策划对提升报业传媒竞争力的作用

在报业传媒竞争激烈、同质化倾向严重的情况下,策划是突显传媒独特的定位与品牌特色、提高竞争力的重要手段。要培养从业人员的忧患、竞争意识和信息敏感度,以受众为本,善于思考和创新。注意真实性、不炒作、避免玩策划游戏和不健康策划、过分夸大策划作用等行为。     

跨文化交际能力与大学外语教学

目前我国大学外语教学中普遍存在着过度注重语言技能而忽视语言文化属性的现象,结果导致语言习得效率低下,学生跨文化交际能力弱化。应从师资培养、教学内容、课堂设计、学习方法、资源利用等方面入手,切实提高外语教学质量。     

单细胞凝胶电泳检测大鼠死后肝细胞核DNA降解

目的应用单细胞凝胶电泳技术(SCGE)检测大鼠死后肝细胞核DNA降解规律,分析与死亡时间的关系,为早期死亡时间的推断提供新的方法。方法在大鼠死后30h内,每隔3h取肝组织样本进行单细胞凝胶电泳,用共聚焦显微镜摄取彗星图像,应用彗星图像分析软件(IM I1.0)进行图像分析,并作统计学分析。结果死后大鼠的肝细胞在电泳图像上出现明显的彗星形拖尾,其尾长(TL)、尾矩(TM)在一定的时间范围内(0~18h)随死亡时间的延长而逐渐增大,二者均与死亡时间(PM I)呈现一定的相关回归关系。结论单细胞凝胶电泳技术可应用于早期死亡时间的推断。     

加强地方党委执政能力建设的几点思考

地方党委必须从本地区实际出发,确立科学的执政理念,强化执政意识,抓好执政兴国的第一要务,提高执政绩效,改革和完善党的领导体制和工作机制,加强干部队伍建设,强化执政主体素质,密切党同人民群众的血肉联系,塑造良好执政形象,最广泛最充分地动员执政资源。     

Forensic typing of autosomal SNPs with a 29 SNP-multiplex—Results of a collaborative EDNAP exercise

We report the results of an inter-laboratory exercise on typing of autosomal single nucleotide polymorphisms (SNP) for forensic genetic investigations in crime cases. The European DNA Profiling Group (EDNAP), a working group under the International Society for Forensic Genetics (ISFG), organised the exercise. A total of 11 European and one US forensic genetic laboratories tested a subset of a 52 SNP-multiplex PCR kit developed by the SNPforID consortium. The 52 SNP-multiplex kit amplifies 52 DNA fragments with 52 autosomal SNP loci in one multiplex PCR. The 52 SNPs are detected in two separate single base extension (SBE) multiplex reactions with 29 and 23 SNPs, respectively, using SNaPshot kit, capillary electrophoresis and multicolour fluorescence detection. For practical reasons, only the 29 SBE multiplex reaction was carried out by the participating laboratories. A total of 11 bloodstains on FTA cards including a sample of poor quality and a negative control were sent to the laboratories together with the essential reagents for the initial multiplex PCR and the multiplex SBE reaction. The total SNP locus dropout rate was 2.8% and more than 50% of the dropouts were observed with the poor quality sample. The overall rate of discrepant SNP allele assignments was 2.0%. Two laboratories reported 60% of all the discrepancies. Two laboratories reported all 29 SNP alleles in all 10 positive samples correctly. The results of the collaborative exercise were surprisingly good and demonstrate that SNP typing with SBE, capillary electrophoresis and multicolour detection methods can be developed for forensic genetics.     

