黄牛脑水通道蛋白4的表达及其对高原脑水肿的易感性

为了探讨黄牛对高原脑水肿的易感性,并为青藏高原养牛业提供参考,运用免疫组织化学SABC染色法并采用Image-Pro Plus 6.0软件,对成年黄牛大脑不同功能区水通道蛋白4(AQP4)的表达及分布特征进行了研究。结果显示,黄牛不同功能区脑组织中AQP4表达面积(S)和积分光密度(IOD)值的大小顺序为S扣带回>S中央前回>S丘脑>S尾状核,IOD扣带回>IOD中央前回>IOD丘脑>IOD尾状核,且扣带回和中央前回的S和IOD值均显著高于丘脑和尾状核(P<0.01)。结果证实,成年黄牛脑不同功能区、同功能区不同层及同功能区同层不同类型细胞对水的通透性及代谢功能存在较大差异,对脑水肿的易感性是不同的。     

金黄色葡萄球菌黏附素FnBPB-ClFA共表达质粒的构建与原核表达

采用PCR方法对编码金黄色葡萄球菌纤黏蛋白B的D区和纤黏蛋白原凝集素A的A区基因片段进行了特异性扩增,并通过重叠延伸PCR扩增了FnBPB-ClFA串联基因,构建了克隆质粒pMD19-FnB-PB-ClFA。将该基因片段定向插入到原核表达载体pET-32a(+)中,构建了表达质粒pET-FnBPB-ClFA,并将其转入宿主菌BL21(DE3)。SDS-PAGE分析表明,在1mmol/L IPTG诱导下,在51ku处出现了与目的蛋白一致的外源蛋白带。Western-blot分析表明,所表达的蛋白与目的蛋白一致。上述结果表明,该融合基因在原核细胞中成功表达。     

General and maladaptive traits and anger in a combined forensic psychiatric and general population sample

The present study examines the associations between anger and general and maladaptive traits in a combined forensic psychiatric and community sample of men. Thirty-four male forensic psychiatric patients and 219 men from the general population completed the Novaco Anger Scale (NAS; Novaco, 1994), the NEO-PI-R (Costa & McCrae, 1992), the ADP-IV (Schotte & De Doncker, 1994) and the VKP (Duijsens, Haringsma, & EurelingsBontekoe, 1999) at two measurement occasions with a one-year interval. The results of a series of multiple regression analyses showed that general and maladaptive traits accounted for a substantial amount of variance in anger scores. Neuroticism, Agreeableness and Externalizing traits were most prominent in the prediction of anger, while Conscientiousness was acting as a suppressor variable in the regression models. Strengths and limitations of the present study and implications for further research and clinical practice are discussed.     

城市化对经济增长的作用研究

城市化与经济增长相互作用对于社会发展的重要性不言而喻,自改革开放以来,我国城市化具有显著递增的规模效应,对经济增长有着良好的促进作用。     

The Role of Peer Contacts in the Relationship Between Parental Knowledge and Adolescents' Externalizing Behaviors: A Latent Growth Curve Modeling Approach

The purpose of the present study was to examine the direct and indirect effects (through peer contacts) of parental knowledge on adolescents’ delinquent and aggressive problem behavior, using latent growth curve modeling. A sample of 457 13- to 14-year old adolescents at first measurement wave (M=13.27; SD=0.45 years) filled out questionnaires about their parents, peers, and problem behavior three times with 1-year intervals in between. Regarding initial levels of behavior, both direct and indirect effects of parental knowledge were found on aggressive as well as on delinquent behavior. When the rate of change in behaviors was considered, only direct effects were found for both types of problem behavior, whereas indirect effects were absent. Gender differences were also found, with stronger effects of parenting on both aggressive and delinquent problem behavior for boys and stronger effects of peer contacts on aggressive behavior for girls. The present study shows that different behaviors of the externalizing spectrum have different trajectories and diverse relations with parenting and should not be treated as identical.

