件问题

妨害传染病防治罪的客体应当表述为"国家对传染病防治的管理制度";其客观要件"引起甲类传染病传播或者有传播严重危险"包括实害犯与危险犯两种形态;其主观要件应为过失,故意则不能构成。     

在新的历史起点上推动人民政协制度更加成熟定型

国家政治制度成熟定型必须具备系统完备、相对稳定、科学有效和广泛认同四个基本要件,人民政协制度更加成熟定型既要遵从上述要件,又要考虑政协性质特点,做到理论和政治上成熟,有鲜明的政治定性;职责和功能上成熟,有稳定的职能定位;制度机制成熟,有完备的工作定规。70年来人民政协制度化建设稳步推进,各项制度体系正在加速形成,新时代人民政协要更好地发挥在国家治理中专门协商机构作用,必须加快协商制度创新和完善,在新的历史起点上推动人民政协制度更加成熟定型。     

Use of Eucalyptus DNA profiling in a case of illegal logging

Eucalyptus is grown world-wide for paper pulp, solid wood, and other industries. Theft or illegal cutting of the trees causes hardship to owners of plantations and countries whose economies rely on the sale and export of eucalyptus products. Unfortunately, many of these crimes go unpunished due to lack of forensic evidence.Over 1200 short tandem repeat (STR) markers have been identified in the genomes of genus Eucalyptus and related species. However, their importance and utility in aiding forensic investigations of wood theft have not been explored. This study evaluated nine STRs for diversity and applied them to a case involving suspected wood theft.As expected, three dinucleotide STR markers showed greater variability but resulted in harder to interpret profiles. Four STR tetranucleotide markers evaluated in this study were found to contain additional repeat structures (dinucleotide or trinucleotide) that enhanced their variability but resulted in profiles with peaks at multiple stutter positions and heterozygote peak imbalance. The most promising STR markers were EGM37 and EMBRA 1374. Though less variable, they yielded robust and reproducible DNA profiles.All nine STR markers were applied to a case involving suspected wood theft. Samples were collected from seized wood and from remaining stumps in a plantation. No DNA match was found, thus eliminating the evidence samples as having originated from the forest. Dendrochronology analysis also resulted in an exclusion. This case study represents the first report using STR markers in any eucalyptus species to provide DNA evidence in a case of suspected wood theft.     

The Curious Incident of Mr Cameron and the United Kingdom Defence Budget: A New Legacy?

During 2015 Prime Minister Cameron found himself under intense domestic and international pressure over his apparent reluctance to maintain United Kingdom defence spending at the NATO target level of 2 per cent of GDP. Most commentators attributed this reluctance to the inevitability of defence cuts if the government wished to meet its deficit reduction targets. However, the aftermath of the general election saw a sudden decision to maintain UK defence spending at the NATO target level. This u‐turn is one of the more curious episodes in recent British defence policy. In this article we explore the reasons why, at a time of continuing cuts and austerity measures and against all the political signals, a decision was made to meet the 2 per cent target, and what this means for the UK's defence policy. In doing so, we analyse why most commentators assumed that defence cuts were inevitable, the domestic and international factors that explain the government's apparent u‐turn and what this revised defence budget settlement meant for the new 2015 National Security Strategy and Strategic Defence and Security Review.     

道德入律之我见

本文就重大 (特殊 )的道德义务能否作为不作为犯罪中的作为义务来源展开了讨论。文中介绍了欧美各国的现行做法 ,同时在法理上和政策上论证了将道德义务入律的可行性。     

法院司法问责若干问题研究

党的十八届四中全会指出,完善主审法官、合议庭办案责任制,落实谁办案谁负责。这就提出了如何进行司法问责的问题。而司法问责与原来的错案责任追究明显不同。为构建科学、有效的新型司法问责制度,应当对司法问责的概念进行重新界定,并从司法问责的主体、司法问责的事由与对象、司法问责的标准与责任形式以及建立司法责任豁免制度等方面进行深入的研究。     

基于板蓝根中（R,S）-告伊春含量研究板蓝根加工方法

目的 研究不同干燥方法及软化切制方法对板蓝根中（R,S）-告伊春含量的影响,从而建立提升黑龙江产板蓝根质量的加工方法。方法 采用高效液相色谱法对板蓝根中（R,S）-告伊春含量进行测定,比较晒干与烘干板蓝根药材的（R,S）-告伊春含量差异,比较不同投料形式板蓝根颗粒和饮片中（R,S）-告伊春含量差异。结果 60 ℃烘干后的板蓝根药材中（R,S）-告伊春含量高于晒干的板蓝根药材,且能明显缩短板蓝根药材的产地干燥时间;经软化切制的板蓝根饮片中（R,S）-告伊春含量明显高于板蓝根粗颗粒。结论 在板蓝根产地加工过程中,可用烘干法代替晒干法;以板蓝根药材作为中成药投料,建议采用软化切制后的板蓝根饮片。     

化痰通瘀解毒方提取物对人胃癌细胞SGC-7901侵袭转移能力的影响

目的 观察化痰通瘀解毒方提取物对人胃癌细胞株SGC-7901侵袭转移能力的影响。方法 10、20、40 μg/mL化痰通瘀解毒方提取物分别干预人胃癌细胞SGC-7901 24 h后,采用Transwell小室法检测人胃癌细胞SGC-7901的侵袭、迁移能力,分别采用酶联免疫吸附试验和Western blot法检测SGC-7901细胞中基质金属蛋白酶9（matrix metalloproteinase 9, MMP-9）和E-钙黏蛋白（E-cadherin）的表达水平。结果 4组SGC-7901的侵袭细胞数和迁移细胞数以及E-cadherin和MMP-9表达水平比较,差异均具有统计学意义（P＜0.05）,呈现明显的剂量依赖性,以高剂量化痰解毒方提取物的效应最明显。结论 化痰解毒方可抑制人胃癌细胞SGC-7901侵袭和迁移能力,其作用机制可能与上调E-cadherin表达,下调MMP-9的表达有关。