小鼠骨骼肌细胞核DNA降解与死亡时间的关系

目的　监测小鼠死后骨骼肌核内DNA降解的情况 ,探讨死后细胞核DNA降解的一般规律。方法　建立小鼠死亡模型 ,在死后 72h内 ,以 12h的间隔取骨骼肌样本进行单细胞凝胶电泳 ,在荧光显微镜下测量彗星图像 ,并作统计学分析。结果　机体死后 ,骨骼肌细胞在电泳图像上出现了明显的彗星形拖尾 ,L/W比值随死亡时间而逐渐增大 ,二者呈一定的线性关系。结论　单细胞凝胶电泳技术可以应用于早期死亡时间推断。     

Bounding the number of contributors to mixed DNA stains

We derive a simple inequality for the probability of observing a given DNA profile when assuming a fixed number of unknown persons have contributed to the mixed stain. We then show how this inequality can be used to obtain an upper bound for the number of unknown contributors needed to be considered.     

DNA指纹的个体特异性和细胞稳定性研究

人类基因组的许多高度可变区域能够与小卫星探针杂交,而同时被检测出来,小卫星探针由串联重复的"核心"顺序构成.采用Southern印迹杂交获得的DNA指纹图包含多条高度可变的DNA片段杂交带,这些带主体细胞和生殖细胞中都表现出稳定性,而且安全具有个体特异性.本文用小卫星探针33.15,调查了北京地区的60名无关个体,     

认知询问技术原理及其应用

认知询问技术是心理学与侦查实践相结合的产物。认知询问技术建立在有效询问的条件上 ,其心理原理是记忆与沟通 ;拥有一个核心程序 ,强调证人中心角色。其核心程序就是引导记忆提取 ,即侦查人员必须帮助与鼓励证人接近或进入已经储存在长时记忆中的与案件有关的信息。强调证人中心角色是指将证人摆在询问的核心位置。     

浅议调解劳动争议案件中谈话的技巧

在调解劳动争议案件过程中,掌握谈话技巧是十分重要的。应当根据争议双方在调解过程中的种种心态,把握好个别谈话应注意的问题和方法,从而达到调解劳动争议的目的。     

合理开发利用贵州少数民族传统体育推动贵州旅游业的发展

贵州少数民族传统体育融音乐、舞蹈、体育为一体,是民族文化的表现形式,是重要的民族文化旅游资源。从旅游业的角度,本文从少数民族体育具有的开发利用价值、开发利用的必要性,论述了贵州少数民族传统体育的开发利用对贵州旅游业发展的积极推动作用,并分析了对其开发利用必须坚持的原则和措施。     

市场经济法制构建的障碍与完善

市场经济模式在我国已初步形成,进一步发展和完善我国社会主义市场经济,必须加强市场经济的法制建设。对市 场经济法制建设中存在的主要障碍,建议从法制建设的主导思想、理论研究及具体方法入手进行解决,并提出构建市场经济法制 完善之思路。     

浅谈擒敌技术训练的组织与实施

擒敌技术训练的组织与实施包括训练准备、训练实施与考核验收几个步骤。训练准备有编写教案、准备场地器材、培训示范班等内容;训练实施包括作业的准备、实施、小结讲评与作业讲评;考核验收要严,严格标准。     

对刑事司法程序视野下DNA证据的探讨

DNA证据在刑事司法程序中有着独特的自身价值,但这并不意味着DNA证据就是完美无缺的“证据之王”。在当前我国的刑事司法领域中,关于DNA的鉴定和证据适用等方面还存在着诸多的问题。因此,我国应在DNA证据采信等环节上建立和完善相应规则,克服对DNA证据的盲目轻信。     

Investigation on the genetic-inconsistent paternity cases using the MiSeq FGx system

Mutations might challenge the paternity index calculation in forensic identification. While many studies have focussed on the autosomal short tandem repeats (A-STR), the mutation status of sex chromosomes and single nucleotide polymorphism (SNP) remain blank. Next generation sequencing (NGS), known as high throughput and large sequence polymorphism, is a promising tool for forensic genetics. To describe the mutation landscapes in the paternity cases with genetic inconsistencies, a total of 63 parentage confirmed paternity cases contained at least one mismatched locus have been collected. The mutations were subsequently evaluated using Verogen’s MPS ForenSeq^TM DNA Signature Kit and a microsatellite instability (MSI) detection kit. The result showed 98.41% (62/63) of the cases had no additional autosomal mutations even when the number of A-STRs increased to 27. As for the sex chromosomes, about 11.11% (7/63) of the cases exhibited either X-STR or Y-STR mutations. D2S1338, FGA and Penta E were the most frequent altered STRs, which suggested they might be the mutation hotspots. In addition, a male with sex chromosome abnormality was observed accidently, whose genotype might be 47, XXY, rather than MSI. Nearly 56.90% of the STR loci possessed isoalleles, which might result in higher STR polymorphisms. No Mendelian incompatibility was detected among the SNP markers, which indicated that SNP was a more reliable genetic marker in the genetic-inconsistent paternity cases.Supplemental data for this article is available online at https://doi.org/10.1080/20961790.2021.2009631 .