肺气虚证模型豚鼠肺功能与血气分析的变化

目的:观测肺气虚证模型豚鼠肺功能与血气分析的变化。方法:采用卵白蛋白(OVA)复制哮喘模型并加以改良法,建立肺气虚证模型豚鼠;对正常对照组(对照组)、肺气虚证模型组(模型组)两组动物进行肺功能与血气分析的检测。结果:与对照组比较,模型组肺功能指标吸气阻力(Ri)与呼气阻力(Re)均显著增加,第0.3秒用力呼气容积(FEV0.3)及肺顺应性(Cldyn)下降,血气分析中氧分压(pO2)、氧饱和度(SaO2)及酸碱值(pH)降低,而二氧化碳分压(pCO2)则升高。结论:肺气虚证有一定的肺功能损害,并呈现低氧与高碳酸血症。     

婴幼儿猝死综合征心传导系统的免疫组化研究

作者应用ＰＧＰ９．５与抗Ｓ１００抗体和高压消毒蒸锅抗原复活技术对４例婴幼儿猝死综合征（ＳＩＤＳ）和３例非心源性死亡对照婴儿心传导系统的神经组织总量进行免疫组化研究。结果：在正常婴儿心传导系统，ＰＧＰ９．５与Ｓ１００阳性的神经纤维里不均匀分布，以窦房结最多，房室结次之，希氏束最少；４例ＳＩＤＳ心传导系统的神经组织分布同对照组无明显差异。     

Floppy aortic valve presenting as sudden death

A previously healthy 34-year-old Dutch immigrant to Australia died unexpectedly in his sleep. At autopsy the only significant finding was a floppy aortic valve (FAV). Histologic, histochemical and electron microscopic studies corroborated the macroscopic diagnosis. Previously described associations of the FAV include the floppy mitral valve, Marfan's syndrome, aortic root dilatation and aortic cystic medial necrosis. None of these features were found in the present case which is the first recorded example of isolated FAV presenting as sudden death. The mechanism of death is obscure, and while it is presumed to be dysrhythmic, a detailed histological examination of the cardiac conducting system revealed no anatomic abnormality.     

婴儿急死综合征(SIDS)颈动脉体的超微结构

作者利用透射电镜对8例婴儿急死综合征死亡及4例其他原因死亡的婴儿的颈动脉体进行观察。发现婴儿急死综合征与非婴儿急死综合征死亡的婴儿,其颈动脉体的主细胞形态结构相似。两组检材的主细胞内均含大量致密颗粒,其大小及数量无明显不同。认为有必要进一步应用生化技术对婴儿急死综合征颈动脉体主细胞中致密颗粒物质进行定性和定量研究。     

痰瘀同治必调气

“痰瘀同病”为许多慢性病、老年病之主要病机，“痰瘀同治”为其基本治则，然而，气的失常既为痰，瘀之病理基础，又为痰瘀同病之继发病变，互为因果，痰瘀同治之中，又须参入行气，补气之品。     

养阴抗毒胶囊对糖皮质激素致阴虚大鼠血浆ACTH、CORT的影响

目的:探讨养阴抗毒胶囊2号(YC2)防治糖皮质激素(GC)不良反应的机制。方法:SD大鼠60只,随机分为空白对照组,阴虚模型组,YC2(高)、(中)、(低)组,六味地黄丸组。在中药预处理及模型复制给药9 d后,取血,用放射免疫方法检测各组血浆促肾上腺皮质激素(ACTH)及皮质醇(CORT)的含量。结果:阴虚模型组ACTH及CORT含量明显高于空白对照组,各中药组血浆ACTH及CORT含量均不同程度低于阴虚模型组。结论:GC所致“阴虚”证与下丘脑-垂体-肾上腺轴(HPA)功能亢进密切相关,YC2明显改善GC作用下HPA轴功能亢进的状态,可能是其预防和治疗GC不良反应的机制之一。     

猪生殖与呼吸综合征病毒HN6株ORF5基因的克隆及其在大肠杆菌中的表达

用RT-PCR方法从河南分离的1株(HN6)猪生殖与呼吸综合征病毒(PRRSV)核酸中扩增缺失N端疏水序列的基因片段dORF5(deleting ORF5),并将其克隆到pMD18-T载体中测序,再亚克隆到原核表达载体pET-32a上。重组质粒转化大肠杆菌BL21,用不同浓度IPTG分别于37℃诱导,经SDS-PAGE分析,所表达的融合蛋白的分子质量约31.4 ku,薄层扫描分析显示,表达量占菌体总蛋白含量的28.8%。Western-blotting结果表明,重组蛋白可被PRRSV阳性血清所识别。证实,该蛋白可用于PRRSV的诊断。     

KCNE、KCNQ1与心性猝死的相关性研究进展

部分心性猝死由于缺乏明确的病理学改变,其鉴定工作一直是法医工作者的一大难题。近年来,与长QT综合征、心房颤动等致死性心律失常疾病相关基因（KCNE基因家族与KCNQl）等研究逐渐增多。国内外研究发现KCNE和KCNQ1基因编码心肌钾离子通道,其基因异常可引起严重的心律失常,甚至导致心性猝死。因此,死后KCNE和KCNQl的基因检测对于心性猝死鉴定具有重要意义。本文对KCNE、KCNQl与心性猝死的相关性研究进展进行综述,希望能为法医学研究和实践提供参考。     

Exploring gender differences in constellations of problem behaviors and associated health-related factors during adolescence

Using data from the National Longitudinal Study of Adolescent Health (Add Health), this study expands on previous research on adolescent problem behavior by (1) examining gender differences in patterns or ‘subgroups’ of adolescents based on self-reported problem behaviors and (2) identifying differences in health-related factors including service utilization, physical and mental health, and violent victimization across the identified gender-specific subgroups. The data used in this study were taken from Wave 2 of the National Longitudinal Study of Adolescent Health (Add Health) data and includes respondents under the age of 18 (n?=?10,360). Based on 16 problem behavior items measuring delinquency, substance use, risky sexual practices, and status offending, latent class analyses identified a 4-class model for the male subsample and a 3-class model for the female subsample. Important differences in health-related factors were observed across the latent classes. However, these differences were fairly consistent for boys and girls. Implications for prevention and intervention strategies, specifically focusing on the intersection of juvenile justice and public health services, are discussed.     

A Strategic Prospect that Can Be Realized

正The Chinese Dream in Full Swing Speech Extracts from the International Dialogue on the Chinese Dream ForumTHE Chinese Dream encompasses four main visions:a renewed,powerful China;a civilized China governed by equality and fairness;a harmonious China in which all social strata coexist peacefully;and a beautiful China with a clean environment.Despite China’s well-known difficulties–pollution,corruption,and economic inequality–China