Thin layer chromatography/fluorescence detection of 3,4-methylenedioxy-methamphetamine and related compounds

A rapid and sensitive method for the detection of six methylenedioxylatedphenethylamines, 3,4-methylenedioxymethamphetamine (MDMA); 3,4-methylenedioxyamphetamine; 3,4-methylenedioxyethylamphetamine; N-methyl-1-(3,4-methylenedioxyphenyl)-2-butamine; N-methyl-1-(3,4-methylenedioxyphenyl)-3-butamine; and 3,4-methylenedioxydimethylamphetamine, by thin-layer chromatography with fluorescence detection is proposed. These compounds form fluorophores on the developing plate following spraying with a reagent consisting of sodium hypochlorite, potassium hexacyanoferrate (III), and sodium hydroxide, and heating for 3 min at 100 degrees C. Blue fluorescent spots were observed under ultraviolet light in a wavelength range of 250-400 nm. The detection limits for MDMA and the above related compounds were 50 ng. The proposed method was effectively applied to the detection of MDMA in urine samples.     

A real-time multiplex SNP melting assay to discriminate individuals

A method that quickly and inexpensively differentiates crime scene samples from multiple donors would expedite casework analysis by allowing the selection of probative items requiring comprehensive testing. This new method need not be perfectly definitive nor give a complete 13 locus short tandem repeat (STR) profile; it simply must be able to differentiate between most victim and suspect samples. We describe the development of multiplex, single nucleotide polymorphism (SNP), fluorescence resonance energy transfer-based real-time polymerase chain reaction (PCR) assays to fulfill this need. Dual probes, one fluorescently labeled and the other labeled with a quencher, are monitored during a melt analysis to reveal an increase in fluorescence, which allows the assessment of the two SNP alleles. Two alternate 6-plex assays (with and without gender determination) have been developed for the six-color RG6000 real-time instrument (Corbett Robotics, Inc.) and one seven SNP plus gender assay (performed as two 4-plex assays, one with gender the other without) have been developed for use in four/five color real-time instruments. This technique can discriminate between 95% and 99% of samples from different individuals. This assay is fast (approximately 2 h), much less expensive than STR analysis, and uses a real-time PCR instrument which is found in most forensic and molecular biology labs.     

论我国台湾地区“司法院”大法官解释两岸关系的方法

我国台湾地区"司法院"大法官凭借所谓"释宪"机制,在两岸关系发展中扮演着重要角色。其针对两岸关系,已作成"法统"型、权利型、制度型三类共16个解释。大法官在解释两岸关系时,综合运用了文义、论理、历史、体系等传统解释方法,以及"政治问题不审查"、"结果取向解释"以及"宪法解释宪法"等新兴解释方法。经过数十年的发展,大法官在选择解释两岸关系的方法时,表现出所谓"去政治化"倾向。而"台独"分裂势力也正是利用大法官解释两岸关系时的"去政治化"倾向,力图通过维持形式上"中立"的法学方法,为其"台独"目的提供"正当化"外衣。     

"一国两制"下人民内部矛盾问题初探

在“一国两制”实践中,人民内部矛盾出现了一些新情况、新问题。两种不同性质的经济基础、政治上层建筑、社会意识形态并存是人民内部新的经济、政治、思想文化矛盾的直接根源。其中两种不同性质经济基础并存是诸种矛盾最深刻的根源。必须坚持国家统一原则,“两制”长期共存、合理分权原则,用“一国两制”、发展经济、依法治国、沟通交流和宣传思想工作的方法,妥善处理“一国两制”实践中出现的人民内部矛盾。     

猪生殖与呼吸综合征病毒TaqMan荧光定量RT-PCR检测方法的建立

根据GenBank登录的PRRSV保守基因序列设计合成了引物和探针,并对其进行了筛选;对荧光定量PCR的反应条件进行了优化,建立了TaqMan荧光定量RT-PCR检测方法。同时用建立的检测方法对组织病料进行了检测,并与常规RT-PCR做了对比。结果显示,所建立的TaqMan荧光定量RT-PCR方法灵敏度可达5.0×100拷贝/μL,比常规RT-PCR灵敏度高100倍。用该方法对东莞、增城、湛江等地的猪血清和多种组织样品进行了检测。结果,该方法与常规RT-PCR检测方法的阳性符合率为100%。用该方法对3份不同的组织样品进行了重复检测,结果表明,该方法具有良好的重复性,可满足当前PRRS的诊断需要。     

论“三个代表”重要思想与“全面建设小康社会”目标

全面建设小康社会是社会主义中国确立的21初期新的发展战略.它是在探求"两大基本问题"和"两大规律"的进程中产生的丰硕成果,是对"三个代表"重要思想的最好实践和检验.     

知识产权与发展政策的整合

在我国以信息化带动工业化并最终实现工业化的进程中,要充分认识到知识产权制度的局限性,改变过去全盘采用外国技术的被动局面,在培育国内相关产业的同时,应支持本土厂商与外国公司竞技,直至假以必要的国家政策.     

海峡两岸继承法比较研究

两岸继承法除存在若干相似之处外 ,还有诸多差异。这些差异有的反映了中国大陆特色 ;有的属于大陆继承法上的漏洞 ;更多的则属于具体制度上的差异。研究这些差异对于完善大陆继承法 ,制定民法典继承编具有重要的参考价值。     

健全行政执法检察监督机制的路径探析

"穿透式"行政检察监督是指检察机关从监督法院行政审判活动穿透至监督行政机关作出的行政行为。为进一步推动检察机关做实行政检察工作,做到"一手托两家",促进依法行政,建议从"穿透式"行政检察监督面临的困境、监督的必要性及可行性、监督的范围及对象、监督的方式及手段、监督机制的构建等方面对狭义行政执法检察监督的定位与界限进行探索,从而为推进社会治理现代化进程提供可行的建议。     

在服务大局中彰显工会作为

推进＂两个普遍＂,发展和谐稳定新型劳动关系,是工会组织扩大覆盖面、增强凝聚力的现实需要。应积极争取更多资源和手段,谋求构建联动工作新格局;健全完善激励奖惩机制,推动＂两个普遍＂,实现全面科学发展;积极开展工资集体协商＂要约行动＂,确保＂两个普遍＂取得实效;通过加强培训,全面提高工会干部的能力与素质;坚持通过党建带工建、工建服务党建,搭建落实＂两个普遍＂的新载体。由于黑龙江省总工会采取的措施较为得力,所以其工会组建工作取得了较大较快的进展。