Use of the "SMITEST" PSA card to identify the presence of prostate-specific antigen in semen and male urine

To determine whether the prostate-specific antigen (PSA) could be identified in semen using the "SMITEST" PSA immunochromatographic membrane test card, we examined semen and other body fluids, including urine. Although PSA activity was detected in semen with high sensitivity using the "SMITEST" PSA card, it was also detected in adult male urine. However, the lower detectable limit in the urine was 1000-fold lower than that in semen. The concentration of PSA in adult male urine was found to be 800 ng/ml using the card. PSA activity usually can be detected in urine of individuals over 14 years old and it has been detected in urine from children as young as 11 years old. Therefore, the appearance of PSA in urine may occur anytime between the age of 12 and 14 years. To determine the stability of PSA activity in urine, dried samples of urine on filter paper were kept at room temperature for up to 3 years. Although the immunoreactive line showing PSA activity became weak after storage, it was still detectable, but faint, after 3 years. In addition, PSA activity was not detected in male serum or saliva and in the urine from human females, male cats or male dogs using the PSA card. We conclude that the PSA card is useful for identification of PSA in both semen and adult male urine.     

The Impact of Rural Votes in Foreign Policies: The FTA Policies under the DPJ Government in Japan

The recent trend of bilateral free trade agreements (FTAs) has pressured the governments of many countries to make such arrangements with their trade partners. Since its foundation in 1998, the Democratic Party of Japan (DPJ) has advocated free trade policies, partly because the party was an urban-based party. Thus, many expected that, when the DPJ assumed power in 2009, it would implement free trade policies as it had promised in the past. However, the DPJ government failed to deliver on its promise after spending three and a half years in office. It contrasts sharply with the Korean government under the leadership of Lee Myung-bak, which managed to conclude FTAs with its major trade partners, including the United States and the European Union. Both governments' free trade policies faced strong opposition from the agricultural industry, as farmers in Japan and Korea lacked international competitiveness. What explains the reasons why the Japanese government has been struggling to implement its free trade policies, while its Korean counterpart succeeded in signing a number of FTAs? Focusing primarily on the case of Japan and using the Korean case as reference, this study tries to provide an explanation for this puzzle by analyzing the impact of rural votes in the policy-making process.     

Allele frequencies for 15 STR loci in Tibetan populations from Nepal

Samples from 105 unrelated healthy Sherpa in Namche Bazaar and 111 unrelated non-Sherpa in Kathmandu valley from Nepal were used to obtain allele frequency data for 15 short tandem repeat (STR) loci (CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D19S433, D21S11, FGA, TH01, TPOX and vWA) included in the AmpFLSTR Identifiler kit. No deviations from Hardy-Weinberg equilibrium were observed, but only after applying a Bonferroni correction in the case of D5S818 in the Sherpa population and D7S820 in the Kathmandu population. Genetic parameters of forensic interest were calculated and genetic differentiation between the two populations tested.