Individual assignment of body fluids in mixed stains by synonymous SNP analysis

Genotyping of transcribed single nucleotide polymorphisms (SNPs) in cDNA molecules derived from tissue-specific mRNAs has a potential for linking cell types to donors in mixed stains. To test this hypothesis, a single base extension assay targeting the synonymous SNP marker rs857870 in the myeloid cell nuclear differentiation antigen (MNDA) gene was designed. In blood/semen mixtures from donors with informative MNDA genotype (opposite homozygotes), only the MNDA allele of the blood contributor was observed in cDNA, even at blood/semen ratios of 1/20, corresponding to the limit of detection for mixtures by STR profiling. In blood/saliva mixtures, the MNDA allele of the saliva donor was sporadically seen in cDNA, probably due to trace amounts of blood in the donor's saliva, suggesting that the informativeness of the assay may be limited in presence of cell types with partially overlapping mRNA profiles.     

增强风险管理意识提高公安工作能力

在新形势下,风险问题已逐渐被社会各界所重视.公安工作任务、公安机关职责、公安工作方式的特殊性,使公安工作面临着与其他行政机关、司法机关不同的风险.因此,各级公安机关必须牢固树立正确的公安风险观,正确应对可能发生的风险,将风险管理作为警务战略运行和采取战术措施过程中的一个重要因素,从而不断提高公安工作能力.     

推动制度创新是提高构建和谐社会能力的关键

围绕构建和谐社会推动制度创新,必须着眼于“以人为本,”提高增强社会创造活力的能力;着眼于维护人民利益,提高正确处理人民内部矛盾的能力;着眼于社会资源的整合,提高推进社会管理体制创新的能力;着眼于软环境创新,提高维护社会稳定的能力;着眼于反映群众意愿,提高新形势下群众工作的能力。     

The Patients’ Rights Directive (2011/24/EU) – Providing (some) rights to EU residents seeking healthcare in other Member States

This article presents the main elements of Directive 2011/24/EU of the European Parliament and of the Council of 9 March 2011 on the application of patients’ rights in cross-border healthcare, commonly known as the Patient’s Rights Directive. It is the latest EU initiative with regard to European Health Care and the Single Market. The main elements of the Directive contain provisions related to the prior authorisation of health care in another Member State, the reimbursement of such health care and the removal of unjustified obstacles to achieving these aims.These provisions largely reflect the recent case law of the European Court of the Justice (ECJ). Amongst these are provisions involving the use of personal data. Such provisions will engage data protection issues and will have to be carried out according to the data protection directives. Alongside this primary aim of codifying ECJ case law the Patient’s Rights Directive also introduces novel initiatives aimed at fostering cross border cooperation between various elements of national healthcare systems.Part 1 of this contribution will describe the legal basis and the aims of the PRD, Part 2 will describe the principle obligations placed on the Member States with regard to reimbursement, Parts 3 and 4 will describe other informational and procedural requirements placed upon the Member States of Treatment and Affiliation. Finally Part 5 will outline some of the novel initiatives that have been included in the PRD.The increases in the frequency of cross border-treatment that this directive attempts to facilitate are likely to see a concurrent increase in cross-border patient information flows. Such data flows will be subject to the Union’s provisions on Data Protection. It remains uncertain whether the EU’s Data Protection regime will act as inhibitor to cross-border medical treatment or rather represent a gold standard that allows patients to engage in such activities with peace of mind. The Patient’s Rights Directive will form part of the EU’s future e-Health strategy which envisages a large increase in the fluidity of patient data. A discussion of this directive is therefore merited in this journal.     

The most ambitious plan in 26 years of telecoms market reform? No way!

The European Commission's claim that their proposed new telecoms regulation² constitutes “the most ambitious plan in 26 years of telecoms market reform” is preposterous. That honour must belong to the set of new European directives in 2002 which transformed the structure of telecoms regulation and facilitated competition throughout Europe. Instead the plans make great play of a number of actually pretty minor changes and, the Body for European Regulators for Electronic Communications (“BEREC”) has been particularly critical that the proposals will create unnecessary complexity and uncertainty and limit innovation and competition.     

Additional sequence characterization of NIST SRM 2391c: PCR-Based DNA Profiling Standard

The NIST Standard Reference Material (SRM) 2391c: PCR-Based DNA Profiling Standard was designed for use in the standardization of forensic and paternity quality assurance procedures for fragment-based typing short tandem repeat (STR) alleles generated by the polymerase chain reaction (PCR). Certified genotypes of the 6 components A–F were assigned for 24 autosomal and 17 Y-STR markers plus Amelogenin using concordance testing between commercial kits. Selected Sanger sequencing characterization was performed for the alleles of 11 STR markers when only one PCR primer set was available for fragment-based typing. The goal is to characterize the remaining 30 STR loci in components A–C by Sanger sequencing methods for the STR repeat regions and adjacent flanking regions. Additional characterization of the SRM is intended to support the emerging interest in next-generation sequencing technologies for forensic typing applications. Sanger methods have detected underlying polymorphisms (sequence, insertion-deletion, variation in complex motifs) typically not detected by fragment-based typing. The sequenced regions include the commercial or known PCR binding sites commonly implemented in fragment-based typing.     

小鼠心肌细胞核DNA变化与死亡时间的关系

目的监测小鼠死后细胞核DNA降解的情况,探讨死后细胞核DNA降解的一般规律。方法应用单细胞凝胶电泳(SCGE)技术结合荧光显微镜和专业的计算机图像分析技术,测定111只小鼠在死后72h内不同时间点小鼠心肌组织细胞头半径、尾长度、头DNA含量比例、尾DNA含量比例、尾矩、Olive矩、头面积、尾面积八项参数的变化值。结果在个体死亡72h内,测定的八项参数指标均显示细胞核DNA降解速率和程度与死亡时间具有高度的相关性,并获得了更能体现DNA降解趋势的二项式回归方程和多元回归方程,均具有高度的统计学意义。结论应用72h内心肌组织DNA变化与死亡时间之间呈线性关系的各组回归方程,可为法医学推断死后经过时间提供一种新的、客观的、精确的方法和参考依据。     

论新世纪加强党的执政能力建设的时代背景、科学依据及基本精神

加强党的执政能力建设,是进入新世纪以后国际国内的大形势对我们党提出的新要求。加强执政能力建设的科学依据是:巩固中国共产党执政地位的根本需要;实现党在新世纪历史使命和奋斗目标的客观需要;新世纪全面推进党的建设新的伟大工程的现实需要;提高党员领导干部能力素质的迫切需要。加强党的执政能力建设的基本精神包括:首先必须坚持正确的指导思想和总体目标;重点必须明确和掌握执政能力的基本任务和基本内容;根本措施就是全面推进党的建设新的伟大工程。     

中国社会保障法律体系的构想

分析了中国社会保障体系的立法背景,根据法律体系构建原理描述了中国社会保障的法律体系结构,指出我国社会保障体系应由政策优先向法律优先转移。从概念研究入手,立足于国际经验比较分析,根据中国宪法原则,提出了关于中国保障法律体系内容和结构的初步设想。