A multifaceted,multijurisdictional, multiagency,and multidisciplinary approach to investigating unidentified and missing persons cases in Australia

Currently, there are approximately 750 unidentified human remains and 2500 long-term missing persons in Australia. The Australian Federal Police National DNA Program for Unidentified and Missing Persons (Program) is using a multifaceted, multijurisdictional, multiagency, and multidisciplinary approach in a dedicated effort to identify these unknown deceased persons, scientifically link them to known missing persons, and provide answers to their families. The nationally coordinated Program provides its police, forensic, and coronial stakeholders with a suite of contemporary forensic technologies, databases, and experts to forensically examine the skeletonised remains and recover post-mortem data for comparison to the available ante-mortem data for each missing person. Through a number of physical and virtual public outreach activities, families with missing relatives have been encouraged to provide vital ante-mortem forensic information, records, and samples to aid the identification process. To date, this unique Program has assisted to resolve a number of unidentified and missing persons cases from both historical and contemporary contexts, using a combination of genetic and non-genetic techniques, and local and national databases. The centralisation of Program capabilities, expertise, and resources to conduct this type of unique and challenging casework is proving to be the most effective and efficient way to generate investigative leads, identify human remains, and resolve long-term missing persons cases in Australia.     

党的组织法基本问题研究

党的组织法是党内法规中最基础、最重要的规范,其对于从严治党及增强党的执政能力、推动国家治理现代化和法治中国建设具有重要意义。党的组织法是调整与规范党组织及其运行的规范之集合,具有独特的系统建构功能和规范功能,对党的建设和党的执政能力的提升具有重要价值。当下中国正处在改革转型和走向世界的关键时期,机遇与挑战并存,党的组织法建设要服务于中国治理模式的构建,为党的执政领导提供有效的组织支撑,同时要提高党的自我治理能力并规范其组织权力的运行。此外,现行分散的组织法规范需要进行梳理修订,以加强党的组织法体系化建设,更好发挥党的组织法的基础作用。     

Addition of an adipose marker to the organ tissue identification mRNA assay

At the Netherlands Forensic Institute, mRNA profiling for the purpose of organ tissue identification is frequently requested in casework. The previous version of the assay (denoted Organtyper) lacked markers for identifying adipose tissue, while this was occasionally questioned in casework. Here, three potential adipose markers are examined before incorporating one into the final Organtyper assay. The added value of the adipose marker becomes clear from looking at casework results.     

Oral and skin microbiome as potential tools in forensic field

The interest in the analysis of the human microbiome for personal identification purposes is based on the microbial diversity amongst individuals. The oral cavity hosts one of the most diverse and abundant microbial communities in the human body; the skin instead is a complex living ecosystem with unique microbial niches at different sites. Both skin and oral microbiomes are highly individual and relatively stable over time. As saliva and skin debris are often found at crime scenes, the analysis of their microbiome may represent a potential tool for personal identification. However, there are some gaps in knowledge on how factors such as age, sex, geographic origin, diet and pathologies can affect the composition of the microbiome. The aim of this study is to improve the existing knowledge by examining oral and skin microbiomes from the same individuals and evaluating the variability between anatomical sites and donors. For this study, 50 individuals living in Italy donated oral swab samples and provided information regarding their diet, lifestyle, health status, antibiotic use, and other demographic data. Skin swabs from 11 of the 50 individuals were also analysed and compared to the oral swabs from the same donors. All analyses were done through metabarcoding of the 16S rRNA region of DNA extracted from the samples. This research outlines the potential use of oral and skin microbiome signatures as added evidence in personal identification, providing useful investigative clues for future forensic caseworks.     

Molecular identification of forensically important fly species in Spain using COI barcodes

Species identification with DNA barcodes has been proven to be effective on different organisms and, particularly, has become a routinely used and quite accurate tool in forensic entomology to study necrophagous Diptera species. In this study, we analysed 215 specimens belonging to 42 species of 17 genera, from 9 different Diptera families. Flies were collected in 39 Spanish localities of the Iberian Peninsula sampled across three years in the four seasons. Intraspecific variation ranged from 0 to 2.46% whereas interspecific variation fluctuated from 3.07 to 14.59%, measuring 651 pb of the cytochrome oxidase subunit one (COI) gene. Neighbour-Joining analysis was carried out to investigate the molecular identification capabilities of the barcoding region, recovering almost all species as distinct monophyletic groups. The species groupings were generally consistent with morphological and molecular identifications. This work, which is the first with this intensive and extensive sampling in this area, shows that the COI barcode is an appropriate marker for unambiguous identification of forensically important Diptera in Spain.     

