Genetic data on 10 STR loci a population of western Poland

Allele frequencies for 10 STRs included in the AmpFlSTR SGM Plus kit were determined in a population sample of 668 unrelated individuals living in western Poland. All loci met Hardy-Weinberg expectations. Exact tests disequilibrium analysis revealed two departures from independence out of 45 pairwise comparisons. The combined matching probability (MP) and power of exclusion (PE) for all 15 loci are 2.56 x 10(-13) and 0.99996, respectively.     

Typeability of AmpFlSTR SGM Plus loci in brain and thyroid gland tissue samples incubated in different environments

Autolysis and putrefaction are crucial factors responsible for degradation of cells, tissues, and organs. Postmortem changes may assume different course depending on extrinsic and intrinsic conditions. The aim of the study was assessment of environmental effect on typeability of AmpFlSTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA, and gender marker amelogenin. Brain and thyroid gland tissue specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C in different environmental conditions. DNA was extracted by organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. A fast decrease in typeability rate was seen in specimens incubated in peat soil and in sand. Brain tissue samples were typeable in all AmpFlSTR SGM Plus loci within 126 days of incubation at 4 degrees C. Faster DNA degradation was recorded in thyroid gland specimens. In samples with negative genotyping results, no DNA was found by fluorometric quantitiation.     

COMPETITION IN FREIGHT RAILWAYS: "ABOVE-THE-RAIL" OPERATORS IN CENTRAL EUROPE AND RUSSIA

The railways of Russia and the Central and Eastern Europeancountries are in the process of restructuring. In most cases,the "vertical separation" reform model is being pursued, andreformers are seeking to introduce competition among freighttrain operators through the provision of "open access" to themonopoly infrastructure. This paper shows that, in two countries,Poland and Romania, a good deal of competition has been createdby the entry of new freight operators, many of them either largeshippers integrating upstream or former freight forwarders.However, in other countries, the incumbent freight operatorsretain virtually 100 percent of the market. In particular, Russiahas taken only the very first steps toward creating competitionin this sector, and new freight train operators face significantbarriers to competing with the incumbent.     

1,2,4‐Triazine‐Based Chromogenic Reagents for the Detection of Microtraces of Various Metals Left on Human Skin*

Abstract: This article extends the application of 1,2,4‐triazine‐based chromogenic reagents to the detection of nonferrous metal traces left on contact with canvas and human skin. The possibility of detection of iron traces resulting from contact with objects made of stainless steel was investigated as well. Additionally, the ability of triazines to form chromatic complexes with Zn²⁺, Ni²⁺, Co²⁺, Cu²⁺, Cr³⁺, and Al³⁺ ions was studied spectrophotometrically. Molar absorption coefficients, ranging from 8.8 to 29.9 × 10³/M/cm, provide high sensitivity of 1,2,4‐triazines toward nonferrous ions, thus, enabling the detection at concentrations as low as a few μM. The method was sensitive enough to detect traces resulting from a 1‐min contact with a stainless steel made object, which is commonly considered as a corrosion‐resistant material. The amounts of metal ions transferred to the skin after a 2‐min contact with objects made of brass, zinc, and copper were sufficient to develop chromatic imprints.     

Transfer of Triazine‐iron(II) Chromic Complexes Left by Iron Items on Textile Background and Human Skin

Abstract: The research is focused on the detection and transfer of iron traces left by iron items on clothing and human skin. The method is based on the formation of colored complexes between ferrous ions and five synthesized, mostly new triazines. Iron traces originally were left by iron rings on slightly wetted (artificial sweat) cotton fabrics and subsequently transferred to a separate textile substrate. Prior to the use of trazines the contact spots were treated with a new inorganic reducing agent (Sn²⁺) to reduce Fe³⁺ to Fe²⁺. The method is sensitive to detect iron traces on wetted canvas after 10 min contact with iron items. More spectacular results were obtained for traces left on human palm even after very short contact (10 sec). The new iron‐trace‐transfer method eliminated the contact of triazines solutions with human skin. Transmission visible spectra of Fe(II)–triazine complexes were determined.     

STR data for the AmpFlSTR SGM Plus loci from Warmia and Mazury (NE Poland)

Allele frequencies for the ten STRs included in the AmpFlSTR SGM Plus kit were determined in a sample of 320 unrelated individuals born in the region of Warmia and Mazury (NE Poland). All loci met Hardy-Weinberg expectations. Exact tests disequilibrium analysis revealed only one departure from independence out of 45 pair-wise comparisons of the 10 loci.