浅析倾诉之后的矛盾

目前,"隐私倾诉"、"情感实录"等各类倾诉谈话类栏目比比皆是,有的还将倾诉故事集结成册成书且在市场上热销。但是作为当事人在倾诉之后的矛盾心情,却很少有人再去探究。我们有必要针对人们倾诉之后的矛盾,了解相关的原因以及倾诉之后的心理、行为,进而分析其倾诉的必要性,从而进行有效的引导和疏通。     

牛分支杆菌MPT83基因的表达及重组蛋白的纯化

从牛分支杆菌培养物中抽提总DNA,扩增了MPT83基因,并克隆入pMD 18-T Simple Vector,将测序正确的目的基因插入表达载体pET-32a( )中,转化BL21(DE3)宿主菌,IPTG诱导表达,用亲和层析方法对表达蛋白进行了纯化。经测序,构建的克隆质粒pMD-MPT83与Gen- Bank中的序列一致;构建的表达质粒pET-MPT83经PCR和BamHⅠ HindⅢ双酶切鉴定构建正确;经SDS-PAGE分析,在约42 ku处出现新的蛋白条带,4 h后表达量即达到高峰;Western- blotting分析表明,融合蛋白能够被牛分支杆菌阳性血清所识别;纯化的表达蛋白经SDS-PAGE电泳,出现清晰的单一条带。表明MPT83基因原核表达载体构建成功。     

民法动机错误论考——从类型论到要件论之嬗变

严格区分动机错误与表示错误、原则上将动机排斥于法律评价之外,唯有表示于外的动机始受保护的传统的“类型论”观点日益露出其破绽,将动机错误与表示错误一元化并构造统一法律要件的“要件论”思想渐次成为今日“错误论”之主流,而统一的“错误要件”如何最大限度地保持合理性,仍在继续的探索与求证之中。     

鸡传染性腔上囊病病毒Gt株全基因组序列分析及真核表达载体的构建

采用一步法长距离RT-PCR克隆了IBDV Gt株全基因组,对其核苷酸和氨基酸序列进行了系统分析。在IBDV Gt株全基因组中引入分子标签,且在基因组两端分别引入锤头状核酶结构(HamRz)和丁肝病毒核酶结构(HdvRz)。将带有分子标签和核酶结构的IBDV基因组插入载体pCAGGS的β肌动蛋白启动子下游,构建了IBDV真核表达载体pCAGGmGtAHRT和pCAG-GmGtBHRT,为IBDV新型高效拯救平台的构建及基于此的病毒基因功能奠定了基础。     

对外汉语教学中文学作品的阅读的教学思考

本文从文学创作的特点给以语言教学为宗旨的文学课带来的阅读障碍问题;文 化冲突问题;语言得体表达问题入手,分析了其问题出现的原因及解决的相应手段。     

猪囊虫抗原基因TS21的真核表达

构建了猪囊虫抗原基因TS2 1的VR10 2 0真核表达载体 ,以菌落原位杂交法筛选获得正确插入的重组质粒 ,用RNA印迹 (Northernblotting)和ELISA检测插入片段的转录及其翻译表达产物。结果 ,VTS2 1在真核细胞中能够转录TS2 1基因的mRNA ;兔抗猪囊虫超免疫血清可识别其蛋白表达产物 ,表明VTS2 1能正确表达猪囊虫抗原蛋白     

口蹄疫病毒多基因片段的克隆及其杆状病毒表达载体的构建

根据定点突变原理获得了包含口蹄疫病毒P1、2A、3C、3D及部分 2B编码区的目的基因片段 ,经SpeⅠ和HindⅢ双酶切后 ,与经相同方法处理的杆状病毒转移载体 pFastBacHT连接 ,得到了重组质粒 pFB P12X3C3D。经酶切和测序鉴定后 ,将其转化入含穿梭载体Bacmid的感受态细胞DH10Bac ,经抗性及蓝白斑筛选 ,得到了含P12X3C3D多基因的重组穿梭载体 ,将其命名为Bacmid P12X3C3D ;提取其DNA并转染Sf9昆虫细胞 ,最后获得含口蹄疫病毒P12X3C3D多基因的重组杆状病毒。     

伪狂犬病病毒闽A株糖蛋白gD基因去信号肽片段在大肠埃希氏菌中的表达

应用PCR方法从包含伪狂犬病病毒(PRV)Fa株糖蛋白gD基因的重组质粒pB13中扩增出糖蛋白gD基因去信号肽片段,将其克隆到大肠埃希氏菌表达载体pThiohisA中。测序结果表明,糖蛋白gD基因去信号肽片段长1 155 bp,与PRV Fa株糖蛋白gD基因完全一致。经1mmol/L IPTG诱导后,重组质粒pThiohisA-gD在大肠埃希氏菌XL1-blue、Top10、DE3和DH5α中均得到表达,其中在宿主菌XL1-blue中表达量最高。Western-blotting结果显示,大肠埃希氏菌XL1-blue表达的糖蛋白gD具有免疫原性。     

司法鉴定领域分类和能力范围表述的国内外比较研究

对司法鉴定领域开展科学、有效的分类,进行统一规范的能力范围表述,有助于建立完整的司法鉴定标准化体系,能为司法鉴定机构合理规划组织结构、准确定位发展方向提供指引,也能为司法鉴定行业管理部门的规范化管理提供帮助。对该领域分类和能力表述开展国内外比较分析,是实现上述目标的基础性研究。目前我国在司法鉴定领域的分类方法尚未统一,与国外法庭科学认可分类方法相比,尚有部分项目未纳入分类范围之内;国内能力范围表述在目的、方式、详细程度上与国外相比亦有不同。本文对国内外行业管理组织和国际权威认可机构司法鉴定领域分类及能力范围表述进行了比较分析,研究解析其内容及差异,并提出了制定司法鉴定领域分类国家标准、扩展司法鉴定领域分类内容、适时调整司法鉴定能力范围表述内容等建议。     

The North Korean abduction issue: emotions,securitisation and the reconstruction of Japanese identity from ‘aggressor’ to ‘victim’ and from ‘pacifist’ to ‘normal’

Abstract

After Kim Jong-il's confession in 2002 that North Korean agents had abducted thirteen Japanese citizens in the 1970s and 1980s, North Korea has become the most detested country in Japan, and the normalisation of bilateral relations has been put on the back burner. The abduction issue has taken precedence in Japan even over North Korea's development of nuclear weapons and long-range missiles. It has also grossly overshadowed the atrocities for which Imperial Japan was responsible in the 20th century. Why has there been such strong emphasis on an issue that could be disregarded as comparatively ‘less important’? This article understands the ascendency of the abduction issue as the epitome of an identity shift under way in Japan – from the identity of a curiously ‘peaceful’ and inherently ‘abnormal’ state, to that of a more ‘normal’ one. The differentiation of North Korea as ‘abnormal’ emphasises Japan's own (claim to) ‘normality’. Indeed, by incarnating the perils of Japan's own ‘pacifist’ ‘abnormality’, which has been so central to the collective sense of Japanese ‘Self’ in the post-war period, the abduction issue has become a very emotional argument for Japan's ‘normalisation’ in security and defence terms. The transformation from ‘abnormal’ to ‘normal’ is further enabled by Japan trading places with North Korea in the discourse, so that Japan is defined as ‘victim’ (rather than former aggressor) and North Korea as ‘aggressor’ (rather than former victim). What is at stake here is the question whether Japan is ‘normalising’ or ‘remilitarising’, and the role of the abduction issue discourse in enabling such foreign and security policy change.