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41.
Linnet K Johansen SS Buchard A Munkholm J Morling N 《Forensic science international》2008,179(1):78-82
On the basis of simultaneously sampled postmortem blood specimens from the left and right femoral veins the pre-analytical variation of methadone measurements was evaluated and compared to the analytical variation. The material consisted of a series of 27 duplicate samples from routine autopsy cases comprising mainly drug addicts. A chiral LC-MS/MS method was used for measurement of the R- and S-enantiomers of methadone and its main metabolite 2-ethyl-1,5-dimethyl-3,3-diphenylpyrrolinium (EDDP). The analytical CV% was determined to be in the range 3-4% for methadone enantiomers and 4-6% for EDDP enantiomers. The total measurement uncertainty (CV(T)) was estimated from the pre-analytical variation (CV(PA)), analytical variation proper (CV(A)), and variation related to calibration (traceability) (CV(Cal)) according to the relationship CV(T) = [CV(2)(PA) + CV(2)(A) + CV(2)(cal)](0.5). Uncertainty related to calibration concerned a component related to the purity of drug reference compound and a contribution from the production of calibrator solutions (CV(Cal)<1%). Pre-analytical sampling variation was estimated from the duplicate measurements of blood samples after subtraction of the analytical component. The pre-analytical variation amounted to a CV% of 19-21% for R- and S-methadone and 30-38% for R- and S-EDDP, i.e. considerably larger than the other components. Due to the squared addition principle, the resulting total uncertainty (CV(T)) became largely identical to the CV(PA), i.e. 19-21% for R- and S-methadone and 31-38% for R- and S-EDDP enantiomers. Accordingly, CV(T) exceeded CV(A) by a factor 5 or more. Dominance of the pre-analytical component of variation may also be likely for other compounds measured in postmortem blood samples. Thus, the width of the 95%-uncertainty interval (+/-2CV(T)) for a postmortem measurement is largely determined by the pre-analytical component of variation. This should be kept in mind when judging on the uncertainty of postmortem measurement results. 相似文献
42.
Himmelberger AL Spear TF Satkoski JA George DA Garnica WT Malladi VS Smith DG Webb KM Allard MW Kanthaswamy S 《Journal of forensic sciences》2008,53(1):81-89
The 608-bp hypervariable region 1 (HV1) sequences from 36 local dogs were analyzed to characterize the population genetic structure of canid mitochondrial DNA (mtDNA). Sixteen haplotypes were identified. A 417-bp segment of this sequence was compared with GenBank sequences from a geographically representative sample of 201 dogs, two coyotes, and two wolves. Sixty-six haplotypes were identified including 62 found only in domestic dogs. Fourteen of these correspond to the 16 local haplotypes and were among the most frequent haplotypes. The local sample was judged to be representative of the much broader geographic sample. No correlation was observed between local haplotypes and the owner's characterization of dog breed. A 60-bp variation "hotspot" within the canid HV1 was identified as a potentially valuable molecular tool, particularly for assaying limited or degraded DNA samples. 相似文献
43.
尚雷鹏 《湖北警官学院学报》2004,17(5):30-33
现在我们普遍认为 ,理解了人类基因组的遗传变异就可以揭示人类疾病的易感性以及疗效差异的原回。本文将主要讨论几种不同的确认和探测遗传变异的方法。不同的等位基因识别方法在形式上可以分为以下几种 :1、酶学方法 ;2、电泳的方法 ;3、遗传变异的固相识别方法 ;4、层析法 ;5、其他的识别等位基因变异的物理学方法。本文主要介绍那些曾被广泛使用的技术方法 ,同时也涉及到几种虽然没有广泛使用但却具有极大潜在价值的方法 ,是对近几年来在法医物证领域中常用基因分型方法的总结。 相似文献
44.
尚必武 《浙江省政法管理干部学院学报》2013,(5):10-17
20世纪90年代以降,以北美为主导的女性主义叙事学得到了长足的发展,无论是在叙事理论建构还是在叙事批评实践上均取得了引人瞩目的成果.本文主要从性别、叙事性、研究方法以及研究课题等层面出发,评析英国学者露斯·佩奇的女性主义叙事理论.文章指出,一方面,佩奇采用文学与语言学的双重视角,对女性主义叙事学的发展做出了重要贡献;但另一方面,佩奇关于叙事形式可以脱离性别的论点又偏离了女性主义叙事学研究的主流. 相似文献
45.
