农民工认同社会主义核心价值观的思考

农民工作为中国工人阶级的重要组成部分,其对社会主义核心价值观的认同非常重要.认同的内容包括农民工对中国特色社会主义社会政治的认同、改变和提升农民工的物质生活条件、社会保障内容和丰富农民工的精神文化生活等内容.农民工认同社会主义核心价值观的过程是“外化”过程和“内化”过程的统一.     

马流感病毒NP单克隆抗体的制备及特性鉴定

利用原核表达的马流感病毒(EIV)核蛋白(NP)为免疫原,经腹腔接种4周龄BALB/c小鼠,取其脾细胞与SP2/0骨髓瘤细胞进行融合,经4次有限稀释法克隆和间接ELISA法筛选,获得了3株稳定分泌单抗的杂交瘤细胞株2G11、3E10和4A1,并对它们进行了亚类鉴定、间接ELISA检测和特性鉴定。结果显示,单抗2G11为IgG1亚型,3E10为IgG2a亚型,4A1为IgM亚型,轻链均为κ链。单抗2G11、3E10和4A1的细胞上清液及腹水效价分别为1∶640、1∶640、1∶320和1∶409 600、1∶204 800、0。这3株单抗均能特异性识别EIV NP重组蛋白,并且能够与天然EIV结合;获得的3株单抗与马动脉炎病毒、马疱疹病毒1型、马疱疹病毒4型、马乙型脑炎病毒均不发生交叉反应。亲和力试验结果显示,单抗2G11和3E10与EIV的亲和力常数分别为4.39×106 M-1和2.20×106 M-1。     

大熊猫源停乳链球菌类马亚种的分离与鉴定

为研究引起大熊猫脚掌化脓感染的原因,有效防治该病,对大熊猫感染部位的浓汁进行了细菌学检查及分离,对分离菌进行形态特征、理化特性鉴定、兰氏分群以及16S rDNA序列的分子鉴定,并构建系统发育树;对分离菌进行健康小鼠的人工感染试验。结果分离到5株细菌,根据5株分离菌的形态特征、理化特性及兰氏分群结果,结合16S rDNA序列测定与系统发育分析结果,判定为链球菌属的停乳链球菌类马亚种。该分离菌对供试健康小鼠有较强的致病作用。证实分离菌即为引起大熊猫脚掌化脓感染的病原菌。     

猪伪狂犬病病毒GZ-Z1株的分离鉴定

为获得1株猪伪狂犬病病毒贵州分离株,将贵阳市某猪场送检的流产胎儿病料经一系列处理后接种Vero细胞,并对分离毒株分别进行了病毒理化特性、电镜形态观察、中和试验、动物接种试验、核酸检测等鉴定。当盲传至第3代时,出现细胞变圆变亮、脱落、呈拉网状特征的细胞病变;分离毒不耐酸和热,对5-碘脱氧尿核苷和有机溶剂氯仿敏感,能被伪狂犬病病毒标准阳性血清中和;透射电镜下可见位于囊泡中呈近圆形、有囊膜、150～180 nm大小的成熟病毒粒子,在胞核中可见衣壳呈晶格状排列的病毒包涵体以及空心、实心2种无囊膜的病毒粒子;将分离毒株接种家兔,能引起家兔奇痒、麻痹死亡;PCR扩增产物经测序证明系PRV gD基因的部分序列。结果表明,成功分离获得了1株猪伪狂犬病病毒贵州株。     

DNA条形码技术在兽医寄生虫学研究中的应用

阐述了DNA条形码的概念、DNA条形码基因的筛选方法、DNA条形码的优势与劣势和分析方法,概述了近几年DNA条形码在兽医寄生虫方面的应用情况,旨在引起广大读者对该技术的关注。     

Identification of forensically important sarcophagid flies (Diptera: Sarcophagidae) in China, based on COI and 16S rDNA gene sequences

Insects attracted to cadavers may provide important indications of the postmortem interval (PMI). However, use of the flesh flies (Diptera: Sarcophagidae) for PMI estimation is limited as the species are often not morphologically distinct, especially as immatures. In this study, 23 forensically important flesh flies were collected from 13 locations in 10 Chinese provinces. Then, a 278-bp segment of the cytochrome oxidase subunits one (COI) gene and a 289-bp segment of the 16S rDNA gene of all specimens were successfully sequenced. Phylogenetic analysis of the sequenced segments showed that all sarcophagid specimens were properly assigned into four species (Boerttcherisca peregrina [Robineau-Desvoidy, 1830], Helicophagella melanura [Meigen, 1826], Parasarcophaga albiceps [Meigen, 1826], and Parasarcophaga dux [Thompson, 1869]) with relatively strong supporting values, thus indicating that the COI and 16S rDNA regions are suitable for identification of sarcophagid species. The difference between intraspecific threshold and interspecific divergence confirmed the potential of the two regions for sarcophagid species identification.     

Validation of a multiplex PCR assay for the forensic identification of Indian crocodiles

A dependable and efficient wildlife species identification system is essential for swift dispensation of the justice linking wildlife crimes. Development of molecular techniques is befitting the need of the time. The forensic laboratories often receive highly ill-treated samples for identification purposes, and thus, validation of any novel methodology is necessary for forensic usage. We validate a novel multiplex polymerase chain reaction assay, developed at this laboratory for the forensic identification of three Indian crocodiles, Crocodylus palustris, Crocodylus porosus, and Gavialis gangeticus, following the guidelines of Scientific Working Group on DNA Analysis Methods. The multiplex PCR was tested for its specificity, reproducibility, sensitivity, and stability. This study also includes the samples treated with various chemical substances and exposed to various environmental regimes. The result of this validation study promises this technique to be an efficient identification tool for Indian crocodiles and therefore is recommended for forensic purposes.     

Choosing relatives for DNA identification of missing persons

DNA-based analysis is integral to missing person identification cases. When direct references are not available, indirect relative references can be used to identify missing persons by kinship analysis. Generally, more reference relatives render greater accuracy of identification. However, it is costly to type multiple references. Thus, at times, decisions may need to be made on which relatives to type. In this study, pedigrees for 37 common reference scenarios with 13 CODIS STRs were simulated to rank the information content of different combinations of relatives. The results confirm that first-order relatives (parents and fullsibs) are the most preferred relatives to identify missing persons; fullsibs are also informative. Less genetic dependence between references provides a higher on average likelihood ratio. Distant relatives may not be helpful solely by autosomal markers. But lineage-based Y chromosome and mitochondrial DNA markers can increase the likelihood ratio or serve as filters to exclude putative relationships.     

采用Identifiler系统判定消化系统肿瘤组织身源准则探讨

目的 探讨采用Identifiler系统进行消化系统肿瘤组织身源判定时共有等位基因数(IAn)和全相同基因座数(A2)的标准.方法 在对l05对消化系统肿瘤组织-身源正常组织对进行Identifiler系统分型的基础上,依据IAn和A2的有限分布原则,将IAn的16种取值代入已建立的相应判别函数,通过Fisher判别准...