Investigation of the accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) in rat kidneys

The accumulation of 2,4-dichlorophenoxyacetic acid (2,4-D) and its metabolite 2,4-dichlorophenol (2,4-DCP) in the kidneys of rats was investigated. Male and female Sprague-Dawley rats were given 2,4-D in drinking water and food for 30 days. Group A (control group) was fed a normal diet, Group B was fed 50 ppm 2,4-D in 15 g food, Group C received 100 ppm 2,4-D in 15 g food, Group D received 25 ppm 2,4-D in 15 ml drinking water and Group E was given 50 ppm 2,4-D in 15 ml of drinking water. Levels of 2,4-D and 2,4-DCP in kidneys were determined using high performance liquid chromatography (HPLC). It was observed that at low doses of 2,4-D, the metabolite, 2,4-DCP found in the kidneys.     

Image analysis as an adjunct to sodium rhodizonate test in the evaluation of gunshot residues: an experimental study

It is important to investigate the clothing, as well as the body, to determine the range of fire of entry wounds in firearm injuries. Clothing can affect the amount of gunshot residues (GSR) reaching the body and their distribution. The amount and distribution of the GSR also vary according to the distance between the firearm and the target. Sodium rhodizonate test provides valuable data when clothing is available for examination. In the absence of clothing, light microscopic examinations may add additional information regarding the range of fire. In this study, a sodium rhodizonate test was done on 80 garment samples containing the bullet entrance. The 80 calfskin samples were processed histologically and were stained using Alizarin Red S. These were also evaluated with computer-assisted image analysis. Gross residues were seen on military camouflage clothing in samples from < or = 45-cm group. White flannel undershirts under the military camouflage contained rhodizonate-positive particles only around the contact wounds. With image analysis, however, the residues could be detected also in the skin samples of the 2.5-cm- and 5-cm-range groups. We suggest that the image analysis can be combined with other techniques and it can provide valuable data in the determination of entry wounds and also in the estimation of firing distance.     

An Evaluation of Childhood Deaths in Turkey Due to Yellow Phosphorus in Firecrackers

Yellow phosphorus (YP) is a powerful protoplasmic poison used in the manufacturing of matches, pest poisons, firecrackers, firework cracker, lights for watches, military ammunition, and agriculture fertilizer. YP is extremely flammable and toxic and easily absorbed from the gastrointestinal tract. In this study, we examined childhood deaths from 1997 to 2012 resulting from the ingestion of firecrackers. The patients ranged from 2 to 15 years of age and were admitted to the hospital with a variety of symptoms. Those that presented with nausea, vomiting, and hypotension rapidly deteriorated and entered a coma. An autopsy was performed in all but one of the 16 cases reviewed. Macroscopically, the livers had a yellowish discoloration with petechial bleeding. Histopathologic examination revealed acute toxic hepatitis. In conclusion, these firecrackers are found in corner shops throughout Turkey, may cause death in children with little warning, and should be banned to prevent further deaths.     

Female student athletes’ perceptions of acceptability and the occurrence of sexual-related behaviour by their coaches in India

The current study assessed the perceptions of acceptable sexual behaviour of coaches and the occurrence of sexual harassment among female student in India. A sample of 180 Indian female student-athletes at intercollegiate and inter-university levels with male coaches participated in this study. A questionnaire on sport-specific Touch and Behaviour versus Unwanted Intimacy from coaches (Vanden Auweele et al., 2008), consisting of 41 items on a 5-point rating scale was used. The participants were asked to indicate the acceptability of specific coaching behaviour as well as the occurrence of the behaviour represented in each item. The acceptability of the behaviour was determined by means and standard deviations, while the occurrence was determined by frequencies. Factor analysis was performed to determine the structure of the coaches’ behaviour and athletes’ acceptability thereof, which yielded four factors. Cronbach’s alpha was used to determine the internal consistency of the extracted items on each factor. Two factors, namely, unwanted sexual behaviour and inappropriate verbal and physical sexual behaviour, were regarded by athletes as very serious and unacceptable coach behaviour, while a third factor represented context-dependent suspicious behaviour (a grey area in which athletes differ in their opinion) and was perceived to be serious and unacceptable. A fourth factor represented acceptable behaviour. The occurrence of very serious and unacceptable behaviour was reported by 31% of the female athletes.     

