A mixed DNA profile controversy revisited

Semaan et al. (J Forensic Res, 2020, 11, 453) discuss a mock case “where eight different individuals [P₁ through P₈] could not be excluded in a mixed DNA analysis. Even though … expert DNA mixture analysis software was used.” Two of these are the true donors. The LRs reported are incorrect due to the incorrect entry of propositions into LRmix Studio. This forced the software to account for most of the alleles as drop-in, resulting in LRs 60–70 orders of magnitude larger than expected. P₁, P₂, P₄, P₅, and P₈ can be manually excluded using peak heights. This has relevance when using LRmix which does not use peak heights. We extend the work using the same two reference genotypes who were the true contributors as Semaan et al. (J Forensic Res, 2020, 11, 453). We simulate three two-donor mixtures with peak heights using these two genotypes and analyze using STRmix?. For the simulated 1:1 mixture, one of the non-donors’ LRs supported him being a contributor when no conditioning was used. When considered in combination with any other potential donors (i.e., with conditioning), this non-donor was correctly eliminated. For the 3:1 mixture, all results correctly supported that the non-donors were not contributors. The low-template 4:1 mixture LRs with no conditioning showed support for all eight profiles as donors. However, the results from pair-wise conditioning showed that only the two ground truth donors had LRs supporting that they were contributors to the mixture. We recommend the use of peak heights and conditioning profiles, as this allows better sensitivity and specificity even when the persons share many alleles.     

The 1996 Split of the Islamic Movement in Israel: Between the Holy Text and Israeli-Palestinian Context

This article aims to explain the causes and meaning of the formal split of the Islamic Movement in Israel into two factions—following the decision to participate in the elections to the fourteenth Knesset (Israeli parliament) on May 29, 1996—while locating these in a larger theoretical framework. This split resulted from a delicate combination of doctrinal-ideological controversies relating to secular electoral competition and historical-political-tactical controversies that are rooted in the Israeli-Palestinian context. Specifically, the split of the Islamic Movement in Israel derived from two interpretations of the Islamic belief: a more literal or concrete interpretation and a more abstract one.     

Comparison of four DNA extraction methods to extract DNA from cigarette butts collected in Lebanon

Cigarette butts collected from crime scenes represent valuable sources of DNA. However the extraction of the genetic material may deem challenging especially when different contaminants may compromise the integrity, quality, and quantity of DNA obtained. This study aims at comparing four extraction methods (Chelex-100, soaking + Chelex-100, Chelex-100?+?PK, and DNA IQ? System) with the intention of identifying the one with maximal recovery rate and profiling success. DNA was extracted using aforementioned four methods from 70 cigarette butts collected from sites across Lebanon. DNA was quantified by qPCR using TaqMan Quantifiler Kit on an Applied Biosystems 7300 SDS instrument and genotypes were obtained using the PowerPlex® 21 kit on an Applied Biosystems 3130 Genetic Analyser. The findings of this work showed that DNA extraction with Chelex-100?+?PK is preferred to the other three methods when seeking both, a high yield and the generation of maximal numbers of full profiles. The Chelex-100?+?PK method is simple, cost effective, and therefore suitable for routine cigarette butts case studies.     

Islamism, Nationalism, and Western Modernity: The Case of Iran and Palestine

The rise of Islamic politics in the Middle East, particularly since the Iranian revolution, is the most cited example that supposedly testifies to the “clean” separation between “Islam” and the “West.” In this essay, I argue that it is not Islamic movements and ideology that confirm this separation. Rather, it is their incorporation into the scheme of Western modernity, with its binary distinctions and evolutionary reading of history, which constructs this separation. Using examples from Iran and Palestine, I show how Islamic ideology indeed defies the basic premises of Western discourse on modernity, expose its limitations, and question the constitution of Islam and the West as allegedly distinct, even opposing, categories.