Utility of immunoassay in drug screening in skeletal tissues: sampling considerations in detection of ketamine exposure in femoral bone and bone marrow following acute administration using ELISA

Detection of ketamine exposure in skeletal tissues by automated enzyme-linked immunosorbent assay (ELISA) and gas chromatography with electron capture detection (GC-ECD) is described. Rats (n = 18) received 0, 15, 30, or 75 mg/kg ketamine hydrochloride acutely (i.p.), and were euthanized within 15 min or 1 h. Ketamine was extracted from ground femoral bone by methanolic incubation followed by liquid-liquid extraction (LLE), while marrow was homogenized in alkaline solution, and then underwent LLE. Extracts were analyzed by ELISA, and subsequently by GC-ECD following derivatization with trifluoroacetic acid anhydride. The effect of tissue type (i.e., diaphyseal bone vs. epiphyseal bone vs. bone marrow) on the immunoassay response was examined through determination of binary classification test sensitivity (S) and measurement of the relative decrease in absorbance (%DA, drug-positive tissues vs. drug-free controls) in each tissue type. The %DA varied significantly between different tissues examined under a given dose condition, and generally decreased in the order marrow > epiphyseal bone > diaphyseal bone, at all dose levels examined. Measured S values for marrow, epiphyseal bone, and diaphyseal bone were 100%, 77%, and 23%, respectively (75 mg/kg dose). These results suggest that the type of skeletal tissue sampled and position sampled within a given bone (diaphyses vs. epiphyses) are important parameters in drug screening of skeletal tissues.     

中国人四肢长骨骨性标志间各段长度与总长关系的分析

利用碎裂的长骨片段 ,计算长骨的总长。选择中国人长骨 5 0 0余例 ,取其骨性标志分段测量记录 ,将测得的数据输入计算机 ,用社会科学软件包 (SPSS)中全回归法进行统计学处理。在得出的 33个多元回归方程中 ,方程1、 9、 18、 2 6证明了骨性标志间各段长度与总长的正相关关系 ,其余 2 9个方程是在有选择的略去 1个变量和因长骨近端、远端或两端缺损使 1个或 1个以上变量缺失的情况下得出。根据碎裂长骨片段骨性标志间的长度测量值 ,应用多元回归方程 ,可计算出长骨的总长     

青少年骨关节X线片的骨龄研究

通过对11～16周岁女性及13～20周岁男性青少年左侧肩、肘、腕、髋、膝、踝关节拍摄X线片,进行了青少年的骨龄研究.根据骨骼生长发育的特点,提出了各大关节X线片判定骨龄的观察指标及分级标准,并使用SPSS软件包进行数据处理,建立了青少年利用6大关节骨骺愈合程度判定女性是否已满14周岁、男性是否已满16周岁或18周岁的判别分析方程.     

A Silver‐Stain Modification of Standard Histological Slide Preparation for Use in Anthropology Analyses

The traditional histology method typically employed by forensic anthropologists involves plastic embedding of undecalcified bone. The embedded sample is then cut by a diamond blade saw and ground to the required thickness of ~50–100 microns using a grinder. There are several limitations to this method: Cement lines may be blurred; depth‐of‐field artifacts may result from viewing thick sections; and medicolegal offices with limited budgets may not be able to invest in additional equipment or training for this method. A silver nitrate stain modification of the standard histology preparation technique of decalcified bone is presented. The benefits of this technique are that: Cement lines are viewed clearly; no depth‐of‐field artifacts are present; and because this is a modification of the standard technique used by histology laboratories typically employed by medicolegal offices, no additional equipment or training is required.     

A Computational Framework for Age‐at‐Death Estimation from the Skeleton: Surface and Outline Analysis of 3D Laser Scans of the Adult Pubic Symphysis

In forensic anthropology, age‐at‐death estimation typically requires the macroscopic assessment of the skeletal indicator and its association with a phase or score. High subjectivity and error are the recognized disadvantages of this approach, creating a need for alternative tools that enable the objective and mathematically robust assessment of true chronological age. We describe, here, three fully computational, quantitative shape analysis methods and a combinatory approach that make use of three‐dimensional laser scans of the pubic symphysis. We report a novel age‐related shape measure, focusing on the changes observed in the ventral margin curvature, and refine two former methods, whose measures capture the flatness of the symphyseal surface. We show how we can decrease age‐estimation error and improve prior results by combining these outline and surface measures in two multivariate regression models. The presented models produce objective age‐estimates that are comparable to current practices with root‐mean‐square‐errors between 13.7 and 16.5 years.     

