Y-chromosome STR system, Y-PLEX 12, for forensic casework: development and validation

The Y-PLEX 12 system, developed for use in human identification, enables simultaneous amplification of eleven polymorphic short tandem repeat (STR) loci, namely DYS392, DYS390, DYS385 a/b, DYS393, DYS389I, DYS391, DYS389II, DYS 19, DYS439 and DYS438, residing on the Y chromosome and Amelogenin. Amelogenin provides results for gender identification and serves as internal control for PCR. The validation studies were performed according to the DNA Advisory Board's (DAB) Quality Assurance Standards. The minimal sensitivity of the Y-PLEX 12 system was 0.1 ng of male DNA. The mean stutter values ranged between 3.76-15.72%. A full male profile was observed in mixture samples containing 0.5 ng of male DNA and up to 400 ng of female DNA. Amelogenin did not adversely affect the amplification of Y-STRs in mixture samples containing male and female DNA. The primers for the Y-STR loci present in Y-PLEX 12 are specific for human DNA and some higher primates. None of the primate samples tested provided a complete profile at all 11 Y-STR loci amplified with the Y-PLEX 12 system. Y-PLEX 12 is a sensitive, valid, reliable, and robust multiplex system for forensic analysis, and it can be used in human forensic and male lineage identification cases.     

Analyzing and Interpreting Lime Burials from the Spanish Civil War (1936–1939): A Case Study from La Carcavilla Cemetery

Over 500 victims of the Spanish Civil War (1936–1939) were buried in the cemetery of La Carcavilla (Palencia, Spain). White material, observed in several burials, was analyzed with Raman spectroscopy and powder XRD, and confirmed to be lime. Archaeological findings at La Carcavilla's cemetery show that the application of lime was used in an organized way, mostly associated with coffinless interments of victims of Francoist repression. In burials with a lime cast, observations made it possible to draw conclusions regarding the presence of soft tissue at the moment of deposition, the sequence of events, and the presence of clothing and other evidence. This study illustrates the importance of analyzing a burial within the depositional environment and taphonomic context.     

Sustainable citizenship for a technological world: negotiating deliberative dialectics

Incorporating the notion of sustainability is the biggest challenge for citizenship in a technological era. Existing conceptions of citizenship have not been able to grapple with compounded ecological, economic, cultural, and moral threats facing modern technology-infused societies. Nor has increased public participation, engagement, and dialogue resolved polarized positions on issues such as what constitutes quality of life or what is meant by the integrity of nature. This paper draws on the scholarship of both sustainability and citizenship to propose a framework of sustainable citizenship that seeks to emphasize shared values through a deliberated clash of ideas. Such a framework involves a negotiation of the dialectics of rights and responsibilities, state and non-state, public and private, human and non-human nature, universal and particular, and democracy and capitalism. The paper illustrates how sustainable citizenship can be applied to deal with contentious political and policy issues of new and emerging technologies.     

Social repair in divided societies: integrating a coexistence lens into transitional justice

This paper explores the importance of integrating a coexistence lens into transitional justice theory and practice. Transitional justice seeks to address a legacy of large-scale past abuses. In societies that have suffered from violent intergroup conflict, this legacy includes divided communities and widespread distrust and fear of the ‘other’. Transitional justice processes and mechanisms, however, are unlikely to repair intergroup relationships, transform communities or eliminate tensions in the absence of specific attention to promote coexistence. The field of coexistence provides a framework for thinking about intergroup relations and interdependence. Coexistence initiatives—such as dialogue facilitation, intergroup projects and associations aimed at achieving shared goals, and media campaigns designed to reframe the ‘other’—are essential to restoring trust, transforming perceptions and rebuilding relationships. Looking at countries that have experienced violent intergroup conflict, in particular Bosnia and Herzegovina, the paper provides examples of coexistence initiatives that have achieved successes in changing attitudes, repairing relationships and strengthening communities—and discusses their potential to contribute to transitional justice processes and mechanisms.     

Utility of the Y-STR typing systems Y-PLEX 6 and Y-PLEX 5 in forensic casework and 11 Y-STR haplotype database for three major population groups in the United States

The Y-PLEX 6 and Y-PLEX 5 systems enable analysis for 11 Y-STR loci. We present here the utility of these systems in forensic casework. A total of 188 samples, including 127 evidence samples, were analyzed using either or both of the systems. The evidence sample types included fingernail scrapings, sperm or seminal fluid, epithelial cells, blood and other tissues. The Y-STR typing systems provided useful probative results in difficult cases. A reference database for Caucasian (n = 517), African American (n = 535), and Hispanic (n = 245) population groups within the United States was generated for estimating the haplotype frequency in forensic casework. Among the individuals profiled, 311 Caucasians, 412 African Americans, and 194 Hispanics provided unique profiles in their respective population datasets. This is the first report describing the haplotype database for the set of 11 Y-STR loci recommended by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Linkage analysis reveals that the frequencies from forensically important autosomal loci can be multiplied with the Y-STR haplotype frequency. The results from Y-PLEX systems have been accepted in courts in the United States.     

‘Women of substance’: Commodification and fetishization in contemporary advertising within the Indian ‘Urbanscape'

Advertising today has become an intrinsic part of modern culture. Apart from the obvious and tangible extolment of the virtues of a product and/or brand, more insiduously and less tangibly, advertising plays a key role in the functioning of social attitudes and values. This paper examines how advertising discourses in India, in particular the hugely successful and representative Woman of Substance’ ad campaign in the magazine Femina, transforms the manifold attitudes and qualities of the woman into commodities to be marketed; and how the ad campaign positions the woman within the nexus of her social interactions. The paper traces the economic and socio‐cultural changes that have characterised India's transition towards an open market economy to situate the role that advertising discourse plays in urban India today in the representation of the ‘Woman of Substance’. These changes have occurred in a relatively short space of time and have interacted profoundly with advertising discourse. This can best be seen in the fact that an urbanising trend in India has diversified the range of approved cultural models available to women in the search for what has come to be fashionably referred to in recent times as ‘lifestyles‘— exemplified both in the ‘Woman of Substance’ ad campaign, and the magazine itself, Femina.     

The political ecology of traditional farming practices in Thana district,Maharashtra (India)

This article attempts to discuss the traditional agricultural practices dalhi and rab, in Thana district, Maharashtra (India): in 1818, when the British took over the district; the 1880s, when the effects of the British land revenue and forest policies were visible; and the 1980s. It is suggested that the traditional system was appropriate, in the past, given the existing ecology and the level of development of technology; and that even now a well‐regulated utilisation of forest resources for rab may be sound, in the long run, from the viewpoint of preservation of soil fertility and a pollution‐free environment, especially in the absence of viable alternatives.