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11.
为了建立适于鸭瘟病毒(DPV)弱毒增殖的细胞系,利用4个主要组织相容性复合体(MHC)单倍型鸭品系,体外分离鸭胚肝细胞,在干细胞因子、白细胞抑制因子和成纤维细胞生长因子诱导下,获得鸭胚肝间充质干细胞(DuLMSC)。接种DPV疫苗株C-KCE后,结果显示:F1~F10代均能稳定出现CPE;将F10病毒接种细胞24 h后,通过扫描电镜在胞质、胞核及细胞间隙均能够观察到典型的疱疹病毒粒子;F1至F10代均能扩增出DPV UL2基因片段,序列测定结果表明F1、F5、F10代的扩增产物与NCBI中公布的DPV株(EU082088)的核苷酸同源性为100%;组织培养半数感染量和一步生长曲线结果表明,源自B4系单倍型鸭的DuLMSC细胞系增殖DPV的病毒含量明显高于B1、B2和B3系。本研究为稳定培养鸭瘟病毒提供了优良稳定的实验材料。 相似文献
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Cinzia Greco 《Journal of Gender Studies》2016,25(3):303-317
This article analyses the areas of overlap between cosmetic surgery and reconstructive surgery, using the examples of both female and male breast surgery, and shows that these areas of overlap are sites of the construction of gendered bodies. The data are drawn from two pieces of research: the first based on 17 in-depth interviews with Italian cosmetic surgeons and the second based on 99 interviews with breast cancer patients and medical oncological professionals conducted in France and Italy. The primary data are supplemented by an analysis of the medical literature. ‘Too small’ female breasts (micromastia) and male gynecomastia (male fatty breast tissue) are pathologised by the surgeons and the medical literature, and a surgical intervention is presented as a way to heal this pathology. The pathologisation of healthy breasts goes along with the aestheticisation of oncological breast surgery. The interventions performed during a post-mastectomy breast reconstruction are guided by normative ideas of how a female body should be. The gender norms inscribed in this surgery practice can however be contested by patients: they can ask for a breast reconstruction while refusing all forms of ‘enhancement’. 相似文献
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Prior research finds that the emergence of a quality challenger is one of the most important factors predicting whether incumbents will be vulnerable. Reformers in California and Washington envisioned that the top-two primary reform would increase electoral competition by allowing for general election contests that feature two same-party candidates in safe districts. In this research note, I investigate the degree to which these expectations have been fulfilled by looking at the prevalence of quality challengers in U.S. House contests. I compare one-party and two-party general election contests, finding that incumbents are significantly more likely to face a quality challenger from the same party than from the opposite party, all else equal. In contrast, when both states used traditional primaries prior to reform, incumbents were no more likely to face a quality challenger in the primary election than in the general election. Findings reveal a key way in which the top-two primary may fulfill reformers’ expectations and complement our understanding of how electoral institutions condition challenger entry decisions. 相似文献
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The ‘pinkification’ of breast cancer culture in recent years conflates women’s empowerment with the celebration of hyperfemininity. Consistent with this trend, reconstructive surgery post-mastectomy is increasingly normalised: restoring the breasts is to restore ‘lost’ femininity. Contextualised within the pressures of this normalisation, our article explores how women who decide against breast reconstruction negotiate their non-normative ‘flat’ bodies. We examine women’s posts in a breast cancer forum about their refusals of breast reconstruction. Using thematic and feminist post-structuralist analyses, we suggest that although health and body acceptance discourses enable resistance to embodied femininity norms, pressures to conform permeate practices related to appearance. Clothes and prosthetic breasts enabled forum participants to pass as ‘healthy’, ‘whole’, and ‘recovered’. The study’s findings emphasise the limitations to agency and resistance that emanate from the ways constraining gender discourses infiltrate every aspect of a woman’s life. In line with a critical awareness approach to breast cancer education, we discuss the possibilities of resistance afforded by the safe spaces of online communities. 相似文献
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将360只1日龄天府肉鸭随机分为6组,分别喂以对照日粮(Cu 8 mg/kg)和高铜日粮(Ⅰ组:Cu100 mg/kg;Ⅱ组:Cu 200 mg/kg;Ⅲ组:Cu 400 mg/kg;Ⅳ组:Cu 600 mg/kg;Ⅴ组:Cu 800 mg/kg)6周,研究日粮铜添加水平对雏鸭肝及其相关生化指标的影响。结果显示,Ⅱ组、Ⅲ组、Ⅳ组、Ⅴ组雏鸭肝出现不同程度的病理损伤,肝细胞颗粒变性、空泡变性。Ⅳ组、Ⅴ组肝铜含量和血清铜含量均显著高于对照组(P<0.01)。Ⅲ组、Ⅳ组和Ⅴ组血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶活性显著升高(P<0.01)。结果表明,日粮铜含量达400~800 mg/kg即可引起肝的病理损伤。 相似文献
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人离体肝组织胺类产生与死亡时间和环境温度的关系 总被引:2,自引:1,他引:2
目的探讨在不同环境温度下,人离体肝组织中有机胺类产生量随死亡时间变化的规律,为某些碎尸案的死亡时间推断提供参考资料。方法应用高效液相色谱仪外标法,动态检测人离体肝组织中组胺、腐胺、尸胺和未腐败氨基酸含量。结果在8℃、15℃、23℃和32℃环境下,有机胺类产生量分别于人死后肝脏即刻离体69h、54h、40h和33h趋于最大值,并且在到达峰值之前,各温度组离体肝组织中有机胺类生成量均与死亡时间成正比关系。结论人离体肝组织中有机胺类产生量与死亡时间和环境温度在一定范围内均呈正相关。 相似文献
18.
目的:考察葛根和黄芩乙醇提取物体外抗乳腺癌细胞增殖活性。方法:葛根及黄芩200,400,700,950 m l/L乙醇提取物作用于人乳腺癌MCF-7(ER )和MDA-MB-231(ER-)细胞,MTT法检测对细胞增殖的影响。结果:葛根950 m l/L乙醇提取物在100~800μg/m l剂量均能显著抑制人乳腺癌细胞增殖,700 m l/L乙醇提取物也显示了一定的抗肿瘤细胞生长活性,200,400 m l/L乙醇提取物未见明显的抑制乳腺癌细胞增殖作用;黄芩950,700 m l/L乙醇提取物在50~400μg/m l剂量均能显著抑制人乳腺癌细胞增殖,400 m l/L乙醇提取物也显示了一定的抗肿瘤细胞生长活性,而200 m l/L乙醇提取物未见明显的抑制肿瘤细胞增殖作用。结论:葛根和黄芩乙醇提取物有一定的抑制乳腺癌细胞增殖作用。 相似文献
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A case of fatal Reye's syndrome presenting as the second sudden infant death in a family is described. The increasing interest in fatty change in the liver in cases of sudden infant death syndrome is noted and it is suggested that standardised interpretation of findings is essential if its significance is to be correctly evaluated. 相似文献