MSCT及图像后处理在鼻骨骨折法医鉴定中的应用

目的探讨多层螺旋CT(MSCT)及图像后处理在鼻骨骨折法医学鉴定中的应用价值。方法采用MSCT对134例被鉴定人进行薄层扫描并行多平面重建(MPR)和表面遮盖成像(SSD)。比较MSCT与常规X线诊断结果。结果线型骨折55(41.04%)例,粉碎型骨折46例(34.33%),凹陷型骨折27例(20.15%),未见骨折6例(4.48%)。X线平片及CR片漏、误诊48例(35.82%)。MSCT正确诊断133例(99.25%)。两者诊断结果存在显著差异(χ2=45.0816,P<0.001)。结论MSCT及后处理图像应作为鼻骨骨折法医鉴定的主要依据。     

DNA analysis of the last Brazilian unknown soldier’s remains buried in Pistoia (Italy)

During World War II, many nations took part in the war. Among the supporters of the Alliance there was also Brazil. In August 1944, under the leadership of President Getúlio Vargas, Brazil declared war on Nazi Germany and took part in the Italian campaign by sending many troops to support the Allies in the Central Italy. Once the conflict was over, the deceased Brazilian soldiers were buried in Pistoia, a few kilometers from Florence. But only in 1960 the Brazilian government authorized the transfer of the dead soldiers to their homeland. Five years later, during the building of the Brazilian Military Votive Monument, still in the Pistoia cemetery, a last body was found but could not be identified: so he was buried as an “unknown soldier”. In December 2012, the Brazilian Embassy in Italy asked for performing forensic genetics analysis for identification purposes on the remains of this last unknown Brazilian soldier. After almost 70 years a complete short tandem repeats (STR) profile was obtained, useful for any relatives searching.Key points:

• Identification of the last Brazilian Unknown Soldier buried in Italy.
• DNA analysis on 70 years old skeletal remains.
• Brazilian soldier’s history during World War II.

     

Constancy and change in the U.S. age distribution of crime: A test of the “invariance hypothesis”

The recent controversy over the age relationship with criminal behavior can be traced to Hirschi and Gottfredson's (Am. J. Sociol. 89, 552–584, 1983) failure to define invariance. In this paper, I distinguish two types of invariance—parametric andmathematical form— that explains both the pattern of stability claimed by Hirschi and Gottfredson and the pattern of variability observed in more recent research. I then directly test for each type of invariance using age-specific arrest data for index offenses in the United States from 1952 to 1987. The results are largely consistent with recent research showing variability in the characteristics (i.e., parameters) of the age distribution of crime, but at the same time, the results provide evidence for a persistent, underlying mathematical form to the age distribution of crime, regardless of year or offense type.     

我国矿产资源利益分配制度刍议

改革开放以来,中国经济发展取得了举世瞩目的成就,然而在增长的奇迹背后却是资源耗竭和环境污染。从宏观层面而论,这是我国长期以来实行粗放型经济增长方式的负面结果,但同时需要认识到,它与我国矿产资源利益分配制度的缺陷有直接关系。现行矿产资源利益分配制度导致了一种不合理的利益格局,各个利益相关者之间存在着权责不一致、分配不合理、成本外部化等问题。为构建社会主义和谐社会,实现可持续发展,建立产权清晰、税费明确、分配合理、补偿到位的矿产资源利益分配制度势在必行。     

Population size or population density? An empirical examination of scale economies in South Australian local government, 2015/16

ABSTRACT

Advocates of amalgamation typically claim that substantial scale economies flow from municipal mergers, which generate larger local government entities by population size. We examined whether economies of scale exist in council outlays by analysing the expenditure of 68 South Australian local government areas using data from the 2015/16 financial year. However, given the correlation between population size and population density it is important to determine whether the influence of population size on expenditure is due to variations in population density. We find that when local government areas are stratified into subgroups on the basis of population density, the evidence of economies of scale largely evaporates. From a policy perspective, this suggests that in place of municipal mergers, policymakers should instead explore avenues for shared service arrangements in those functions which exhibit scale economies.