Review of pathologic findings in remnant hearts following valve donation

The failure of medical examiners/coroners (ME/C) to allow heart valve donation is a major problem encountered by tissue agencies. Even though many ME/C favor tissue donation they remain responsible for determination of cause and manner of death. In 2001, the Jesse E. Edwards Registry of Cardiovascular Disease was approached by one of the nation's largest tissue procurement agencies (The American Red Cross--ARC) for the purpose of performing cardiovascular pathologic examinations following valve donation. The affiliation existed from October 2001 to January 2005. This study was undertaken to review all 593 postvalve recovery heart remnants received during that time period to tabulate the abnormalities identified and to determine whether donation interfered with the determination of cause of death. For each case, a preliminary cause of death was provided by the ARC. The decedent's body height and weight were also provided. Using the preliminary cause of death, the 593 cases were divided into natural and nonnatural manner of death groups. This division of the cases resulted in 106 cases placed in the natural manner of death group and 487 cases in the nonnatural manner of death group. For each case, all cardiac findings including significant conditions, additional findings, incidental findings, and congenital abnormalities were tabulated. Within the natural manner of death group, 15 cases had a noncardiac cause of death and 91 cases had a cause of death suspected to be cardiac related. In the 91 cases, a total of 132 significant cardiac findings were identified and there were six structurally normal hearts including two infants. In the nonnatural manner of death group, 214 significant cardiac findings were identified and 222 cases had a structurally normal heart. In both natural and nonnatural groups, the most common cardiac abnormality was atherosclerotic coronary artery disease. Other frequently encountered conditions were also identified including 11 cases with acute angle of origin of a coronary artery (five cases natural group; six cases nonnatural group). An important feature of this review was the recognition of potentially inheritable conditions that were diagnosed in both natural and nonnatural manner of death groups. There were three cases of hypertrophic cardiomyopathy (one natural; two nonnatural), three cases of arrhythmogenic right ventricular cardiomyopathy (one natural; two nonnatural), and one case of mitral valve prolapse (natural). In reviewing these cases, we did not feel that valve donation severely impaired cardiac pathologic examination. The benefits of cardiovascular pathologic examination by a cardiac pathologist include the identification of significant and incidental findings and recognition of potentially inheritable conditions.     

Comparative Analysis of the Effects of Heat on the PCR‐Amplification of Various Sized DNA Fragments Extracted from Sus Scrofa Molars*

Abstract: This study examined the effects of heat on the amplification of DNA from the dental pulp of Sus scrofa molars and investigated the protection afforded to the pulp tissue by the dental enamel, alveolar process, and soft tissue of the head. Segments of defleshed maxilla and mandible encasing the first molar (n = 60) were subject to a range of temperatures for 15 min. Dental pulps were retrieved. Amplifications using three‐primer and four‐primer multiplexes showed no degradation of the largest fragment following exposure to 450°C. Amplifications in the three‐primer multiplex (283 bp) were successful following exposure to 525°C in maxillary samples only. This study revealed the enamel density of maxillary molars to be greater than mandibular molars in Sus scrofa. Following incineration of intact heads for 15 min (n = 10) and 1 h (n = 4) at an average temperature of 625°C, amplifications of the largest fragment (450 bp) were successful from both maxillary and mandibular teeth.     

我国环境规制与经济增长关系的区域差异分析——基于东、中、西部面板数据的实证研究

根据1998—2006年中国30个省区的面板数据,运用相关的计量经济学方法,对我国东、中、西部地区环境规制与经济增长的关系进行实证研究的结果表明,我国环境规制水平与经济增长的关系呈现出明显的区域差异性。长期内,东、中、西部地区环境规制水平与经济增长均互为因果关系,短期内东部地区环境规制水平对经济增长影响显著,中部地区两者之间关系不显著,而西部地区经济增长在一定程度上推动了环境规制水平提升。据此应当采取相应的政策措施,以促进中国各区域环境规制与经济增长的良性互动和协调发展。     

浅议超大城市经济增长模式

超大城市经济增长模式的内涵应该包括经济增长驱动力、资源配置方式、资源利用效率和环境支撑力以及超大城市与周边地区关系四个部分。促使超大城市经济增长模式发展变化的主要影响因素可以概括为三个方面,历史沿革、发展现状和功能定位。而对于超大城市经济增长模式的评价体系,主要来自于三个视角：包括经济增长的实效、经济增长的潜力以及经济增长的可持续性。     

论不当得利冲突规范中的“发生地法主义”

单纯的不当得利发生地法主义,即以不当得利发生地为唯一空间连结因素的双边冲突规范,就立法沿革而言,只是个过时的立法主流;就立法模式而言,此项立法主义属传统冲突规范范畴,故带有传统规范自身无法克服的僵硬封闭之缺陷,需要借助共同属人法、法院地法、原因关系准据法来软化;而就其内涵而言,“不当得利发生地”这样的表达不够准确,其指向的连结点可以是损害发生地、利益获得地和原因事实发生地,而原因事实发生地最为合理。不当得利冲突规则至今在我国尚属立法空白,因此厘清不当得利发生地之内涵,并软化该连结点后,此项主义可为我国立法所用。