Extraction of high quality DNA from biological materials and calcified tissues

Calcified tissues, such as bone and tooth, and some other sample types, such as those containing adhesive, present a challenge to standard extraction protocols. We have developed a lysis reagent, BTA™ lysis buffer, which is designed for use with PrepFiler™ Kit reagents. The BTA™ lysis buffer disrupts calcified tissue matrices and achieves effective extraction of DNA from pulverized bone and tooth samples. In addition, the BTA™ lysis buffer mildly but efficiently extracts DNA from challenging substrates like tape, chewing gum, and cigarette butts and, as with bone and tooth, DNA from these lysates is purified using established PrepFiler™ reagent extraction protocols.We successfully extracted DNA from powdered human bone samples, chewed gum and smoked cigarettes using BTA™ lysis buffer. Extraction yields for bone, gum and cigarette samples tested were consistent and reproducible. This extraction method efficiently removed potential PCR inhibitors from all samples tested, and C_T values for the internal PCR control of Quantifiler^® Human DNA Quantification Kit were consistent and within the normal range. The DNA extracted from these samples also provided conclusive profiles that were free of PCR artifacts when amplified using the AmpF?STR^® Identifiler^® PCR Amplification Kit. The protocol is easily adapted for automation.     

暴力犯罪后“捎带财物行为”的定性及理性分析

行为人在实施侵犯人身暴力犯罪后“捎带财物行为”的定性,应当具体分析暴力犯罪后“捎带财物行为”性质,即判断先前侵犯人身的暴力犯罪给被害人造成的状态,接着判断先前暴力行为是否形成新的“威胁”以及是否被“捎带财物行为”实施时所利用,最终作出合理的结论。     

中部地区某县104起投毒案件分析

通过对104起投毒案件分析,发现投毒杀人仍是投毒犯罪的一个主要目的;投毒案件既有一定的季节性也表现出一定的地域性;所投毒物种类分布较广,但农药和鼠药占多数;所投毒物具有鲜明的时代性;投毒位置、对象和手段有一定的选择性等规律。     

农村社区警务建设存在的问题及对策分析

农村社区警务的实施对于建设社会主义新农村,保证全社会的安定有序有着重要的意义。但现在农村社区警务存在着很多问题：一是部分民警对社区警务工作重视不够：二是公安机关用于农村社区警务的经费和警力保障不足;三是警民关系不够和谐,农村社区警务工作没有形成警民合力。因此,农村社区警务建设的重点是：一、完善法律法规;二、警力资源的科学配置;三、民警素质的有效增强。     

对实用艺术作品著作权保护之法律探析——完善我国《著作权法》立法建议

我国《著作权法》及《著作权法实施条例》中,对实用艺术作品均没有任何明确的规定,实用艺术作品的法律保护至今仍为空白。我国应结合国际公约、条约和国外一些国家的法律规定,在《著作权法》中应明确规定对实用艺术作品的著作权保护的具体措施。     

人民民主专政理论的哲学分析及当代启示

人民民主专政理论是毛泽东思想的重要组成部分,包含着丰富的哲学思想。关于这一理论的哲学分析,对当前中国特色社会主义建设的启示是:党必须推动中国经济社会的全面发展,必须始终把发展作为党执政兴国的第一要务,必须始终坚持以人为本的执政理念,必须用发展着的马克思主义指导中国新的实践。     

师专学生心理健康状况的调查分析及对策

对1986名师范专科学校的新生的心理健康状况调查表明,有12.34%的学生存在不同程度的心理症状和心理问题,有50.14%的学生存在人际关系不协调、新环境不适应等问题。造成大学生心理健康状况欠佳的原因是多方面的,学校有必要进一步加强学生的心理卫生教育。     

Science Parks’ tenants versus out-of-Park firms: who innovates more? A duration model

We study the successfulness of Science Parks (SPs) as seedbeds of innovation. We investigate whether SPs enhance the innovative output of their tenants and if tenants outperform comparable outside-SPs firms. We rely on original matching datasets regarding in- and out-SP Finnish firms and their patenting activity over 1970–2002. We estimate and compare the ‘before-versus-after’ hazard rates of patenting of both samples. The results suggest that, given the existence of a common tendency to slow down the pace at which all firms patent during their life cycle, Park tenants exhibit a comparatively better performance. Results are robust to various model specifications and to Wald tests performed over the pooled samples.

Identification of acepromazine in hair: an illustration of the difficulties encountered in investigating drug-facilitated crimes

After a drug-facilitated sexual assault (DFSA), a woman was found in a drowsy state at home. She remembered having drunk an unknown beverage by the accused. Blood samples (collected 8 hours after the DFSA), two glasses, and a teaspoon seized by the police were analyzed. Acepromazine, a phenothiazine tranquilizer used in human and veterinary medicine, was detected in the residue of one of the glasses. In spite of acepromazine absence in the victim's blood, the possible use of acepromazine in the DFSA was reported to the police. Two weeks later, a suspect admitted having orally administered acepromazine to the victim. Using a liquid chromatography-tandem mass spectrometry method, this compound was subsequently detected (31 pg/mg) in a sample of the victim's hair collected a month and a half after the DFSA. A potential short elimination half-life in humans and/or the well-known in vitro degradation of acepromazine could explain the negative blood result. DFSA toxicological investigations are challenging and can be complicated when a rather unusual substance is concerned. In particular, special care should be taken when interpreting the results, taking into account elimination and/or instability data, when available.