The typing of group-specific component (Gc protein) in human blood stains

It is known that the typing of group-specific component (Gc protein) in human blood stains is difficult since Gc protein of the extracts of blood stains migrates more anodally to the α₁-globulin region in agar-gel immunoelectrophoresis, while Gc protein in liquid blood normally migrates to the α₂-globulin region. We have reported that the Gc protein found in the α₁-region is the result of binding of actin to Gc protein (Shinomiya, K., Kimura, H., Yoshida, K., and Shinomiya, T., J. Biochem., 92 (1982) 1163–1171, which renders it difficult to determine the Gc-phenotypes in the blood stains. On the basis of the above findings, we developed the method of phenotyping the Gc protein of human blood stains by agar-gel immunoelectrophoresis. Since the binding activity of actin to Gc protein is lost after treatment with a high concentration of guanidine HCl, the extracts of blood stains were treated with 4 M guanidine HCl to dissociate Gc protein and actin and then dialyzed to remove guanidine HCl. By this method we are able to determine the phenotypes of Gc protein in blood stains. The method we have developed is a useful tool in the forensic laboratory.     

Japan as an Active Agent for Global Norms: The Political Dynamism Behind the Acceptance and Promotion of “Human Security”

Japan has shaped a distinct human security policy based on evolving policy preferences of successive domestic political leaders and the gradual assimilation of external norms into its own foreign policy. Independent experts have played a particularly significant role in advising Japanese policy elites on how human security could be used by Japan to become an “intellectual leader” within the United Nations and other relevant institutions. This article explores those processes that occurred in the early phase of norm acceptance on the part of key Japanese policy actors and change agents in Japan from the late 1990s through 2003. It argues that human security has served as an effective approach for Japan to establish itself as a more independent foreign policy actor in contemporary international politics.     

Application of aspartic acid racemization to forensic odontology: post mortem designation of age at death

The extent of racemization of aspartic acid in the dentin of healthy, carious and impacted teeth was used to define the ages of individuals at the time of their death. These determinations were performed with an error of +/- 4 years. This method holds the promise of simplifying procedures in, and extending the application of forensic odontology. The current error range of this method may be reduced by analyzing teeth of the same positional types from deceased individuals of known age. Using this method, it appears that post mortem preservation of up to 10 years has a negligible effect on the values determined for the age of individuals at death.     

Conflicts of decision-making processes between Clean Development Mechanism and project financing

Clean Development Mechanism (CDM) projects are believed to be financially unviable without additional revenues from CDM in the form of saleable certified emission credits; this is an important premise to be verified in the CDM approval process. However, in financing a CDM project, it is crucial for financiers and investors to ensure that the project is viable without CDM revenues. Thus, there are clear conflicts in the processes and priorities between the CDM approval in theory, and financing and investment decisions in practice. Considering the post-CDM frameworks, this article uses a case study of Bhutan’s Dagachhu hydropower project, the world’s first cross-border CDM project, to suggest practical lessons learnt.     

Illegal Route Estimation of the Seized Illicit Drug, Methamphetamine, by the Comparison of Striation Marks on Plastic Packaging Films

Abstract:  In Japan, the most common illicit drug is methamphetamine. It is possible to trace the origin of this drug by analyzing its organic and inorganic impurities and/or byproducts using several methods, such as GC, GC/MS, and inductively coupled plasma-mass spectrometry (ICP-MS). As reported here, one other method includes comparison of the striation lines of polymer sheet layers from packaging using a polarized light method. Other alternative methods include analyzing the heat sealer pattern, layer thickness surface characteristics, and/or components of polymer sheet layers using infrared spectroscopy. Several of these alternative methods were used to analyze the origins of 29 packages confiscated from three regions over a 1000 km distance in Japan. Results indicated that packages seized from different regions had some polymer sheet layers which contained striation lines and heat sealer patterns that were similar.     

Usefulness of dura mater in providing DNA samples for identifying cadavers

We examined the usefulness of the dura mater in identifying human remains. Dura mater was collected from 50 cadavers, including drowned, charred, and mummified remains. The STR genotype using the AmpFlSTR Identifiler Kit could be typed at 15 STR and amelogenin loci in 30 samples of 33 cases. Furthermore, the ABO genotype and amelogenin using gel-based methods could be typed in 44 samples of 50 cases. In cases with successful typing of STR, ABO-DNA, and amelogenin, the longest time after death was from 12 to 26 days in a drowned body. The minimum quantity of dura mater required for DNA extraction was about 2.5 mg, dried and fixed by ethanol, in a cadaver 15 h after death. The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification.     

Social capital,household income,and community development in Bhutan: a case study of a dairy cooperative

Using a case of a dairy cooperative in Bhutan, this study investigates how social capital enhances individual household income and community development in rural areas. It also examines the influence of cooperatives in social capital formation. The study finds that social capital facilitates households that are resource-constrained by working together to reduce costs through economies of scale, enhancing information accessibility, building confidence, and strengthening teamwork. It fosters not only an improvement in the livelihoods of cooperative members, but also provides a positive influence for non-members and the community as a whole. The study also reveals that cooperatives influence formation of three forms of bonding, bridging, and linking social capitals. While bonding social capital facilitates strong teamwork among the members, bridging and linking social capitals connect the cooperatives with the outside networks and provides them more opportunities and services for its sustainability.