Analysis of STR loci in Cartagena, a Caribbean city of Colombia

Allele frequencies, together with some parameters of forensic interest were estimated for nine STRs included in the AmpF/STR Profiler kit (CSF1PO, D3S1358, D5S818, D7S820, D13S317, FGA, TH01, TPOX and vWA) in a sample of 215 unrelated individuals from Cartagena (Colombia). For all loci, no significant deviations from Hardy-Weinberg equilibrium were observed. Comparative analysis results between our data and those from other Colombian and African population samples revealed significant differences, except with two Colombian Caribbean Coast sub-regions.     

Analysis of Δ-tetrahydrocannabinol in oral fluid samples using solid-phase extraction and high-performance liquid chromatography–electrospray ionization mass spectrometry

An analytical method using solid-phase extraction (SPE) and high-performance liquid chromatography–mass spectrometry (LC–MS) has been developed and validated for the confirmation of Δ⁹-tetrahydrocannabinol (THC) in oral fluid samples. Oral fluid was extracted using Bond Elut LRC-Certify solid-phase extraction columns (10 cm³, 300 mg) and elution performed with n-hexane/ethyl acetate. Quantitation made use of the selected ion-recording mode (SIR) using the most abundant characteristic ion [THC + H⁺], m/z 315.31 and the fragment ion, m/z 193.13 for confirmation, and m/z 318.00 for the protonated internal standard, [d₃-THC + H⁺]. The method proved to be precise for THC, in terms of both intra-day and inter-day analyses, with coefficients of variation less than 10%, and the calculated extraction efficiencies for THC ranged from 76 to 83%. Calibration standards spiked with THC between 2 and 100 ng/mL showed a linear relationship (r² = 0.999). The method presented was applied to the oral fluid samples taken from the volunteers during the largest music event in Portugal, named Rock in Rio-Lisboa. Oral fluid was collected from 40 persons by expectoration and with Salivette^®. In 55% of the samples obtained by expectorating, THC was detected with concentration ranges from 1033 to 6552 ng/mL and in 45% of cases THC was detected at concentrations between 51 and 937 ng/mL. However, using Salivette^® collection, 26 of the 40 cases had an undetectable THC.     

Unarmed Utopia Revisited: The Resurgence of Left-of-Centre Politics in Latin America

This paper seeks to examine the extent to which left-wing forces are making a comeback in Latin America and to draw out the political implications of their political ascendancy. It argues that while left-of-centre parties have developed a persuasive critique of the failures of liberal democracy and economic neoliberalism in the region, there is as yet no conceptual clarity or distinct policy initiatives materialising from the left's promises of deepening democracy and implementing an alternative economic model. When in power, left-of-centre parties have followed a strategy of 'bending and moulding' existing political institutions and the free-market economic model rather than attempting radical political and economic reforms. This paper concludes that left-of-centre parties are right in accepting that there is little room in the region for an anti-systemic model and that instead the emphasis should be placed on making states, markets and democracy work better to secure development, address social demands and attack the root causes of discrimination and inequality. But leaving behind old certainties and adapting to the new political and economic environment has come at the cost of a loss of intellectual confidence, ideological clarity and weakened identities.     

The contribution of the right to information laws in Europe to local government transparency on sustainability

The aim of this paper is to examine the influence of the right to information laws on sustainability transparency in European local governments. This goal is novel, in that previous studies have examined the effects of various factors on the dissemination of government information (demographic, socioeconomic, political and financial) but not the contribution of legal factors to online transparency on environmental, social and economic sustainability. Our research question is this: Do information laws contribute to transparency on sustainability? Using the Global Reporting Initiative guidelines and a statistical regression analysis, we studied the websites of 106 local governments in ten European countries. The results obtained show that when transparency laws clearly stipulate the rules applicable and the procedures established for appeals, exceptions, refusals and requests, this can favour transparency on environmental, social and economic sustainability. Our findings advance understanding of this field and reinforce the basis for legal reforms to enhance sustainability transparency.