董明 《浙江省政法管理干部学院学报》2005,(4):62-65
译界人士对翻译理论中的许多问题存有争议.许多争议可以在进化论的指导下得以解决.本文尝试用进化论中的"进化"、"系统"、"变异"等重要概念对翻译理论进行历时和共时的研究,历时为"进化",共时为"系统","变异"赋予翻译理论新的内涵和外延.翻译理论家要带着发展的眼光看待译学的发展. 相似文献
46.
Paul HintonAuthor VitaeEdwin BakerAuthor Vitae Chris HillAuthor Vitae 《Computer Law & Security Report》2012
While latency issues have always existed in IT systems, it is only in recent years that latency has become a significant focal point for both IT suppliers and customers. This paper will provide an overview as to how latency arises in IT systems, why latency is becoming important across a variety of business sectors and then explores some of the prominent legal issues inherent in contracting for low-latency solutions. 相似文献
47.
Chi-Man Song 《社会征候学》2018,28(2):230-256
The purpose of the present analyses of regional slogans is to provide a semiotic perspective for understanding the symbolic communication systems involved in the construction of Korean regional governments. This study attempts to evaluate the slogans in terms of the semantic and morphological aspects of the texts. Findings show an overuse of signs within the slogans of regional governments that weakened the delivery of information regarding local identity. A leading slogan for a large region may create a unique identity in relative terms, whereas second-tier regions tend to mimic the success of others. The present research illustrates that each region of Korea cannot be differentiated in terms of a semantic analysis of linguistic signs. The communication tendencies that influence the creation of slogans for Korean regions are morphologically complex but semantically simple, resulting in the failure of these regions to secure distinctiveness in their individual brand identities. 相似文献
48.
税收为法定之债,于法律规定之税收构成要件充分时而发生,且在发生后原则上即具有不可溯及变更和消灭性。但在法定情形下,作为例外,对已经成立的税收之债可进行溯及调整。税收之债溯及变更和消灭的事由包括基础处理决定的作成、废弃或变更,法律行为的无效、撤销、解除、附条件的成就,形成权的行使,税收优惠条件的丧失,税收法律的溯及适用,强制执行的回转等。溯及调整税收债务,应遵循一般程序和特别程序的规定。 相似文献
49.
Yongshin Kim 《The Pacific Review》2019,32(3):291-312
By comparing three Chinese pillar industries, automobile, petroleum, and machinery, this paper analyzes how the development of uneven institutional configurations bred sectoral variation in transitional China. In 1994, at the dawn of the Chinese socialist market economy, the State Council issued guidelines for national industrial policy and proposed the enactment of similar growth-promoting policies for all pillar industries. The level of policy enactment and the resulting outcomes, however, vary significantly across the industries. Previous literature on China's rapid industrialization has sought to build a single model encompassing China as a whole, and has emphasized either the state's institutional capacity or China's unique governance mechanisms. This study investigates China's industrialization by taking account of the uneven development of institutional capacity amid macro institutional reforms. Every idea and institution evolved at a different pace in these reforms, and the incremental nature of the reforms further deepened the gaps between sectors. This paper argues that, due to this uneven development, the Chinese state was not strong enough to be considered a developmental state until at least the early 2000s. 相似文献
50.
While packaging is a common marketing subject, this illustration paper provides an alternative perspective through the linguistic examination of packaging as discourse, focusing on male grooming, masculinity and metrosexuality. Male grooming is often perceived as trespassing into feminine space and creating ambiguity in gender borders. This study aims to explore how packaging designers negotiate masculinity, in order to persuade men to accept grooming products through social interactions within the packaging of L'Oréal Men Expert. Employing Scollon's mediated discourse analysis and incorporating the Barthesian order of signification, the study analyses how different social actions within the packaging discourse are mediated by multimodal features. The findings reveal five negotiating strategies, i.e. constructing a hegemonic masculine image; negotiating (a masculinised) metrosexuality; empowering men; giving men a logical reason to groom; and a different portrayal of skincare products. The key aim of this paper is to illustrate the differences between marketing and linguistics and propose possible collaboration. 相似文献