和谐社会视角下的中国政府社会政策能力

社会政策是和谐社会发展的制度保障。社会政策作为社会运行机制中的平衡机制,提高社会政策能力对于构建社会主义和谐社会起着举足轻重的作用。和谐社会语境中应然状态下政府社会政策能力包括维护社会公正,协调利益矛盾的能力;敏感的社会政策意识的能力;高屋建瓴的规划社会政策的能力;有效实施社会政策的能力。中国政府社会政策能力的实然状态与应然状态相比,差距很大,需要提升政府社会政策能力,使其从实然状态向应然状态的转化。     

用dHPLC技术检测线粒体DNA编码区单核苷酸多态性

目的研究线粒体DNA(m tDNA)编码区单核苷酸多态性,建立检测m tDNA编码区单核苷酸多态性(SNP)的变性高效液相色谱(dHPLC)方法。方法设计针对线粒体DNA编码区nt10287-10679及nt8507-8805引物,应用dHPLC技术检测其序列多态性。结果100例中国汉族无关个体中,m tDNA nt10287-10679检出13个SNP位点,13种单倍型,基因多样性(H)为70.79%,偶合概率(P)为29.92%;m tDNA nt8507-8805检出10个SNP位点,12种单倍型,H为70.42%,P为30.28%;两段序列联合起来共检出23个SNP位点,23种单倍型,H为84.14%,P为16.70%。结论所建立的dHPLC方法可用于快速、准确地检测m tDNA编码区序列多态性;m tDNA编码区多态性位点作为m tDNA控制区多态性位点的补充,联合应用可以提高m tDNA的个体识别能力。     

综论印度文化的特点

同中国文化一样,印度文化也是世界上最古老的文明体系之一。它起源于公元前2500年左右的印度河流域,至今已有4500多年的历史。印度文化历经风风雨雨,起伏跌宕,从未中断,一直延续至今。印度文化源远流长、历史悠久、博大精深,内容和形式丰富而多彩。由于印度地处特殊的自然地理环境,并且具有自己独特的社会人文发展背景,所以印度文化有着许多其他民族文化所没有的特点。印度文化有它的八大特点:一是一个多元文化的统一体。印度自古就是一个多宗教、多种族、多种语言并存的国度,有一种"印度精神"把他们凝聚在一起。二是具有强大的融合和同化能力。三是信仰人与自然和谐统一的世界观。四是重精神、轻物质的人生价值观。五是在思维方式上,追求永恒精神、崇尚内向直觉思维。六是由于宗教文化的影响,古代印度人不注重历史,历史观念淡薄。七是印度自古以来就形成了一种等级分离制度,后来发展为种姓制度,这种制度对印度社会和文化都产生了深远影响。八是印度古代就发明了瑜伽术,后被各种宗教所吸纳,作为它们实现精神解脱的手段。现在,瑜伽术已演化为成一种修心养性、强身健体的体育项目风靡于世界。     

Population data for 94 identity SNPs in four U.S. population groups

The U.S. National Institute of Standards and Technology (NIST) sequenced 1036 human DNA samples from four United States population groups (African American, Asian, Hispanic, and Caucasian) using the ForenSeq DNA Signature Prep Kit with Primer Mix B (DPMB) on a MiSeq FGx instrument. In addition to STR markers, DPMB includes amplification primers for single nucleotide polymorphisms (SNPs) used for individual identification (iiSNPs, n = 94), ancestry inference (aiSNPs, n = 56), and phenotype prediction (piSNPs, n = 22). Resulting sequencing coverage information was interpreted for the 94 iiSNP markers. Here we present performance characteristics of the ForenSeq DNA Signature Prep Kit in the population studied.     

Whitelisting system state in windows forensic memory visualizations

Examiners in the field of digital forensics regularly encounter enormous amounts of data and must identify the few artifacts of evidentiary value. One challenge these examiners face is manual reconstruction of complex datasets with both hierarchical and associative relationships. The complexity of this data requires significant knowledge, training, and experience to correctly and efficiently examine. Current methods provide text-based representations or low-level visualizations, but levee the task of maintaining global context of system state on the examiner. This research presents a visualization tool that improves analysis methods through simultaneous representation of the hierarchical and associative relationships and local detailed data within a single page application. A novel whitelisting feature further improves analysis by eliminating items of less interest from view. Results from a pilot study demonstrate that the visualization tool can assist examiners to more accurately and quickly identify artifacts of interest.