     

On the Move

正Moving house is traumatic at the best of times.I recently decided to look for a new apartment on the eastern side of Beijing and seek greener pastures compared with my present abode in the back of beyond.Previously,colleagues had always helped me to hunt down that elusive place to hang my hat.With little time at my disposal to physically hunt down apartments on my own,I searched for that most persuasive of     

Parties, Democracy and Grounded Utopias: A Reply to Sara Motta

The article is a reply to Sara Motta's article 'Utopias Re-imagined: A Reply to Panizza' in this journal. It discusses the relations between representative and participatory democracy in Latin America in the light of Motta's vindication of different forms of participatory democracy. It argues that when analysing the advances of the left and the centre-left in contemporary Latin America it is difficult to ignore the strategic role played by left-of-centre political parties in winning elections and the importance of controlling the state as a crucial instrument for promoting change. It further argues that while participatory democracy is essential for a democratic polity, it presupposes a well-functioning representative democracy rather than an alternative to it. Against Motta's celebration of localised, anti-capitalist utopias the article vindicates a process of iterative yet cumulative change that shapes and reshapes the political and institutional parameters that redefine what governments consider politically possible, feasible and desirable. It concludes by noting that the twentieth century's failure of totalising utopias makes us overlook the success of other, more grounded and open forms of utopian thinking, such as political democracy and economic social democracy, which have the potential to improve the lives of millions of people in Latin America.     

DNA typing: an accessory evidence in doping control

A clear positive case for anabolic steroids doping was confounded by alleged urine tampering during doping control procedures. Review of the chain of custody showed no flaws, but nevertheless the athlete was adamant that the urine sample should be analyzed for DNA in order to support her contention that she was not the donor of the sample. The results obtained showed that the urine sample that scored positive for steroids contained nuclear DNA that could not be matched to the DNA obtained from the athlete's blood. On the other hand, the same urine sample contained mitochondrial DNA whose nucleotide sequences spanning the hyper variable regions HV1 and HV2 proved to be identical to those determined in mitochondrial DNA amplified from the athlete's blood. The occurrence of an extraneous genotype is compatible with exogenous nuclear DNA admixture to the athlete's urine. Alternatively, taking in consideration the mitochondrial DNA, we could not exclude that a sibling or a maternal relative of the athlete could have acted as a donor of the urine utilized for doping control and DNA analysis. Both situations point to possible tampering of the urine by the athlete. Adjudication at CAS maintained previous national and international federation decision that there was no proof of a chain of custody flaw to justify the athlete's allegation of urine substitution after collection.     

Age patterns of suicide: identification and characterization of European clusters and trends

Relatively few studies have addressed age patterns of suicide using specifically designed and systematized approaches. Our main objective was to identify and characterize age patterns of suicide in Europe, both on cross-sectional and longitudinal perspectives, based on data comprising the two last decades of the 20th century. A second objective was to determine whether some analytical methods could be useful as complementary approaches to the traditional examination of age-specific rate curves. We identified and characterized four patterns: upward-sloping, convex, downward-sloping, and uniform. The first three reproduce patterns previously described, though we propose some modifications to the respective classification criteria. Longitudinally, pattern stability was found for most countries, but some notable exceptions were detected. From the computation of multiple-decrement life tables by European country, period, and gender, we derived cumulative distributions of age at death for suicide. The results indicate that this methodology is a useful tool, not only for describing and summarizing information, but, more importantly, for providing a better understanding of intrinsic features and complexities of the patterns. Finally, we discuss practical implications of the study in the context of previous theories linking age patterns of suicide to sociological and economic dimensions, and we raise a few questions and hypotheses to be addressed in future research.     

Is Brazil becoming a “boring” country?

The article looks at the condition of Brazil's political system in light of the 1998 electoral results. It critically examines arguments that electoral volatility, political fragmentation and weak institutionalisation have produced a highly unstable political system unsuitable for sound policy‐making and processing change. While not underestimating the influence of electoral rules, it argues that given the combination of political, institutional and economic turmoil of the 1980s, it is not surprising that electoral volatility over the period was amongst the highest in the world. Arguably, however, these factors are now having a diminished impact on the political system, lessening volatility and allowing the emergence of a more institutionalised party system.