浅议调解劳动争议案件中谈话的技巧

在调解劳动争议案件过程中,掌握谈话技巧是十分重要的。应当根据争议双方在调解过程中的种种心态,把握好个别谈话应注意的问题和方法,从而达到调解劳动争议的目的。     

合理开发利用贵州少数民族传统体育推动贵州旅游业的发展

贵州少数民族传统体育融音乐、舞蹈、体育为一体,是民族文化的表现形式,是重要的民族文化旅游资源。从旅游业的角度,本文从少数民族体育具有的开发利用价值、开发利用的必要性,论述了贵州少数民族传统体育的开发利用对贵州旅游业发展的积极推动作用,并分析了对其开发利用必须坚持的原则和措施。     

刑事技术在缉毒中的应用

在毒品案件的缉查中 ,我们一方面要注意通过侦查渠道获取直接证据 ,另一方面我们一定要重视运用刑事技术手段 ,收集同案件有关的各类物证、书证、视听资料、鉴定结论等其他间接证据 ,并使之相互印证 ,共同构成一个完整的证据体系 ,切实做到案件实事清楚 ,证据确凿充分 ,为毒品查缉工作发挥刑事技术的作用     

市场经济法制构建的障碍与完善

市场经济模式在我国已初步形成,进一步发展和完善我国社会主义市场经济,必须加强市场经济的法制建设。对市 场经济法制建设中存在的主要障碍,建议从法制建设的主导思想、理论研究及具体方法入手进行解决,并提出构建市场经济法制 完善之思路。     

浅谈擒敌技术训练的组织与实施

擒敌技术训练的组织与实施包括训练准备、训练实施与考核验收几个步骤。训练准备有编写教案、准备场地器材、培训示范班等内容;训练实施包括作业的准备、实施、小结讲评与作业讲评;考核验收要严,严格标准。     

Touch DNA collection from improvised explosive devices: A comprehensive study of swabs and moistening agents

Improvised explosive devices (IEDs) are used in devastating terrorist attacks worldwide and daily in Thailand. Touch DNA deposited during IED assembly are subjected to intense heat and pressure, resulting in rare events of usable DNA profiles obtained from real casework. No study has simultaneously evaluated both swab brands and moistening agents for touch DNA collection from substrates encountered in IED evidence. In this study, we investigated the effects of swab brands and moistening agents on DNA collection from adhesive tape, a common IED substrate. A full factorial design using four cotton swab brands (two forensic and two medical cotton swabs) and six moistening agents (DNA-free water, phosphate-buffered saline, ethanol, sodium dodecyl sulfate, isopropanol, and lysis buffer) was employed (24 total combinations). Using buffy coats, we found that DNA recovery depended on both swab brands and moistening agents (p < 0.05). The optimal method recovered significantly higher DNA amount from real IED cases compared to the standard Royal Thai Police method. Percentages of high partial profiles also increased. Our results changed the standard operating protocol of the Thai police. Other commonly found substrates from IED cases are being investigated to maximize the evidential value obtained from touch DNA.     

Estimating Individual Contributions to Complex DNA SNP Mixtures.,

High‐throughput sequencing (HTS) of large panels of single nucleotide polymorphisms (SNPs) provides an alternative or complimentary approach to short tandem repeats (STRs) panels for the analysis of complex DNA mixture forensic samples. For STRs, methods to estimate individual contribution concentrations compare capillary electrophoresis peak heights, peak areas, or HTS allele read counts within a mixture. This article introduces three approaches (mean, median, and slope methods) for estimating individual DNA contributions to forensic mixtures for HTS/massively parallel sequencing (MPS) SNP panels. For SNPs, the major:minor allele ratios or counts, unique to each contributor, were compared to estimate contributor proportion within the mixture using the mean, median, and slope intercept for these alleles. The estimates for these three methods were typically within 5% of planned experimental contributions for defined mixtures.     

The CSI Effect and the impact of DNA evidence on mock jurors and jury deliberations

ABSTRACT

The present research examined the CSI Effect and the impact of DNA evidence on mock jurors and jury deliberations using a 3 (Crime Drama Viewing: low, moderate, high)?×?3 (Evidence: DNA innocent, DNA guilty, no DNA control) design. A sample of 178 jury-eligible college students read a case of breaking and entering. Pre-deliberation, some support for a CSI Effect was found with high viewers’ extent of guilt ratings significantly lower than moderate and low viewers’ in the no DNA control and the DNA innocent conditions. This effect was not present for verdicts. Contrary to a CSI Effect, crime drama viewing was not related to guilt judgments with incriminating DNA evidence. A content analysis of comments made during deliberations found little support for the CSI Effect entering the jury room. Specifically, CSI Effect predictions were not supported when examining the discussion of DNA evidence, expressing DNA opinions, or mentioning missing evidence. Overall, the limited CSI Effect found for individuals was attenuated during deliberation. The alarm raised over a possible CSI Effect influencing jury decision making may be unwarranted.     

口蹄疫病毒O型和A型交互反应性单克隆抗体的筛选与鉴定

不同血清型口蹄疫病毒(FMDV)之间不能产生交叉免疫保护,但是血清学的交叉反应确实存在。本研究旨在建立一种筛选型间交互反应性抗体的方法,以期为解析FMDV血清型间保守的抗原结构奠定基础。本方法主要依据单个B细胞抗体技术,首先采用两种不同的荧光染料FluoProbes 647H和Pacific Blue分别标记O型与A型FMDV 146S灭活抗原,然后利用流式细胞分选技术,从免疫牛外周血单个核细胞(PBMCs)中分选出结合两种抗原的特异性B细胞,通过单个B细胞抗体基因测定,获得Ig G抗体重链与轻链可变区编码序列,并将可变区基因插入含有牛IgG抗体恒定区的pcDNA3.4载体中,构建IgG抗体的重链与轻链表达质粒。将重链与轻链表达质粒共转染中国仓鼠卵巢悬浮细胞(CHO-S)进行完整抗体表达。结果显示,通过该方法获得了5株FMDV特异性单克隆抗体,其中4株(H64、R82、I16、R29)经间接免疫荧光(IFA)检测都可特异性识别O和A型FMDV;ELISA结果显示,H64、R82、I16与O、A型抗原均具有较好的亲合力;Western-blot结果显示,I16可特异性结合O、A型FMDV结构蛋白VP2中的线性表位,说明在衣壳蛋白VP2上存在血清型间保守的抗原表位,通过本研究进一步加深了对FMDV抗原结构的认识。     

不同消毒方式对现场DNA检出影响研究

目的 探讨新型冠状病毒敏感的消毒方式对于常见现场生物物证如血、脱落细胞的检出影响,探索在疫情期间现场勘查、实验室检验及尸体解剖前后的合理消毒方式.方法 采用《消毒剂使用指南》推荐的几种不同浓度化学消毒剂和紫外线照射等方式,对常量的血、口腔拭子和稀释的血、唾液斑进行作用后的样本DNA检出情况进行对比.结果 常用消毒方式对...