Effect of toluene inhalation on astrocytes and neurotrophic factor in rat brain

Toluene, an abused substance in Japan, is a neurotoxic chemical that has been shown to have neurobehavioral and electrophysiological effects. In previous work, both acute and chronic effects of toluene on cells have been studied extensively. However, although glial cells are thought to play an important role in the survival of neurons in the brain, the effect of toluene on glial cell function has not yet been characterized. To elucidate this, the effect of toluene inhalation on astrocytes in rat brain was examined. Toluene exposure (1500 ppm for 4 h on 4-10 days) augmented glial fibrillary acidic protein (GFAP) immunoreactivity, particularly in the hippocampus and cerebellum. Quantitative analysis showed that toluene inhalation markedly enhanced GFAP expression in the hippocampus and cerebellum. In both regions, proliferating cell nuclear antigen (PCNA) showed no obvious changes, but glutamine synthetase (GS)-immunoreactive cells were markedly increased by toluene exposure. Thus, the elevation of GFAP expression was induced by astrocyte activation rather than by cell proliferation. If toluene exposure activates astrocytes, astrocytes may play a role in the neurophysiological changes observed in toluene intoxication. A neurotrophic factor, basic fibroblast growth factor (b-FGF) was observed immunohistochemically in the capillary vessel walls in the hippocampus and the cerebellum of toluene-intoxicated rats. Basic-FGF may have induced GFAP expression both in the hippocampus and the cerebellum. So, other neurotrophic factors may affect the difference of GFAP elevation between the hippocampus and the cerebellum. These differences may relate to neurobehavioral function of each brain part after toluene exposure.     

The generic drug market in Japan: will it finally take off?

Historically, brand-name pharmaceuticals have enjoyed long periods of market exclusivity in Japan, given the limited use of generics after patent expiration. To improve the efficiency of the health-care system, however, the government has recently implemented various policies aimed at increasing generic substitution. Although this has created expectations that the Japanese generic drug market may finally take off, to date, generic usage has increased only modestly. After reviewing the incentives of key market participants to choose generics, we argue that previous government policies did not provide proper incentives for pharmacies to boost generic substitution. We offer some recommendations that may help to increase generic usage.     

Prime Minister Nakasone and The United States of America

Although it is commonly believed that as Prime Minister of Japan, Nakasone Yasuhiro focused diplomatic efforts on the United States, his first trip abroad as prime minister was to the Republic of Korea. In the 1950s, he was even a vocal critic of the US-Japan alliance. But he was different from many other politicians in reading books, traveling abroad to discuss issues with foreign leaders, and soliciting ideas and advice from intellectuals. In the end, he acquired mature views on how to fight the Cold War as well as maintain robust relations with the US. This article traces the evolving approach he had with the United States from the Occupation through the “Ron-Yasu” relationship during his prime ministership.     

The Dynamic Interdependence of Developmental Domains Across Emerging Adulthood

Emerging adulthood is a period in which profound role changes take place across a number of life domains including finance, romance, and residence. On the basis of dynamic systems theory, change in one domain should be related to change in another domain, because the concept of development according to this approach is a relational one. To evaluate this hypothesis dynamic systems analysis was applied to data from narrative interviews of 200 respondents covering the years between 17 and 27 to examine how change in one domain affects change in another domain. In each dyad, the fit of the model significantly deteriorated when the coupling between domains was removed providing support for the assumption of interdependency. On average, assuming greater responsibility in one domain was associated with assuming greater responsibility in the other domain. However, imbalances were also observed in which role assumption in one domain far exceeded role assumption in another domain. These imbalances can have detrimental effects and indicate the utility of a balanced approach to development. The findings underscore the importance of studying the relational unit between domains, which is critical to understanding development over time within domains. Post-Doctoral Research fellow in Geriatric Neuro-Psychiatry at Columbia University, College of Physicians and Surgeons and the New York State Psychiatric Institute. He received his PhD in Clinical Psychology at the University of Massachusetts Amherst and his major research interests include statistical methods for evaluating psychiatric nosology, late-life depression, and the stability and change of personality and personality disorders across the lifespan. Research Scientist at the University of Virginia. He received his PhD in Quantitative Psychology at the University of Virginia. His primary interests are in longitudinal data and applying dynamical concepts to structural equation modeling and multilevel methodology. Professor of Psychology at the University of Southern California. He received his PhD in Psychology at Hofstra University. He is principal investigator of the NIA funded National Growth and Change Study and has primary interests in the dynamics of cognitive ability over life span with special emphasis on longitudinal research designs, methodology, and statistical modeling. Research Scientist at New York State Psychiatric Institute and Professor of Public Health (Epidemiology) in Psychiatry at Columbia University College of Physicians and Surgeons and Columbia University School of Public Health. She received her PhD in social psychology at New York University. Major interests include quantitative methodology and statistics, developmental psychopathology, and lifespan development. Assistant Professor of Psychiatry at Columbia University College of Physicians and Surgeons. He received his M.D. and Ph.D. in Biostatistics in China. His research interests include epidemiological studies, quality of life, and multivariate statistical methods.     

