Fluorescence Imaging of Posterior Spiracles from Second and Third Instars of Forensically Important Chrysomya rufifacies (Diptera: Calliphoridae),

Entomological protocols for aging blowfly (Diptera: Calliphoridae) larvae to estimate the time of colonization (TOC) are commonly used to assist in death investigations. While the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. This pilot study sought to improve resolution of larval stage in the forensically important blowfly Chrysomya rufifacies using high‐content fluorescence microscopy and biochemical measures of developmental marker proteins. We established fixation and mounting protocols, defined a set of measurable morphometric criteria and captured developmental transitions of 2nd instar to 3rd instar using both fluorescence microscopy and anti‐ecdysone receptor Western blot analysis. The data show that these instars can be distinguished on the basis of robust, nonbleaching, autofluorescence of larval posterior spiracles. High‐content imaging techniques using confocal microscopy, combined with morphometric and biochemical techniques, may therefore aid forensic entomologists in estimating TOC.     

海峡两岸深入开展旅游合作的政治经济意义及建议

海峡两岸同文同脉同根,海峡两岸本是一家人。海峡两岸深入开展旅游合作不仅有利于促进两岸人民的交流与合作,增进两岸人民彼此的互信与了解,旱日实现祖国和平统一,还是促进台湾经济发展和惠及台湾人民的战略举措。因此,海峡两岸深入开展旅游合作是在海峡两岸架起友谊的桥梁,有利于发展两岸关系,推进祖国统一大业的早日完成。     

略论“两少一宽”刑事政策——以乌鲁木齐“7·5”事件为视角

乌鲁木齐＂7.5＂事件的发生表明＂两少一宽＂政策的适用存在重大误区：人为地扩大＂两少一宽＂政策的适用空间和犯罪行为的受害对象,从而产生严重的社会后果。全面理解＂两少一宽＂政策的关键要点就在于必须把＂两少一宽＂政策的适用严格限制在民族自治地方的少数民族本民族之间的犯罪行为上,只有这样才能消除＂两少一宽＂政策的适用的＂盗户效应＂。     

Level Two Negotiations: Helping the Other Side Meet Its “Behind‐the‐Table” Challenges

A long analytic tradition has explored the challenge of productively synchronizing “internal” with “external” negotiations, with a special focus on how each side can best manage internal opposition to agreements negotiated “at the table.” Implicit in much of this work has been the view that each side's leadership is best positioned to manage its own internal conflicts, often by pressing for deal terms that will overcome internal objections and by effectively “selling” the agreement to key constituencies. Far less frequently have analysts considered how each side can help the other side with its “behind‐the‐table” barriers to successful agreement. Following Robert Putnam's two‐level games schema, I characterize such “behind‐the‐table” or “Level Two” barriers more broadly, offer several innovative examples of how each side can help the other overcome them, and develop more general advice on doing so most effectively. As a fuller illustration of a Level Two negotiator helping the other side with its formidable behind‐the‐table challenges, I pay special attention to the end‐of‐Cold‐War negotiations over German reunification in which former American Secretary of State James Baker played a key role.     

未来二十年辽宁流动人口犯罪的发展变化预测

未来二十年,辽宁流动人口犯罪将继续保持增长势头,上升比率会随政府相关政策调整有所下降;侵财类案件仍在各类案件中处于高发势头,伤害类案件会有所上升;团伙犯罪及重大、恶性案件的构成比例将有所增加。面对这种发展趋势,形势不容乐观。     

Inherent Fluorescence Detection of Latent Fingermarks by Homemade Shortwave Ultraviolet Laser

Detection of latent fingermarks on various substrates is critical in crime investigations. Conventional chemical methods using reagents could contaminate or even destruct biological information of samples. Here, an optical method and successful case application of detecting latent fingermarks through long‐wave ultraviolet (UV) fluorescence (300–400 nm) by shortwave UV laser excitation is reported. Experimental results indicate that the recovery rate of the latent fingermarks on various paper items is in the range of 70–80% without chemical treatments. Moreover, the optical method allows for the preservation of samples for further examination, such as polymerase chain reaction (PCR) testing. The technique has also been successfully applied to a criminal case in identifying the suspect, which, to the best of our knowledge, has never been reported in real crime investigations. Therefore, such a method as UV‐excited UV fluorescence in detecting latent fingermarks may be better for examination in cases where biological information of samples is needed for consequent testing.     

A City‐wide Investigation of the Isotopic Distribution and Source of Tap Waters for Forensic Human Geolocation Ground‐truthing

Human geolocation is prefaced on the accuracy of the geographic precision of mapped isotopic values for drinking water. As most people live in cities, it becomes important to understand city water supplies and how the isotopic values uniquely reflect that city. This study investigated the isotopic distribution of δ²H and δ¹⁸O from sourced tap waters that were collected from across the Metro Vancouver (MV) area (n = 135). The results revealed that the isotopic values reflect their water sources with a range of 5.3‰ for δ¹⁸O_tap and 29.3‰ for δ²H_tap for MV. The results indicate that individual cities need higher resolution studies to determine their tap water isotopic ranges, and a good understanding of the water supply network itself for human geolocation work. With an extended high‐resolution understanding of each city, human tissue may be compared with more certainty for geolocation.     

关于建立一警双机三管控的调查与思考

推行一警双机三管控机制,主要是通过以社区(责任区)民警为轴心,以社区(责任区)为单位投入户协员、保安员、巡防队员管控辅警力量,可以使松散的群防群治队伍变成具有内在紧密联系的整体,从而构成完整的社区(责任区)治安防范网络。     

高职高专公共事业类专业两年制课程体系改革研究的基本思路

高职高专公共事业类专业两年制课程体系的改革,需要把学科体系变为能力体系,把专业学科为本位变为以职业岗位和工作能力为本位,也就是说,要以就业市场为导向设置课程,其特色具体体现在：各专业根据自身特点,主要根据就业岗位群的职业工作任务核心能力标准,在此基础上分析知识构成,遵循高等教育学习规律设置专业基础课、专业课程和专业延展课程,构建专业核心能力和延展能力培养课程集群,形成将“模块式”与“三段式”综合集成的能力本位为主的新型高职教育课程体系。     

Application of three spectrometric methods to total selenium determination in postmortem material in a case of acute selenium compound poisoning

Three spectrometric methods, that is, spectrofluorimetry (SF), atomic absorption spectrometry with electrothermal atomization (ET-AAS), and atomic fluorescence spectrometry with hydride generation (HG-AFS) were used for the determination of total selenium in biological samples taken from postmortem material in a case of acute selenium compound poisoning. The precision of the SF, ET-AAS, and HG-AFS methods (RSD, n=10) was found to be in the ranges of 10.0-15.0, 3.0-6.0 and 1.0-1.5%, respectively, and the detection limit was 10.0, 4.0, and 0.1 μg/L of Se, respectively. In the case of HG-AFS, the analytical procedure takes less time and is less laborious than the other methods considered. The obtained results show the usefulness of the HG-AFS method as a supplementary analytical tool to the SF and ET-AAS methods with respect to the determination of selenium as well as the possibility of using this method as a primary one in forensic toxicology practice.