传染性支气管炎病毒TY1株结构蛋白基因部分片段的表达

根据已报道的传染性支气管炎病毒S基因序列设计了1对引物,利用从传染性支气管炎病毒TY1株中提取的RNA,经PCR扩增获得了300 bp的产物;序列测定与分析表明,所扩增产物是传染性支气管炎病毒结构蛋白基因的部分片段。将扩增片段插入pET32a构建表达载体,并于大肠埃希氏菌BL21中进行表达;结果表明,该片段在大肠埃希氏菌中成功表达,产物为30 ku、以可溶性形式存在的蛋白。Western-blotting分析表明,该蛋白可与传染性支气管炎病毒TY1株阳性血清发生反应。     

浅析模糊限制语在政治访谈中的表现形式

模糊限制语(hedge)是指一些“把事物弄得模模糊糊的词语”。这些词语就话语的真实程度或涉及范围,对话语的内容进行修正或限定,表明说话人的态度、主观认识与评价。英语中存在着大量模糊限制语。模糊限制语不仅是说话者保持面子的一种策略,而且在政治访谈中更是一种目标。     

试析公安机关起诉意见书中运用的表达方式

起诉意见书是公安机关办理刑事案件活动中一种重要的法律文书,在整个刑事诉讼活动中占有重要的地位,具有重要的意义,所以必须把该文书制作得规范合法。而要使该文书制作得规范合法,一方面应了解该文书的格式、结构,另一方面则应准确把握该文书中使用的表达方式。起诉意见书中使用的表达方式有说明、叙述和议论。     

遮蔽与敞亮的心灵画幅——伊蕾诗歌现象批评

伊蕾是当代文学史上诗歌新生代时期的一位独特的女诗人,她的作品是一个时代的心灵缩影,其诗歌意识及艺术表现形式都值得人们予以应有的关注.     

复发性流产肾虚证小鼠子宫蜕膜组织miRNA表达谱检测及生物信息学分析

目的 探讨微小RNA（microRNA,miRNA）在复发性流产（repeated spontaneous abortion,RSA）肾虚证小鼠子宫蜕膜组织中的表达特征。方法 运用基因芯片技术对所采集的正常对照组和RSA小鼠蜕膜组织进行miRNA检测,运用Genespring软件对miRNA进行筛选及功能分析。应用生物信息学方法分析差异表达的miRNA及其靶向基因在RSA发生、发展中的作用。结果 芯片检测结果显示正常对照组和RSA肾虚证组筛选出差异表达的miRNA共有63条（22条上调、41条下调）,实时荧光定量PCR验证的miRNA的表达情况与miRNA芯片结果一致。京都基因与基因组百科全书通路富集分析及基因本体分析显示,其差异表达的miRNA涉及到PI3K-Akt信号通路、细胞吞噬通路、癌症通路、黏着斑通路、肌动蛋白细胞骨架调控通路、轴突导向信号、MAPK信号通路、PAP-1信号通路等。结论 RSA肾虚证小鼠子宫蜕膜组织中存在差异表达的micro RNA,可能涉及RSA肾虚证的发生、发展。     

苗族史诗《亚鲁王》洪水神话的水文化表达

苗族史诗《亚鲁王》记载的洪水神话是迄今发现较为完整的苗族洪水神话版本,在水文化表达上突显了苗族西部方言区的文化特色。《亚鲁王》的洪水神话通过祖先谱系、音乐起源和英雄时代等文化方式表达了民族文化认同、民族文化特色和地域民间信仰。洪水再生神话是对数百代祖先造人努力的一个总结,洪水神话起因中带有音乐起源和女性悲剧,洪水神话之后人类找到新的家园,标志着从神的时代走向英雄时代,从而开启了亚鲁王信仰的英雄时代。史诗的洪水神话表达反映了中华多元民族文化的凝聚力,推动中华文化自信和中华民族文化共同体建设。     

Zombies,Gender, and Student Active Learning

Abstract

While the use of active learning exercises and the incorporation of popular culture in the classroom have increased in recent years, the study of potential gendered effects on learning and engagement when it comes to these practices has been limited. In this study, data are collected from international politics courses using a zombie outbreak as the theme for a United Nations simulation to explore whether there are unintentional gendered outcomes from these activities. Contrary to expectations of previous literature, no statistical differences manifest between men and women in enjoyment and learning when using zombie themes in active learning.     

Working Girls: Femininity and Entrapment in Peyton Place and Valley of the Dolls

Jacqueline Susann's Valley of the Dolls (1966) and Grace Metalious's Peyton Place (1956) are novels that have long exerted a powerful hold on the popular imagination. The bestselling Peyton Place was adapted into a successful film in 1957 before becoming an iconic television series, running from 1964 to 1969. Valley of the Dolls was similarly re-imagined in two films, Valley of the Dolls (1967) and Beyond the Valley of the Dolls (1970), which, even today, retain a cult following. These books are typically remembered for their scandalous bringing to light of such ‘taboo’ issues as adultery, abortion, female sexuality and sexual abuse. But this article suggests that Peyton Place and Valley of the Dolls are equally preoccupied with a sympathetic examination of the role of women in the post-war workplace. In both of these novels, the process of female self-fashioning is integrally related to a woman's entry into the workforce, and to the making and controlling of her own money. But this entry into the male-dominated workforce is inherently fraught with danger, and Metalious and Susann expose some of the myriad ways in which the so-called ‘American Dream’ is contingent on the entrapment, suppression and regulation of various forms of female desire and agency.     

The legacy of subalternity and Gramsci’s national–popular: populist discourse in the case of the Islamic Republic of Iran

Drawing on Laclau’s concept of populist discourse and Gramsci’s ‘national–popular collective will’, and using the case of Iran, this article puts forward the idea of the legacy of subalternity in the context of post-revolution governments. The concept of ‘national–popular collective will’ facilitates an understanding of how the popular subject is constructed and the meanings embedded in that process. It is argued that Islamic Republic elites articulate a populist discourse that constructs the ‘self’ (the Islamic Republic) as synonymous with ‘the people’. Embedded in this discursive construction is a legacy of subalternity that goes back to the 1979 Revolution’s populist discourse.     

Five years after the Arab Spring: a critical evaluation

A new political geography has emerged in the Middle East and North Africa (MENA) after the Arab Spring. The transformative impact of the popular upheavals appeared to put an end to long-term authoritarian regimes. Today, the region is far from stable since authoritarian resilience violently pushed back popular demands for good governance and is pushing to restore former state structures. However, the collective consciousness of the popular revolts endures, and a transformative prospect may emerge on the horizon. The chaotic situation is the result of an ongoing struggle between those who seek change and transformation and others in favour of the status quo ante. A critical evaluation of the Arab Spring after five years indicates a continuous process of recalculation and recalibration of policies and strategies. There are alternative routes for an eventual settlement in the MENA region, which are in competition against both regional and transregional quests for a favourable order.