天花粉蛋白致豚鼠全身过敏反应的实验研究

目的：观察天花粉蛋白（ＴＣＳ）注射液致豚鼠全身过敏反应的情况。方法：用ＴＣＳ作为抗原，对豚鼠进行致敏和攻击。结果：ＴＣＳ可引起豚鼠发生全身过敏反应。在一定范围内，剂量与阳性率无明显关系；低于一定剂量，反应减少或不出现；剂量过高亦使反应率降低。随着攻击（致敏）次数和时间增加，阳性反应也相应提高。结论：ＴＣＳ对豚鼠有强的致敏性，剂量过低或过高均对过敏反应发生率有一定的影响。     

DNA Analysis of Natural Fiber Rope*

Abstract: When rope is found at a crime scene, the type of fiber is currently identified through its microscopic characteristics. However, these characteristics may not always unambiguously distinguish some types of rope from others. If rope samples contain cells from the plants of origin, then DNA analysis may prove to be a better way to identify the type of rope obtained from a crime scene. The objective of this project was to develop techniques of DNA analysis that can be used to differentiate between ropes made from Cannabis sativa L. (hemp), Agave sisalana Perrine (sisal), Musa textilis Née (abaca, “Manila hemp”), Linum usitatissimum L. (flax), and Corchorus olitorus L. (jute). The procedures included extracting the DNA from the rope, performing polymerase chain reaction (PCR) using the extracted DNA as a template, and analyzing the DNA products. A primer pair for PCR, chosen from within a chloroplast gene for the large subunit of ribulose bisphosphate carboxylase/oxygenase, was designed to be specific for plant DNA and complementary to the genes from all five plants. The resulting PCR fragments were approximately 771 base pairs long. The PCR fragments, distinguished through base sequence analysis or restriction enzyme analysis, could be used to identify the five different rope types. The procedure provides a useful addition to visual methods of comparing rope samples.     

上海“口岸”流窜犯罪的现状及对策

随着上海经济的高速发展 ,交通口岸的治安状况发生了很大的变化 ,机场治安日益复杂 ,码头治安渐趋平稳 ;长途汽车站治安问题突出 ;火车站社区化 ,流窜犯罪辐射面扩大 ;公路道口打流截逃任务加剧。为此 ,必须建立一套适应新形势发展需要的完善的“口岸”打流机制。     

基于社会互动理论的大学生幸福感研究

基于社会互动理论对大学生幸福感的研究表明,幸福感和心理健康各因子存在年级差异,且独生子女的适应性显著低于非独生子女,心理不平衡性显著高于非独生子女。大学四年级学生和独生子女在完成学生与社会人员的角色演变上存在问题。为此,要教育学生理解家庭与社会的角色期待并提前做好准备,学校教学也应满足社会角色对学生的要求。     

Smart Security For a SmartAge

正security concerns related to iphones prompt Chinese smartphone users to go domesticThe release of the iPhone 6 on September 10 triggered a new round of Apple-maniafor smart phone users around the worldHowever,while many are thrilled with the larger     

The use of Hemastix® severely reduces DNA recovery using the BioRobot® EZ1

Abstract: The choice of reagents for presumptive tests for blood, and subsequent extraction methodologies, can significantly affect both the quantity and quality of purified DNA. Blood samples directly tested with Hemastix^® yielded <1% of the DNA recovered from untested samples when purified using the Qiagen BioRobot^® EZ1 and EZ1^® DNA Investigator kit. Full short tandem repeat profiles were obtained from both tested and untested samples, suggesting that the Hemastix^® reagent(s) affect DNA binding, rather than produce DNA damage. The Hemastix^® inhibition of DNA yield could be overcome by the addition of MTL buffer to the sample prior to extraction. Laboratories may wish to modify current procedures for extracting blood samples, utilize other extraction/purification methodologies, or inform their submitting agencies to avoid direct exposure of questioned bloodstains to Hemastix^® reagents.     

Determination of an effective housekeeping gene for the quantification of mRNA for forensic applications

The potential application of mRNA for the identification of biological fluids using molecular techniques has been a recent development in forensic serology. Constitutively expressed housekeeping genes can assess the amount of mRNA recovered from a sample, establish its suitability for downstream applications, and provide a reference point to corroborate the identity of the fluid. qPCR was utilized to compare the expression levels of housekeeping genes from forensic-like body fluid stains to establish the most appropriate assessment of human mRNA quantity prior to profiling. Although variability was observed between fluids and individuals, results indicated that beta-2 microglobulin exhibited the highest expression for all body fluids examined and across donors. A one-way analysis of variance was performed for housekeeping gene variability between donors (at the α, 0.05, significance level), and the results indicated significant differences for semen, vaginal secretions, and menstrual blood.     

对我国先予执行制度立法定位的思考

先予执行的功能是对民事诉讼起保障作用,其保障具体包含了对当事人合法权益的"及时"保护和"有效"保护两方面的内容。但现行先予执行的立法仅体现了对当事人的"及时"保护,缺失对当事人"有效"保护的规定。先予执行立法定位的偏颇和制度设计的不合理,不仅使当事人合法权益得不到充分的保障,也使先予执行制度在我国目前的司法实务中很少得到施行,欲解决先予执行的现存问题,仅进行制度性的修补无济于事,必须基于对当事人合法权益的"有效"保护和对当事人合法权益的"及时"保护,对先予执行制度进行重新立法定位,将先予执行界定为是使债权人的权利得到早日实现、是对未生效的判决赋予执行力。     

p33.4位点扩增片段长度多态性及其法医学应用的研究

以聚合酶链反应（PCR）、聚丙烯酰胺凝胶垂直电泳和银染法对中国100名无关个体小卫星区域p33．4位点的扩增片段长度多态性（Amp－FLPs）进行了研究，检出了8个等位基因。通过BIOTRAC系统进行数据处理．各等位基因重复单位的数目分别为7、10到15，其中在13～14之间发现一差值不足一个重复单位长度的罕见等位基因。片段长度分布于603～1115bP之间，基因频率分布于0.5～33．5％间，杂合度为64％，DP值为84．5％。对5个家庭25名相关个体进行分析，符合孟德尔遗传定律；对同一个体不同组织的DNA进行P33.4位点的分型研究表明，该技术适宜于法医物证检验。此外，本研究以Chelex处理不同检材制备DNA模板用于扩增，率先建立了比常规方法更快、更简便、更为实用的检验方法。