柔嫩艾美球虫pEtK2抗原基因的克隆表达及其表达产物的抗原性分析

应用RT-PCR技术从纯化的柔嫩艾美球虫孢子化卵囊子孢子中克隆了pEtK2抗原基因,并进行了原核表达和抗原性分析,同时用MTT法检测了重组蛋白对球虫耐过鸡淋巴细胞的刺激效果。结果显示,克隆的pEtK2基因全长1464 bp,具有一个完整的开放阅读框,编码487个氨基酸,具有多个T细胞表位。重组蛋白诱导5 h后表达量最多,分子质量约55 ku,占菌体总蛋白的32%。纯化的重组蛋白能够刺激球虫耐过鸡T淋巴细胞增殖,可作为球虫基因工程疫苗或DNA疫苗的候选基因。     

法眼看辽宁西丰“拘传记者案”

辽宁西丰"拘传记者案"是地方公共权力侵犯公民宪法权利,侵害传媒新闻自由权,侵犯记者人身自由权的典型案例。该案例对我们的启示是,应着重在制度层面上调整地方党政机关职权划分和完善党对地方事务,尤其是司法事务的领导方式;在法律层面上进一步完善《刑法》、《刑事诉讼法》等相关法律,细化公安机关和检察机关司法行为的法定要件,特别是要尽快完善关于记者权利保护的立法等,以更好地保障新闻媒体和社会大众对我国各级国家机关及其工作人员进行舆论监督。     

PDGF-β,PDGFR-β,TGF-β1,bFGF在创伤愈合过程中的表达变化与损伤时间关系的研究

目的研究细胞因子在创伤及修复过程中的表达变化与损伤时间 ( 伤口年龄 ) 的关系 . 方法用免疫组织化学技术和图像分析技术 , 对实验大鼠不同损伤时间的生前伤 ( 0.5～ 168h am) 及死后伤 ( 0.5～ 6h pm) 皮肤创缘组织中细胞因子和受体的表达变化进行了研究 . 结果在生前伤 , PDGF- β , PDGFR- β , TGF- β 1, bFGF等细胞因子在上皮细胞的表达于损伤后 0.5h am 即开始增强 , 其中以 24～ 96h am反应最强 . TGF- β 1, bFGF 以及 PDGF- β除了在上皮细胞的表达外 , 还大量出现在肉芽组织的巨噬细胞和成纤维细胞内 , 亦以 24～ 96h am 最明显 , 而死后损伤组上述因子仅在 0.5～ 3h以内有弱表达 , 3h后则均无任何表达 . 结论 PDGF- β , PDGFR- β , TGF- β 1, bFGF在创伤愈合过程中的变化及特点与损伤时间的关系可作为推断损伤时间的免疫病理学标志 .     

复发性流产肾虚证小鼠子宫蜕膜组织miRNA表达谱检测及生物信息学分析

目的 探讨微小RNA（microRNA,miRNA）在复发性流产（repeated spontaneous abortion,RSA）肾虚证小鼠子宫蜕膜组织中的表达特征。方法 运用基因芯片技术对所采集的正常对照组和RSA小鼠蜕膜组织进行miRNA检测,运用Genespring软件对miRNA进行筛选及功能分析。应用生物信息学方法分析差异表达的miRNA及其靶向基因在RSA发生、发展中的作用。结果 芯片检测结果显示正常对照组和RSA肾虚证组筛选出差异表达的miRNA共有63条（22条上调、41条下调）,实时荧光定量PCR验证的miRNA的表达情况与miRNA芯片结果一致。京都基因与基因组百科全书通路富集分析及基因本体分析显示,其差异表达的miRNA涉及到PI3K-Akt信号通路、细胞吞噬通路、癌症通路、黏着斑通路、肌动蛋白细胞骨架调控通路、轴突导向信号、MAPK信号通路、PAP-1信号通路等。结论 RSA肾虚证小鼠子宫蜕膜组织中存在差异表达的micro RNA,可能涉及RSA肾虚证的发生、发展。     

