Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

警力资源分类管理机制建设的探索与思考

在警力资源配置中引入分类管理机制,对进一步优化警力资源配置及实现其无增长改善,具有十分重要的意义。为此,必须探索和完善岗位分类制度、警力资源配置和流动机制、警力绩效考评机制、激励保障机制和警力辅助机制。     

积极发挥审计监督不可替代的重要作用努力构建有公安特色的惩治和预防腐败体系

公安审计队伍作为一支独立的监督力量,在公安业务、队伍和党风廉政建设中发挥了重要作用,为公安事业作出了应有贡献.在构建有公安特色的惩防体系过程中,应继续按照公安机关大监督格局的路子,进一步发挥审计职能作用,坚持发挥审计工作的"三个效益",努力形成预防有效、监督及时、惩治有力的惩防体系.     

解析派出所培训学习与实战镶嵌的教学模式

公安大教育观是建立在现代社会的科学、经济、政治、文化高度发展基础上的一种现代教育观。公安大教育把追求自我学习、自我提高作为民警向知识进军的一种动机。公安大教育应开展协同式教学模式,注重兼职教官的连结功能;斟酌业务教材,创建新颖“学员包”,抓好微型教学。     

赌博关联犯罪的认定与处理

近年来,我国赌博犯罪势头高涨,危害性日益增大。赌博犯罪往往诱发其他各类犯罪,产生赌博关联犯罪。由于赌博犯罪与相关关联犯罪错综交织、互相推动,因此对赌博关联犯罪的认定就显得更加困难和复杂。     

新形势下完善《国籍法》的思考

《中华人民共和国国籍法》公布实施至今已二十六年。随着我国对外开放的进一步发展,国际交往日益增多,新的国籍问题也时有出现。由于《国籍法》独立性较强,在司法实践中,一些特殊案例又无法借用其他相应的法律,因此,迫切需要对《国籍法》加以完善和修改,同时还要规范执法。     

股东知情权诉讼中存在的实务问题及思考

获取公司信息的股东知情权既是股东的一项重要权利,也是股东行使其他股东权利的必要手段和媒介。但在董事会和管理层的权利日益膨胀的今天,股东对公司信息的知情权却受到了日益严重的忽视和排挤,并由此引发了诸多纠纷。因此,有必要在对审判实践中股东知情权案件审理中出现的问题进行归整的基础上,就股东知情权的诉讼主体、知情权范围、限制及行使程序等问题作一探讨,从而达到维护股东权益的目的。     

关于交警信息化建设和应用的思考

交通管理信息化建设和应用,是整个公安系统信息化工作的重要组成部分,具有涉及面广、信息量大、信息变化快、重要性强等特点。制约交警信息化建设和应用工作的因素仍然存在。交警部门要抓住当前"三基"工程建设的有利时机,加强信息化建设,推动基层基础工作,为交通管理和民警执法提供科技服务,从而实现在信息化主导下交通管理模式的转变。     

关于塑造"亮剑"精神提升基层公安队伍管理效能的思考

当前,基层公安队伍最需要塑造的"亮剑"精神是"胸怀人民、忠诚敬业的信念;精诚团结、和谐凝聚的作风;主动进取、迎难而上的胆略;坚韧执着、锲而不舍的毅力;专注于心、踏实有为的品质".塑造"亮剑"精神是一项长期性、系统性、基础性工程,必须立足新的时代背景和基层公安具体实践,以建机制、出实招为突破口,围绕"引导、训练、规范、凝聚"八个字,综合运用多维塑造法,持之以恒、不断完善,才能形成和强化这一精神优势,为基层公安工作注入持久的动力和活力.     

构建与新型社区管理相匹配的警务机制

针对基层派出所防范管理主业还未完全凸显,基础工作信息体系还未完全构筑,群防群治网络还未合理严谨的问题,应通过着力推进社区治安巡访机制,强化情报信息和人口管理意识,以构建群防群治网络,严密基层的阵地控制,整治治安问题突出地区,继而调整管理防范的薄弱部位,实施新型社区警务战略。