猪生殖与呼吸综合征病毒HN6株ORF5基因的克隆及其在大肠杆菌中的表达

用RT-PCR方法从河南分离的1株(HN6)猪生殖与呼吸综合征病毒(PRRSV)核酸中扩增缺失N端疏水序列的基因片段dORF5(deleting ORF5),并将其克隆到pMD18-T载体中测序,再亚克隆到原核表达载体pET-32a上。重组质粒转化大肠杆菌BL21,用不同浓度IPTG分别于37℃诱导,经SDS-PAGE分析,所表达的融合蛋白的分子质量约31.4 ku,薄层扫描分析显示,表达量占菌体总蛋白含量的28.8%。Western-blotting结果表明,重组蛋白可被PRRSV阳性血清所识别。证实,该蛋白可用于PRRSV的诊断。     

Examining the relationships between sub-clinical psychopathic traits with shame,guilt and externalisation response tendencies to everyday transgressions

Although many theories of psychopathy include reference to some form of emotional deficit, surprisingly little research has examined the relationships between psychopathic traits and important self-conscious moral emotions such as shame and guilt. The present study sought to examine these relationships in a sub-clinical sample, taking into account the important theoretical differences between the two emotions. Participants (N = 739) completed a measure of psychopathic traits and a measure of self-conscious affect style. Both primary and secondary psychopathic traits were found to be inversely related to guilt-proneness; however, the effect size was greater for primary psychopathic traits. Primary psychopathic traits were unrelated to shame-proneness, while secondary psychopathic traits were positively related to shame-proneness. Both primary and secondary traits were positively related to externalisation; however the effect size was greater for primary over secondary traits. The findings provide support for affective differences between psychopathy variants.     

Reference Dependence in Political Primaries

This research considers how reference dependence impacts choice in a primary election. The normative advice is to weigh personal political preference against the greater ability of a more electable candidate to win the later general election. Here a behavioral view of primary elections is developed by adding reference dependence to a Hotelling model of political competition. The model details the impact of references on voter choice and generates recommendations as to the reference marketers for any candidate would like primary voters to employ. The advice to a more electable, that is, moderate, candidate is to encourage voters to compare the primary candidates to the extremes of the opposite party. A less electable candidate should encourage voters to compare the candidates to positions within their own party.     

KCNE、KCNQ1与心性猝死的相关性研究进展

部分心性猝死由于缺乏明确的病理学改变,其鉴定工作一直是法医工作者的一大难题。近年来,与长QT综合征、心房颤动等致死性心律失常疾病相关基因（KCNE基因家族与KCNQl）等研究逐渐增多。国内外研究发现KCNE和KCNQ1基因编码心肌钾离子通道,其基因异常可引起严重的心律失常,甚至导致心性猝死。因此,死后KCNE和KCNQl的基因检测对于心性猝死鉴定具有重要意义。本文对KCNE、KCNQl与心性猝死的相关性研究进展进行综述,希望能为法医学研究和实践提供参考。     

Exploring gender differences in constellations of problem behaviors and associated health-related factors during adolescence

Using data from the National Longitudinal Study of Adolescent Health (Add Health), this study expands on previous research on adolescent problem behavior by (1) examining gender differences in patterns or ‘subgroups’ of adolescents based on self-reported problem behaviors and (2) identifying differences in health-related factors including service utilization, physical and mental health, and violent victimization across the identified gender-specific subgroups. The data used in this study were taken from Wave 2 of the National Longitudinal Study of Adolescent Health (Add Health) data and includes respondents under the age of 18 (n?=?10,360). Based on 16 problem behavior items measuring delinquency, substance use, risky sexual practices, and status offending, latent class analyses identified a 4-class model for the male subsample and a 3-class model for the female subsample. Important differences in health-related factors were observed across the latent classes. However, these differences were fairly consistent for boys and girls. Implications for prevention and intervention strategies, specifically focusing on the intersection of juvenile justice and public health services, are discussed.     

A Review of the Forensic Implications of Pica

Pica is characterized by the persistent eating of non‐nutritive substances over some time that is inappropriate for the maturation stage of the individual and is not culturally sanctioned. A 9‐year‐old boy with Goldenhar syndrome, significant developmental delay and pica, collapsed and died after a short history of diarrhea and vomiting. Death was due to a sigmoid volvulus resulting from filling of the distal colon with feces containing dirt, stones, and rice with evidence of ischemic intestinal necrosis. Lethal complications of pica include intestinal obstruction and perforation with peritonitis and generalized sepsis. Other findings at autopsy may include airway obstruction, heavy metal poisoning, and parasitic infestation. Presenting symptoms and signs of such complications may be subtle or masked given the nature of underlying conditions, and so careful evaluation of the medical histories of individuals with pica may be necessary to provide pertinent details of associated medical and psychiatric conditions.     

News Norms and the Strategic Timing and Content of Candidate Messages

Abstract

In this paper, we ask what sets of factors influence the timing and the content of presidential candidate press releases. Utilizing a unique data set, we examine the influence of internal campaign factors as well as external media factors on campaign information dissemination during the invisible primary season. We find that both types of factors may influence the timing of press releases; as to content, however, media norms are less likely to alter a candidate's general strategic message, which is dictated by his competitive status and national stature.     

Exports as a Determinant of Long-Run Growth in Paraguay, 1966-96

During the 1970s and early 1980s Paraguay experienced relatively high rates of economic growth as well as a boom in primary goods production destined for export. The question which this research addresses concerns the relationship between these events and the applicability of the so-called export-led growth (ELG) hypothesis. The hypothesis is investigated via the use of modern time series methods including Granger causality tests, error correction modeling, and vector autoregression. The basic conclusion reached is that the ELG does not have much relevance to the Paraguayan case.     

The Campaign Disconnect: Media Coverage of the 2000 South Carolina Presidential Primary

Campaigns are an interactive process in which candidates, outside groups, the media, and voters communicate with each other to create an information environment that allows the various participants to construct meaning and form an understanding of the candidates and the campaign. Presidential primaries add a layer of complexity to this process as candidates and the press deal with both local and national audiences. In this article, we analyze the campaign communications in the 2000 Republican presidential primary in South Carolina—including candidate ads, mailings, and phone calls; local and national newspaper coverage; and network television coverage. We find that there was a disconnect as news media often focused on events and issues that diverged from the messages of the candidates' campaigns. In addition, we find substantial differences between local and national media coverage of the primary resulting from their distinct audiences and the reporters' own understanding of the local context that created significantly different information environments for voters in the state and those out of the state. We consider the implications of these findings for how voters and journalists understand the candidates as well as the challenges presidential candidates face in simultaneously campaigning locally and nationally.     

青壮年猝死综合征SCN2B、SCN4B基因检测

目的寻找SCN2B、SCN4B基因的变异位点,探讨其与青壮年不明原因夜间睡眠中猝死(SMDS)的关系。方法提取SMDS病例组及健康对照组的基因组DNA,采用聚合酶链式反应(PCR)方法扩增SCN2B、SCN4B基因编码区外显子、外显子-内含子交界区以及3'侧翼区序列,直接行DNA测序以明确遗传变异类型。结果在病例组中共检测到4个变异位点,c.237+27AG,c.*38CT,c.174CT(p.C58C)和c.*7CT。结论本研究首次对中国人SMDS病例进行了SCN2B、SCN4B基因的检测,上述基因是否为中国人SMDS的易感基因尚有待进一步研究证实。