Detection of microRNAs in DNA Extractions for Forensic Biological Source Identification

Molecular‐based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT‐qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA‐specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva.     

Validation of the 4‐aminophenol color test for the differentiation of marijuana‐type and hemp‐type cannabis

The analysis of cannabis plant material submitted to seized‐drug laboratories was significantly affected by the signing of the Agricultural Improvement Act of 2018, which defined hemp and removed it from the definition of marijuana in the Controlled Substances Act. As a result, field law enforcement personnel and forensic laboratories now are in need of implementing new protocols that can distinguish between marijuana‐type and hemp‐type cannabis. Colorimetric tests provide a cost‐effective and efficient manner to presumptively identify materials prior to submission to a laboratory for analysis. This work presents the validation of the 4‐aminophenol (4‐AP) color test and demonstrates its utility for discriminating between marijuana‐type and hemp‐type cannabis (i.e., typification). Validation studies included the testing of numerous cannabinoid reference materials, household herbs, previously characterized cannabis plant samples, and real‐case samples. The 4‐AP test reliably produces a pink result when the level of Δ⁹‐tetrahydrocannabinol (THC) is approximately three times lower than the level of cannabidiol (CBD). A blue result is generated when the level of THC is approximately three times higher than that of CBD. Inconclusive results are observed when the levels of THC and CBD are within a factor of three from each other, demonstrating the limitations of the test under those scenarios.     

Character Weakness,Partisan Bias,and Presidential Evaluation: Modifications and Extensions

In a recent article Goren (American Journal of Political Science, 46, 627–641, 2002) draws upon theories of negativity bias, partisan bias, and motivated reasoning to posit that the more strongly people identify with the opposition party of a presidential candidate, the more heavily they will rely on character weakness impressions to construct global candidate evaluations. This paper modifies the theoretical framework by positing that (1) partisans will judge opposition nominees most critically on the traits owned by the former’s party and (2) partisan bias promotes negativity bias in the evaluation of incumbent presidents seeking reelection and incumbent vice presidents seeking the presidency. Analysis of data from the 2000 and 2004 NES surveys, along with a reconsideration of the results from the 1984 to 1996 period covered in the original piece, yields strong empirical support for these expectations.

Explaining Turnout Decline in Britain, 1964–2005: Party Identification and the Political Context

Turnout decline in Britain is greater than it first appears since changes in the social composition of the electorate have had a positive impact on turnout. This paper finds that whereas a weakening in the strength of party identification is associated with the long-term decline, the political context influences short-term variation. Partisan dealignment is also changing the dynamics of the determinants of turnout. Since non-identifiers are more strongly influenced by the political context than strong identifiers, and there are now more non-identifiers than previously, the political context is becoming a more important factor in determining whether people vote or not.

Identification and analysis of human remains recovered from wells from the 1991 War in Croatia

From 1996 to the present, the remains of 61 individuals killed during the 1991 War in Croatia were recovered from both dried out and functioning wells. Positive identification was established in 60.7% or 37/61 cases. Remains recovered from the same geographical region but from non-well settings were identified in 77.4% or 1256/1623 cases. The purpose of this paper is to report on the taphonomic, demographic and trauma characteristics of remains recovered from wells and identify factors responsible for the discrepancy in the identification ratios. The age and sex distributions in the well and non-well series were similar, as were the frequencies of recovered personal documents, jewelry and other artifacts. The taphonomic features of the remains were, however, significantly different. Preservation of remains was considerably better in non-well settings (1400/1623 or 86.3% compared to 40/61 or 65.5% in wells). Congruently, commingling of remains was more frequent in wells (26/61 or 42.6% compared to 77/1623 or 4.7% in non-well settings). In bodies recovered from non-well settings the preservation, state and commingling of the remains were strongly correlated with positive identification. None of these features were correlated with the identification of bodies from wells. Instead, identification of remains from wells was significantly affected by the presence or absence of water in the well. As both series have similar frequencies, and identical rankings of identification factors, the reason for the discrepancy in the identification ratios lies in the fact that by themselves, these factors were rarely sufficient for positive identification. In both series the majority of identifications (51.4% in the well, and 58.1% in the non-well series) were established through a combination of biological and non-biological features. The significantly lower identification ratio in the well series resulted from the difficult recovery conditions in wells with significant amounts of water, and the negative effect that water had on the preservation of clothing, personal artifacts and some dental features. Significant differences were also noted in the types and locations of perimortem traumas. There is a significantly higher frequency of entrance gunshot wounds to the back of the head, suggestive of execution style murders, in the well series.