A demonstration of spermatozoa on vaginal swabs after complete destruction of the vaginal cell deposits

The proteolytic enzyme, proteinase K, has been found to destroy all vaginal cells though it does not have the same effect on spermatozoa. In cases of sexual offenses, in which a swab has been used to wipe out the vagina, the female cells and their nuclei on that swab may also contain the heads of spermatozoa. After as short a time as 30 min of proteinase K treatment, the spermatozoa that had separated from the enzymatically destroyed vaginal cells were recovered. This proteinase destruction furnishes some spermatozoa with deformed heads and a somewhat greater number of isolated tails though a sufficient number of spermatozoan heads still remain for a reliable diagnosis. For detection of spermatozoa from a vaginal swab after proteinase K pretreatment, the heads of the spermatozoa are distinctly stained by Oppitz's method. Further, on prior treatment with proteinase K, the ABO blood grouping of the spermatozoa could also be determined on the vaginal swab by using the absorption-elution technique. The resistance of the spermatozoa to proteinase K is the basis for this method.     

Changes in the specificity of blood groups induced by enzymes from soil fungi

Three strains of Cunninghamella elegance (C. elegance), Penicillium simplicissimum (P. simpl.), and Aspergillus niger (A. niger) were isolated from soil that produced the enzymes acting on blood groups A and B red cells. Culture filtrates from these fungi contained alpha-N-acetyl-D-galactosaminidase as an A-decomposing enzyme, which resulted in an almost complete loss of A specificity and an enhancement of blood group H activity as measured by elution tests using monoclonal antibodies. They also contained an alpha-D-galactosidase and an alpha-L-fucosidase, which partially destroyed the blood group B specific activity, but did not influence the blood group H specific activity.     

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,

Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.     

Distributing Responsibility for Wrongdoing Inside Corporate Hierarchies: Public Judgments in Three Societies

The decision rules individuals use to judge wrongdoing committed inside corporations and other hierarchical organizations are not well understood. We explore this issue by asking random samples of individuals in Moscow, Tokyo, and Washington, D. C., to respond to four short vignettes describing acts of wrongdoing by people in corporations. The vignettes are experiments that manipulate the actor's mental state, the actor's position in the organization, and whether the actor's decision was influenced by others in the organization. We examine (1) the distribution of responsibility among people in the organization, (2) how individual responsibility affects the attribution of responsibility to the organization itself, and (3) cross-national differences in attributions. We find that both what the actors did (their deeds) and the position they occupied (their roles) significantly influence the responsibility attributed to them. The responsibility attributed to the organizations themselves is a function of the responsibility attributed to the actors inside the organization, but not a function of the independent variables in the experiments. Cross-national differences emerge with respect to the responsibility assigned both to individuals and to the organizations themselves. We discuss implications of these results for past and future work.     

Detection of Lea substance in saliva stains by enzyme-linked immunosorbent assay (ELISA) using anti-gum arabic serum

It is known that rabbit anti-gum arabic (GA) serum has cross-reactivity with Lea antigen, and that, by using this cross-reactive anti-Lea antibody, the presence of Lea antigen in red blood cells and saliva can be demonstrated with accuracy. We have devised a rapid and highly sensitive method for detecting Lea substance in human saliva by the enzyme-linked immunosorbent assay (ELISA) method using an anti-Lea antibody isolated from anti-GA serum by affinity chromatography on Synsorb Lea. The ELISA plate, coated with the specific anti-Lea antibody, adsorbed the Lea substance in saliva which was subsequently identified by adding enzyme labeled anti-Lea IgG in that order. The method could detect the Lea substance in Le(a+) saliva stains as small as 0.1 by 0.1 cm in size that had been stored at room temperature for three weeks and in Le(a+) saliva stains 0.7 by 0.7 cm in size that had been stored for ten years. This method seems to be useful for quantitative analyses of the Lea substance in various body fluids.