牛传染性鼻气管炎病毒SYBR GreenⅠ实时荧光定量PCR检测方法的建立

为了检测牛传染性鼻气管炎病毒,本研究建立了牛传染性鼻气管炎病毒SYBR Green I实时荧光定量PCR检测方法。通过设计特异性引物扩增病毒基因,将扩增的目的片段(119 bp)连接到pMD19-T载体中,构建重组质粒。将重组质粒作为阳性标准品,用于SYBR Green I实时荧光定量PCR检测方法的建立。结果显示,特异性引物扩增的目的片段约为119 bp,符合预期的大小,经测序鉴定所扩增的目的片段正确。敏感性结果显示,用该方法检测阳性质粒标准品的最低限度为3.04 X101 copies/mL,是Nano-PCR的10倍。将牛病毒性腹泻病毒、牛呼吸道合胞体病毒、牛副流感病毒3型与牛传染性鼻气管炎病毒进行特异性检测。结果显示,只有牛传染性鼻气管炎病毒被检测到,其他病毒均未有特异性的扩增曲线,表明该方法的特异性良好。批内和批间重复变异系数均小于1%,显示该方法具有良好的重复性。用建立的方法对20份疑似IBRV的临床样品进行检测,并与Nano-PCR检测方法进行对比,结果显示本方法的阳性样晶率高于Nano-PCR检测方法。本研究建立的牛传染性鼻气管炎病毒SYBR Green I实时荧光定量PCR方法特异性好、灵敏度高、重复性好,可用于牛传染性鼻气管炎病毒的检测。     

侵权行为法中公平责任的适用范围

侵权行为法中的公平责任是以衡量当事人之间的利益关系为主要理由,来确定责任主体赔偿或补偿责任的一种责任认定方式,是对传统归责方法的一种矫正。公平责任受其特定的归责理由的限制,主要适用于监护人责任、雇主责任、紧急避险责任中某些特定的情况,也适用于受益人的补偿责任或责任分担和对赔偿数额的限制方面。从立法例和司法实践中准确把握公平责任适用范围,对正确认识和运用公平责任归责方法,具有重要司法实践意义。     

Significant Differences in PostMortem Heart Weight Before and After Dissection Using the Short-Axis Dissecting Method

Correctly assessing heart weight can be critical at postmortem examination. The current international guidelines advocate using the short-axis method in dissecting the heart and the heart weighed when the blood is emptied. However, it did not specify at what point the heart should be weighed or how the blood should be emptied. This study compared heart weights at three different time points during the heart examination (immediately after dissecting out of the pericardial sac with blood still in chambers, blood washed/removed from heart chambers without the heart opened, and the heart completely opened, blood emptied, and pad dried). This was to illustrate the variation in measurement and potential errors when the heart is weighed at different time of dissection. The results show that there were statistical and clinical significant differences between the heart weights at each weighing points. We recommend the heart to be completely dissected with any blood and residual washing/rinsing water emptied before being weighed. Although performed in this study, the effect of pad drying the heart on heart weight was not explored and was a limitation in this study.     

The Accuracy and Repeatability of Reconstructing Single Bullet Impacts Using the 2D Ellipse Method

When a bullet punctures a surface, it leaves behind a bullet impact, which can be analyzed in order to determine the origin and trajectory path from which a bullet was discharged using many different scientific methods to reconstruct a shooting scene. The purpose of the research was to test the accuracy and repeatability of reconstructing the impact angle of single bullet impacts using the ellipse method. The research was conducted by firing various calibers of ammunition into drywall panels positioned at varying angles of incidence from 88° until ricochet occurred. This was done in order to determine which caliber type and angle of incidence are most accurate and precise for reconstructing a shooting scene. The study examined four caliber types fired into drywall panels, with 5 repeated shots for each of the 11 angles of incidence (n = 220). Furthermore, 31 participants partook in the study to estimate a bullet impact angle of incidence utilizing the ellipse method. The results show that the best performance (accuracy and repeatability) is seen with the measurements of the 0.45 caliber ammunition. When angle of incidences is low (<64°), the performance (accuracy and repeatability) was seen to be better in all caliber ammunitions. Overall, the data provided for single bullet impacts deposited in drywall show that the ellipse method is useful in providing measurements for most crime scene reconstruction purposes and has also demonstrated that results vary depending on the type of ammunition, firearm, and angle of incidence being examined.     

量刑方法论——从对传统观念的检讨与批判谈起

对犯罪人裁量决定刑罚是刑事审判中重要的环节,事关对司法活动公平与正义的评价.而正确把握量刑的方法,对做到"罚当其罪"和"罪刑相适应"至关重要.从传统观念的检讨与批判谈起,论述量刑的理念、原则、方法和因素,并在量刑的步骤和方法上提出见解.     

The social semiotic of homophone phrase substitution in Chinese netizen discourse

The paper addresses an ingenious way by which Chinese netizens manage to reach their intended audiences under the radar of censors surveying the Internet in China. One specifically Chinese social use of language in this respect is the option to express criticism by using inoffensive characters that share the same or similar pronunciation with politically sensitive phrasing. We identify this type of expression as homophone phrase substitution (HPS). The device has been part of Chinese since ancient times under the name political metaphor. We address the main constraints at work in HPS, especially the role of voice (prosodic contour) that guides the construction of two divergent kinds of aboutness. Leaving aside its critical function in contemporary society, by contrasting HPS with verbal irony, metaphor, and Australian rhyming slang, and by drawing on semiologie and semiotics, indirect speech acts and implicit deixis, we suggest that this linguistic option throws new light on the semantic/pragmatic distinction.     

问题教学法的实践与思考

运用问题教学法需要技巧并坚持一定的原则,只有这样才能取得良好的教学效果,学生才能从中受益。     

刑侦画像造型训练方法的研究

刑侦画像是依据目击者的记忆语言描述,利用素描等手法模拟刻画犯罪嫌疑人相貌特征的技术手段。刑侦画像造型训练方法的研究,为刑侦画像技术的教育实践工作探索科学有效的培养训练方法,以适应我国公安刑侦工作的实际需要。     

Limits of regulatory responsiveness: Democratic credentials of responsive regulation

Responsiveness and accountability constitute the process of democratic representation, reinforcing each other. Responsiveness asks elected representatives to adopt policies ex ante preferred by citizens, while accountability consists of the people's ex post sanctioning of the representatives based on policy outcomes. However, the regulatory literature tends to interpret responsiveness narrowly between a regulator and regulatees: the regulator is responsive to regulatees’ compliance without considering broader public needs and preferences. Democratic regulatory responsiveness requires that the regulator should be responsive to the people, not just regulatees. We address this theoretical gap by pointing out the perils of regulatory capture and advancing John Braithwaite's idea of tripartism as a remedy. We draw out two conditions of democratic regulatory responsiveness from Philip Selznick – comprehensiveness and proactiveness. We then propose overlapping networked responsiveness based on indirect reciprocity among various stakeholders. This mechanism is the key to connecting regulatory responsiveness with accountability.     

Productivity,craftwork and flexibility: the Mobil agreements

ABSTRACT

Using original documents, this paper describes and analyses the case study of one of the productivity bargaining agreements from a leading exponent of productivity bargaining, Mobil’s Coryton Refinery. As well as being important in the UK oil industry these agreements spread throughout the economy. They resulted in major concessions by the unions to relax inter-craft and craft-production trade boundaries and to changes in work methods. These are illustrated in detail and placed in context. Such changes were heralded as ‘new look industrial relations’ and as such were based on a conception of joint regulation. However, other interpretations are possible, and these are then explored and discussed.