mRNA荧光复合扩增系统鉴别正常和异常精液初探

目的构建mRNA荧光复合扩增体系,实现对不同种类精液(尤其是无精症精液)的区分鉴别。方法收集正常、少精症及无精症的精液样本,制备精斑样本后提取细胞总RNA,利用逆转录PCR技术扩增2个精子特异mRNA标记(PRM1、PRM2)、2个精浆特异mRNA标记(TGM4、SEMG1)和2个管家基因mRNA标记(TEF、UCE)。结果正常精液样本可检测到全部精液mRNA标记表达;少精症精液样本虽然可检测到全部mRNA标记表达,但精子特异mRNA标记表达量较低;无精症精液样本不能检测到精子mRNA标记,只能检测到精浆特异mRNA标记。结论利用mRNA荧光复合扩增系统可以实现对正常和无精症精液的区分,而正常精液和少精症精液相比差异无统计学意义。     

English literacy： Consuming the Kapampangan oral antecedent

This paper discusses the effects of American colonial rule on the cultural life of the Kapampangans and inhabitants of the province of Pampanga. The colonial masters, who seemed to deliberately marginalize the local language of the community and succeeded in eventually arresting its natural development. The American policy to ＂educate＂ the Filipinos resulted in the waning of the Kapampangan language and literature. A brief overview of Kapampangan literary history will be given to emphasize the folk tradition, the oral nature of Kapampangan verbal art as well as the worldview and ethos of the people embedded in their literature. To be mentioned are two Kapampangan writers, Crisostomo Soto and Jose Gallardo, who represented the periods during and after the American regime respectively. Each responded to his time and scene; both struggled against the most powerful colonial machine which was the English language. They were aware that this medium and its message were one, and that their own language would, sooner or later, be a lost medium and a lost message. The shift from orality to literacy, as a consequence of western education, will also be discussed. In particular, the reaction of Kapampangan verbal artists to the reductive pressure exerted by literacy upon their literature will be focused.     

Historical tradition and community mobilisation: narratives of Red Clydeside in memories of the anti-poll tax movement in Scotland, 1988–1990

Contemporary scholarship has shifted focus from a ‘labour history’ focused on industrial movements to a more comprehensive ‘working-class history’, encompassing the broader social parameters of protest with community and industrial struggles unified in material interest and consciousness. This article locates the poll tax non-payment campaign of 1988–1990 on Clydeside, a major expression of working-class mobilisation which contributed to the demise of Margaret Thatcher’s premiership, within this international historiography. The analysis is based on oral history interviews with twelve activists who represented all the major political trends from the non-payment campaign. The anti-poll tax movement was embedded in traditions of community mobilisation shaped by a moral economy of housing and amenities, which had roots in the First World War era ‘Red Clydeside’ struggles, and developed through the post-Second World War predominance of public sector housing. The analysis demonstrates how activists constructed narratives of their own resistance in the anti-poll tax movement within a powerful cultural circuit, where the collective memory of past mobilisations and the consciousness associated with the moral economy of housing and amenities informed contemporary perspectives and political activity. The campaign was not politically monocultural. Differences between political groups involved in the non-payment campaign are analysed showing that the need of composure (of memories) led to contrasting interpretations of Red Clydeside. These were influenced by geographical distinctions between traditional working-class areas with strong tenants’ organisations and the peripheral estates where such organisation was weaker. The impact of deindustrialisation and the welfare policies of the Thatcher government created a popular resentment in these areas. This strengthened moral economy opposition to the poll tax, whilst the traditions of community mobilisation provided effective means of harnessing this through non-payment and direct action against sheriff officers.     

India＇s Look East Policy and north-eastern states： An overview

To become an economic developed country, every country needs to look for its trade and commerce because it is one of the most prominent factors. Hence, the present study is an endeavor to highlight ＂India＇s Look East Policy and its northeastern states＂. The methodology adopted here is mainly based on secondary data and information, the sources of which have been compiled from different national and international journals and related books, etc.. India＇s involvement in the enterprises of ASEAN （Association of South East Asian Nations） for economic corporation was initiated in the year 1991 with the function of ＂Look East Policy＂. ASEAN countries have emerged as significant entities among India＇s most dynamic trade partners. Undoubtedly, north-east India has the potential to become gateway to the east, which can help trade and commerce with ASEAN countries to flourish.     

