Acute effects of carbon monoxide and cyanide on hepatic mitochondrial function

The effects of carbon monoxide and cyanide on the hepatic redox state and energy charge were investigated. Rats were used for the experiment under pentobarbital anesthesia. Immediately after laparotomy, a rat was placed in an animal chamber made of a transparent plastic box and exposed to a test gas for 3 min. Every test gas was produced in a gas chamber connected to the animal chamber with a flexible tube. HCN was produced from NaCN and H2SO4. In the CO inhalation experiment, various amounts of CO were introduced into the gas chamber. Immediately after an exposure, about 2 g liver was frozen in situ with a precooled clamp. Oozed blood from the wound surface was sampled. Concentrations of ATP, ADP, AMP, acetoacetate, and beta-hydroxybutyrate in hepatic mitochondria were determined, and the redox state and the energy charge were calculated. For cyanide as well as CO, significant negative correlations were found between the concentration in the blood and the redox state. The same held true for the energy charge. The redox state showed a slight increase at low concentrations of both gases; however, thereafter it began to decrease sharply with increases in concentrations. When concentrations of the toxicant in the blood reached certain levels, a kind of turning point, beyond which the redox state does not decrease any more, was observed. It was about 40% for HbCO and about 2.0 micrograms/ml for cyanide, and the points seemed to be related to the concentrations, beyond which cells are irreversibly damaged. On the other hand, the energy charge did not change at low concentrations. With an increase in toxicant concentrations, the energy charge decreased drastically. The rate of decrease in the energy charge became higher when blood concentrations exceeded certain levels. It was about 40% for HbCO and 2.0 micrograms/ml for cyanide. The presence of low levels of blood cyanide did not affect the relationship between the energy charge and the HbCO concentration.     

A City‐wide Investigation of the Isotopic Distribution and Source of Tap Waters for Forensic Human Geolocation Ground‐truthing

Human geolocation is prefaced on the accuracy of the geographic precision of mapped isotopic values for drinking water. As most people live in cities, it becomes important to understand city water supplies and how the isotopic values uniquely reflect that city. This study investigated the isotopic distribution of δ²H and δ¹⁸O from sourced tap waters that were collected from across the Metro Vancouver (MV) area (n = 135). The results revealed that the isotopic values reflect their water sources with a range of 5.3‰ for δ¹⁸O_tap and 29.3‰ for δ²H_tap for MV. The results indicate that individual cities need higher resolution studies to determine their tap water isotopic ranges, and a good understanding of the water supply network itself for human geolocation work. With an extended high‐resolution understanding of each city, human tissue may be compared with more certainty for geolocation.     

The demographic and agricultural development of the Kokand oasis during the Russian Imperial era: nomad immigration and cotton monoculture

ABSTRACT

This study investigates oasis expansion in the precolonial period and agricultural evolution in the colonial period using village-level statistics from the early twentieth century. This survey illustrates that the Kokand oasis in the Ferghana Valley initially appeared in the central part, where the Sart population settled by the seventeenth century. In the eighteenth and nineteenth centuries, the Uzbek and Karakalpak tribes migrated to the valley peripheries, while the Kyrgyz semi-nomads settled in the alluvial fan. It is not surprising that such a migratory process created a mosaic-like ethnic distribution. Furthermore, this study suggests that the pattern coincided with a variety of agricultural practices, and presents two contrasting models of cotton monoculture under the Russian Empire. The main area occupied by the Sarts presents a general model of canal-irrigated cotton planting in Central Asia, while the Karakalpaks’ cotton planting using groundwater in the periphery suggests multiple courses of nomad sedentarization.     

Rising giants: Development in China and India

Can economic growth turn overpopulation from a liability to an asset? China and India tantalize investors as the “last frontiers” of emerging consumer markets; together, they hold 37 percent of the world's 5.7 billion people. Despite their many contrasts, China and India face similiar, immense development hurdles. Mired in economic stagnation and poverty, both countries undertook economic reforms that gained momentum in the 1980s. In this comparative study, IIPS Senior Research Fellow Eimon Ueda analyzes their ongoing economic liberalization efforts and prospects. Ueda comes to IIPS from the Ministry of Finance and has served at the Japanese Embassy in India.     

Paired stable carbon and oxygen isotope analyses of human enamel for forensic human geolocation: An exploratory study

Stable carbon isotope analysis has proven utility for reconstructing dietary information in humans in past populations. The usefulness of stable carbon for forensic geolocation has been little investigated, largely because of the globalization of the human diet seemingly rendering it inconsequential. This study queried this assumption at a country-wide level on a known sample group. Stable carbon isotope values were obtained from human enamel with known biographical and geographical information to determine whether stable carbon, when paired with stable oxygen isotope values, could differentiate Canadians from non-Canadians. Samples originating outside of Canada were separated into three regions, and a linear discriminant analysis was used to generate discriminant functions that best separate the regions according to the stable carbon and oxygen isotope values. The results revealed two functions, where the first function explained 92.1% and the second 7.9% of the variance. Although some overlap in stable carbon and oxygen values was observed for individuals from both the United States and Canada, differences were observed between those from Canada and other geographical regions. This study demonstrated that pairing the dietary isotope carbon with the geolocator stable oxygen isotope, produced an interesting separation geographically, one that might well be helpful when attempting a geolocation query on unknown human remains.     

Potential risk and promotive factors for serious delinquency in Japanese female youth

In Japan, concerns toward contemporary youth offending and violence have resulted in multiple changes in the treatment of youth. These changes are the result of negative societal perceptions of youth following a string of violent youth-on-youth murders and media sensationalizing juvenile crimes. Compared to what is known about male criminality, very little is known about female criminality, particularly about important risk and promotive factors for delinquency. Using the risk factor prevention paradigm, the study investigates potential risk and promotive factors for delinquency in 219 Osaka female youths, aged 15–18. The findings show that the strongest risk factors were high risk-taking and having troubled peers, whereas having a nonworking mother had promotive effects. Females were also compared to males, and the only significantly different factor was having a nonworking mother.