Investigating demodex in forensic autopsy cases

Demodex is an ectoparasite living in the skin as a nonpathogen or a pathogen. It is also known that demodex acts as a vector of pathogenic microorganisms. In this study, we have investigated the rate of occurrence and vitality of demodex in forensic autopsies that have a high risk of contamination by infected organisms. The study, which was cross-sectional, conducted on 100 autopsy cases: 77 of the bodies were male and 23 female. The average age was 41. The samples were taken from the forehead, nose, cheeks, eyelashes and the temporal areas using the standardized skin surface biopsy and hair epilation techniques that were modified for this study. The data obtained were assessed statistically. Demodex was determined in 10% of all the cases. The cases revealed that demodex increased in old age and was more frequently seen in people with fair complexion. It was most frequently found in the forehead and the cheek. The longest postmortem interval in the positive cases was 55 h. The relationship between the postmortem interval and the presence of demodex was not statistically significant. Considering the fact that demodex, which is transmitted from human to human through skin contact, acts as a vector of pathogenic organisms and can stay alive in dead bodies for a long time, we think that the personnel performing autopsies without taking the necessary precautions are under risk.     

Arrhythmogenic right ventricular dysplasia cases in forensic autopsies

Arrhythmogenic right ventricular dysplasia or cardiomyopathy (ARVD or ARVC) is an increasingly recognized entity with clinical and forensic implications. This is the first series documenting ARVD in 5 separate cases in forensic autopsies from Turkey declared as natural sudden cardiac death following complete autopsy and toxicologic analysis. There was a male preponderance (M/F=4), with a mean age of 50.     

Homicide in Adana, Turkey: a 5-year review

Violence is a significant public health problem. Thus, so as to prevent this problem, homicide, the severest form of violence depriving a human being of his right to live, deserves a detailed examination. This study is a retrospective research examining the 2951 cases of medicolegal autopsies in Adana during a period of 5 years (1997-2001). Among these cases, 620, which were determined to be homicidal, were taken into the scope of this study. The cases were examined with respect to sex, age groups, the method used during the act of homicide, the number and the localization of the wounds on the body. A total of 620 (21%) of the medicolegal autopsies conducted within this period were homicides. Of these cases, 515 (83.06%) were male and 105 (16.94%) female, and the rate of the males to females was 4.9; 72.74% of the victims were between the ages of 21 and 50. It was seen that 54.83% of the homicides involved firearms, while 35.16% of the victims were stabbed to death with a cutting object. It was also determined that the victims suffered a single wound in 47.35% of firearm-related murders and 29.35% of stabbings resulted in death. Alcohol was found in the blood of 7.58% of the homicide victims, while none had any illicit drugs.     

Childhood foreign body aspiration in Istanbul, Turkey

In this study, we present the data concerning 10 children (six boys, four girls) with a diagnosis of foreign body aspiration (FBA) amongst 19,951 cases that underwent autopsy between the years 1996-2002. Eight of the children were under 2 years old. All the incidents took place at home. One of the parents was in the company of the child at the time of incident. At least one of the risk factors was positive. Interestingly, all of the aspirated material was related to food except one. Only four cases had subpleural petechiae, whereas five of the seven subjects undergoing histopathological evaluation had edema, hyperemia or intra-alveolar fresh bleeding. The legal instructions, which regulate the standards of toy materials, came into force at the end of 2003. Even though the legal instructions regulating the size and consistency of toys are very important to prevent FBA, we believe that the education of the parents and carers in the prevention of food aspiration is of greater importance.     

Suicides by jumping from Bosphorus Bridge in Istanbul

Suicide methods may differ widely. One of these methods is jumping from a height. The people who choose a high bridge for jumping may be considered as a special group. In this study, we evaluated the demographic data, mortality rates and postmortem findings of individuals who jumped from the 64m high Bosphorus Bridge of Istanbul. For this purpose, we reviewed registration forms of two police centers on both sides of the bridge, court archives, and postmortem examination reports of the deceased for a period of 10 years between 1986 and 1995.During this period, 65 people had jumped from the Bosphorus Bridge of Istanbul. The mortality rate was 96.9%, as two individuals survived their suicide attempt. The male/female ratio for attempts was 15:1 (61 males, four females) in this study in contrast to the male/female ratio of 1.6:1 for all suicidal cases in Turkey. The average age of subjects was 29.2 (median: 26, range: 16-54). The frequency was highest in the 15-24 age group with a rate of 38.4%. People who jumped from the bridge were younger than other reported cases of suicide. For all methods of suicide in Turkey 53.5% of subjects are under the age of 35. However, in our study, 75.8% of individuals who committed suicide from the Bosphorus Bridge were under the age of 35. Unmarried people accounted for 56% of the individuals whose marital status was known. 20.8% of the individuals whose employment status was known were unemployed. 59% of the cases whose education was known were high school graduates. The highest rate of suicide by jumping was in 1990, and occurred during the fall and winter months, primarily between the hours of 24.00 and 06.00. The Bosphorus Bridge is forbidden to pedestrians. Arrival method to the bridge was by a taxicab in 53.3% of the cases. 91% of the recovered bodies had been discovered during the first 2 days of jumping. Positions on impact with the water were estimated according to external and internal injuries. Among the cases in which the position of impact could be assessed by external and internal injury patterns, combined lateral and horizontal positions were most common.