The development of a test-system for the quantitative and qualitative evaluation of DNA content in criminalistic objects by the real-time polymerase chain reaction

An original test-system for the preliminary quantitative and qualitative evaluation of isolated DNA is proposed by the polymerase chain reaction in real time (PCR-RT) based on the TaqMan technology. This test-system permits to simultaneously measure the amount of DNA in the sample, identify the genetic gender, and detect PCR inhibitors. The method has been approbated in the practical work of forensic medical experts.     

Establishing the personal identity of the victims in aviation catastrophes

Algorithm of organization measures and expert investigations is proposed, based on experience gained in expert studies during liquidation of aircraft catastrophes. It permits effective classification, differentiation, and identification of victims and is based on traditional and high technological methods of investigation.     

Prospects for determining age from human soft tissues (a morphometric study)

Biometric studies of the aorta, endocrine glands (testes, prostate, and thyroid), and x-ray examination of the hands from 427 corpses of men and women aged 17-89 years helped formulate equations of paired and multiple linear regression for the diagnosis of age. The difference between biological and passport age is significant if the diagnosis is made on the basis of a single object or single sign. Complex use of several objects described by many signs is recommended; in this case the confidence intervals is no more than +/- 4.09 years.     

The detection of RhD antigen of the rhesus system by the real time polymerase chain reaction

A method for the detection of RhD using a DNA preparation and the newly-developed test-system based on the real time polymerase chain reaction is described. The study including a large variety of specimens has demonstrated the applicability of the novel system to forensic medical examination.