道德话语系统与压力型司法的路径选择

面对道德困境案件的日趋增多,司法之道德话语系统的社会意义得以凸现,法官往往需要将目光往返于法律话语系统与道德话语系统,充分反映民众的共同道德观,并自觉拒斥虚妄的民意诉求对司法的消极影响。当然,也使难办案件意义上的司法裁判被简缩为冲击—回应的被动过程,从而形成了一种基于外在压力影响但又需要慎重对待的压力型司法。如果现代司法放弃建立纯而又纯的法条主义之梦,而将道德话语系统融入司法的价值判断,那么压力型司法将在以道德论证弥补法律之确定性的裂缝以及以判决书说理制度增加司法判决的可接受性之方法选择中走出困境。     

Rapid enantiomeric analysis of zopiclone in serum by supercritical fluid chromatography–tandem mass spectrometry

Zopiclone (ZOP) is a hypnotic drug prescribed to treat insomnia. Due to the chiral nature of ZOP, the psychologically active S-form and inactive R-form need to be determined enantiomerically in a forensic drug analysis. In the present study, a supercritical fluid chromatography (SFC) method was designed with a faster analysis ability than that of previously reported techniques. The SFC–tandem mass spectrometry (SFC–MS/MS) method was optimized using a column with a chiral polysaccharide stationary phase (Trefoil CEL2). ZOP was extracted from pooled human serum using solid-phase extraction (Oasis HLB) and analyzed. The developed SFC–MS/MS method achieved the baseline separation of S-ZOP and R-ZOP within 2 min. The fit-for-purpose method validation indicated that the optimized solid-phase extraction achieved near complete recovery and approximately 70% of the matrix effect. Both the retention time and peak area showed sufficient precision. The lower and upper limits of quantification (LOQ) were 5.7 × 10⁻² ng/mL and 25 ng/mL for R-ZOP, and 5.2 × 10⁻² ng/mL and 25 ng/mL for S-ZOP. The calibration line was linear in the range from lower LOQ to upper LOQ. The stability test indicated that ZOP in serum stored in a refrigerator (4°C) degraded and about 55% remained in 31 days. The quick analysis of the SFC–MS/MS method makes it a valid option for the enantiomeric analysis of ZOP.     

Examining the transfer of male DNA onto female underwear in simulated non sexual social interaction versus digital penetration to assist in the evaluation of results in casework scenarios

Experiments have been carried out by the UK and Ireland Association of Forensic Science Providers Body Fluid Forum (AFSP BFF) to determine the levels of male DNA, detected during Y-STR analysis, that may be expected on female underwear from non-sexual social interaction and digital penetration, versus non-sexual social interaction only. The data obtained strongly supports the existing assumptions made: whilst low levels of DNA may be inadvertently transferred to the inside surface of a female’s underwear during social interaction with a male, there is a low expectation of detecting a matching Y-STR profile to that male, which is suitable for statistical evaluation, unless he is a co-habitant of that female.     

非法言词证据之认定标准

我国2012年刑事诉讼法正式确立了非法证据排除规则,并设定了以手段为重要内容的“非法方法”判断标准。根据刑事诉讼法和相关的司法解释,非法证据排除设立之目的在于规范司法行为和办案程序,惩罚犯罪和保障人权。以“非法方法”作为非法言词证据的判断标准并不能够满足该制度之目的与要求,应当以程序合法为前提、自白规则为要素、人权保障为主线,综合进行。     

The development of a method for FISH identification of forensically relevant body fluids

Messenger RNA profiling is a useful confirmatory test for body fluid identification, but there are limitations to this method including sensitivity and the difficulty in linking body fluids with the corresponding DNA profile in mixed samples. We have developed a method for RNA suspension fluorescence in situ hybridisation (RNA S-FISH) for forensic-type samples using locked nucleic acid (LNA) probes. Vaginal and buccal epithelial cells have been the primary focus, with some preliminary work performed on leucocytes and seminal round cells. We have designed probes for the KRT10 messenger RNA and the micro RNA 891a. Using these probes, we have optimised a RNA S-FISH methodology and have successfully visualised these cell types using fluorescent microscopy.