Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

宪政维度中的刑讯逼供与罪刑法定

罪刑法定原则是刑法的根本原则之一,而刑讯逼供在我国是一个在司法中久禁不止,且甚为普遍的现象.刑讯逼供罪具有独特的多重当事人结构,与宪政视野的罪刑法定原则相契合.罪刑法定原则的设置源于宪政意义上的限制权力、保障人权的考虑.     

法的价值研究及方法刍议

法的价值是法和法律制度的重要因素和特质,也是法学理论中的难点问题.法律实践和法学理论的许多同题都需要研究法的价值问题,而法的价值的研究方法制约着法的价值研究的深入.只有通过认识主体与客体之阃的关系,坚持主体需要的客观性、阶级性和社会性,采用从主体需要入手的方法,才能正确认识和诠释法的价值问题.     

论侦查权的性质与特征

侦查权在我国被认为是一种司法权,这导致犯罪嫌疑人在认为侦查机关的行为违反法律规定时无权申请法院进行审查,这对于保护被追诉人的权利是极其不利的。从本质上来看,侦查权是一种执行权,这与行政权是一致的,而与司法权有着根本的差异。从基本特征来看,侦查权在价值与功能、体制与人员以及权力运作方面也与司法权有着根本的差异。     

活血通脉颗粒对真性红细胞增多症患者血栓弹力图K值的影响

目的 观察活血通脉颗粒治疗真性红细胞增多症（polycythemia vera,PV）的临床疗效,及其对血栓弹力图K值的影响。方法 随机将60例PV患者分为试验组、对照组各30例。对照组给予西药羟基脲治疗;试验组在对照组的基础上加用活血通脉颗粒。治疗6个月后,观察和比较两组PV患者临床疗效、中医证候评分、外周血象及血栓弹力图K值。结果 两组患者临床疗效分布比较,差异具有统计学意义（P<0.05）;两组患者治疗后证候评分均较治疗前显著降低（P＜0.05）,试验组治疗前后中医证候评分差值显著大于对照组（P＜0.05）;两组治疗后血常规各项指标均较治疗前显著降低（P＜0.05）,试验组治疗前后血小板计数差值显著大于对照组（P＜0.05）;两组患者治疗后血栓弹力图K值均较治疗前显著升高（P＜0.05）,试验组升高至正常值范围,其升高值显著大于对照组（P＜0.05）。结论 活血通脉颗粒可明显改善PV患者中医证候及外周血象,并通过诱导血小板表面受体表达,改善PV患者凝血功能。     

陪审团在死刑案件中适用的价值分析

死刑案件作为最严重的案件,作为关涉公民生命权的案件,它的审判是我们在构建正当法律程序时需要关注的重点。文章以价值分析为主视角,从哲学思辨和实证分析入手,提出在我国的死刑案件中引入陪审团审判,以构建强大的死刑案件普通审程序之设想,试图通过在死刑案件中引入陪审团审判,扩大刑事司法中的民众参与和司法民主,促进司法公正和社会正义之实现。     

警察武力之善法良治——论警察武力法的正义价值标准

警察武力法的正义价值标准由标示正义价值方向的基本理念和标示正义价值尺度的基本原则两项基本要件分级承担并贯彻。中国警察武力法有:社会正义与法律正义有机统一、保权与控权有机统一和价值、规范、事实有机统一三个居位"法上之法"的基本理念和危害行为与武力行为法定、危害行为与武力行为相当、武力行为克减和多样化和正当程序四个居位"法上之法"的基本原则。上述基本理念和基本原则,对系统理解、把握中国警察使用武力的基本原则和构成要件、各种规范的内在关连性,以及构思未来的、"应然"的中国警察武力立法具有不可或缺的重要意义。     

美国诱惑侦查制度及对我国的启示

美国社会执法实践中广泛存在诱惑侦查的使用，关于诱惑侦查的法律和制度规制经过长期演变和经验积累，形成了较为完整的诱惑侦查制度。本文通过对美国诱惑侦查制度的法律规定和具体实践的探究，以期为完善公安机关执法工作提供一些参考。我们应借鉴美国关于诱惑侦查方式运用的有益经验，加快有关诱惑侦查领域的立法进程，明确具体的法律规定。公安机关在执法实践中应正确认识诱惑侦查的合理性，慎用诱惑侦查手段，严格遵循程序执法，规范自身执法行为，积极改进执法方式。     

浅析拐卖人口案件侦查之买方市场的防控

拐卖人口现象屡禁不止,根本原因之一是买方市场的存在及发展.本研究基于对拐卖人口买方市场存在发展的背景条件以及当前形势和防控难点进行分析研究,指出买方市场组成结构的复杂性与单方面打击不协调等问题.针对当前背景下采取系统作战方式应对拐卖人口新特点的重要性,通过咨询专家、文献研究以及与主要案发地区一线民警深入沟通,给买方市场防控措施提出相关建议,以期望对拐卖人口案件侦查有一定借鉴意义.