非洲猪瘟CD2v胞外区基因片段的真核表达及其多克隆抗体的制备

本研究利用真核表达系统表达非洲猪瘟病毒的CD2v胞外区蛋白,研究其免疫特性,制备多克隆抗体并研究其性质,为研发特异性单抗奠定数据基础.以非洲猪瘟病毒Wuhan2019-1株(GenBank:MN393476.1)为模板,合成CD2v胞外区基因序列,并将其克隆至真核表达载体pCAGGS载体中,获得pC-AGGS-CD2v...     

The Impact of Political Change on the State of Academia Including Academic Freedom in the Arab World: Libya as a Case Study

ABSTRACT

Furthering Western style academic freedom has been challenging, as Arab countries, especially Libya, have known only autocratic regimes throughout their modern existence. Amidst its current political and social upheaval, Libyan society is drifting towards the unknown. The problem addressed in this study is the impact of political change on the state of academia but, more specifically, academic freedom. Since the intervention in Libya by NATO states, many academics have lost their jobs. Some have become refugees outside of Libya as a direct result of the appropriation of most of the governmental posts in the country by religious and political radicals. The research questions reflect ways in which the new inserted de facto leaders of post-transitional Libya have impacted life on Libyan campuses and academic freedom.     

Beliefs in a Just World as a Buffer Against Anger

Anger is the emotion typically accompanying unfairness appraisals. Belief in a just world (BJW) was expected to buffer against angry feelings because subjects high in BJW play down unfairness, justify unfairness as being self-inflicted, and avoid self-focused rumination. Three studies were run to test this hypothesis. A questionnaire study revealed that subjects who strongly endorse the belief in a personally just world are less likely to experience anger and, if they do experience such feelings, are less likely to suppress them. In two experiments, an angry, a happy, or a sad mood was induced. In the anger-evoking condition, subjects high in BJW were less angry and suffered no decrease in self-esteem compared to subjects low in BJW, who reported increased feelings of anger and decreased self-esteem. It is concluded that individuals high in BJW are better able to cope with anger-evoking situations, and that BJW can be seen as a personal resource protecting not only mental but also physical health.     

言论与行为二元论：表达自由立场上的刑法行为理论考量

历来对思想的控制无不是以对言论的控制为表征和途径的,自古罗马以来,“任何人不因思想受处罚”的法律格言,从未阻绝此后漫长历史时期统治者对言论自由的钳制。刑法学家将发表言论一概解释为行为,非但无法保障思想自由,可能与其初衷正相反;借鉴美国判例中“言论——行为”两分法,将具有表达意义的行为在一定条件下解释为言论,不仅是对宪法中言论自由原则的呼应,而且对思想自由保护的现实意义也是深远的。     

H5亚型禽流感病毒核蛋白基因重组质粒的构建及其在Hela细胞的瞬时表达

采用PCR技术从重组质粒pMel-NP扩增得到了禽流感病毒(AIV)A/Goose/Guang-dong/1/96(H5N1)株的核蛋白(NP)基因,将其亚克隆至真核表达载体pVAX1上。将提取的pVAX-NP阳性克隆转染Hela细胞,48 h后经间接免疫荧光试验和Western-blotting检测,NP基因在Hela细胞中已成功瞬时表达。表达产物的分子质量约为57 ku,它与H5N1亚型AIV多克隆抗血清有较好的免疫反应。     

什么是言论——《宪法》第35条的一种论理解释

综合我国基本权利谱系、基本权利条款间的关系及立法意图,既不宜对言论作字面解释,将其含义限定为口头形式,同时也不宜作过分的扩充解释。我国《宪法》第35条意义上的言论,不仅包括口头形式,还应包括书面形式,但已成为基本权利或可以被其他基本权利条款所包摄的表达形式不在言论范畴。