Results of the GEP-ISFG collaborative study on two Y-STRs tetraplexes: GEPY I (DYS461, GATA C4, DYS437 and DYS438) and GEPY II (DYS460, GATA A10, GATA H4 and DYS439)

A collaborative exercise was carried out by the Spanish and Portuguese ISFG Working Group (GEP-ISFG) in order to evaluate the performance of two Y-chromosome STR PCR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The participating laboratories were asked to type three samples for the eight markers, using a specific amplification protocol. In addition, two control samples, with known haplotypes, were provided. The results obtained by the 13 different participating laboratories were identical, except for two laboratories that failed to type correctly the same two samples for GATA C4. By sequence analyses, two different GATA C4 allele structures were found. One control sample (allele 21) and two questioned samples (allele 22, correctly typed by all the laboratories, and allele 25) presented the following repeat structure: (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)n, but different from the one found for allele 26 in one sample included in this exercise, as well as in the second control sample (allele 23), namely (TCTA)4(TGTA)2(TCTA)2(TGTA)2(TCTA)2(TGTA)2(TCTA)n. The collaborative exercise results proved that both Y-tetraplexes produce good amplification results, with the advantage of being efficiently typed using different separation and detection methodologies. However, since GATA C4 repeat presents a complex structure, with alleles differing in sequence structure, efficient denaturing conditions should be followed in order to avoid typing errors due to sizing problems.     

Effects of pretrial juror bias,strength of evidence and deliberation process on juror decisions: new validity evidence of the Juror Bias Scale scores

This study examines the validity of the Juror Bias Scale scores in relation to the mock juror decisions reached in two real life homicide cases before and after the deliberation process. The judicial cases used varied in terms of the ambiguity of the evidence presented at both trials. The WLS methodology for statistical modelling of categorical data was used to analyse data. The findings indicated that the Juror Bias Scale scores successfully predict the verdicts and other related questions before and after deliberations in the case with ambiguous evidence. Furthermore, deliberations caused a generalisation effect on the pretrial juror bias in such a case, and enhanced the differences between defense-biased and prosecution-biased jurors in the verdicts delivered after deliberations. The implications of these findings are discussed in relation to the use of pretrial juror bias questionnaires in jury selection.     

Natural Disasters,Human Development and Poverty at the Municipal Level in Mexico

This article analyses the effects of natural disasters on human development and poverty levels at the municipal level in Mexico. Using several sources, we build a panel of data in order to uncover if different natural shocks can affect social indicators. After controlling for geographic and natural characteristics which can make municipalities more hazard prone, as well as for other institutional, socio-economic and demographic pre-shock characteristics, in addition to using fixed effects, we find that general shocks, especially from floods and droughts, lead to significant drops in both types of indicator.     

Some individual differences in perception of the evidence and the verdict choice

Abstract

For the last century, social research has provided evidence that contradicts the idea of objectivity in judicial procedure. As a result, research on jurors' potential bias has emerged. We propose an alternative to traditional jury, one in which social researchers collaborate with judges and lawyers, providing them with information on the juror bias. Legal authoritarianism is one of the characteristics of personality, which seems to be linked to juror verdicts. We present two studies, which develop a specific measure for this variable. The first study shows the relevance of legal authoritarianism as a variable to describe the psychological profile of juror-eligible respondents. The second study explores the relationship between legal authoritarianism and interpretation of the evidence, as well as the potential of this variable to predict verdicts.     

Results of the GEP-ISFG collaborative study on the Y chromosome STRs GATA A10, GATA C4, GATA H4, DYS437, DYS438, DYS439, DYS460 and DYS461: population data

The Spanish and Portuguese ISFG Working Group (GEP-ISFG) carried out a collaborative exercise in order to asses the performance of two Y chromosome STR tetraplexes, which include the loci DYS461, GATA C4, DYS437 and DYS438 (GEPY I), and DYS460, GATA A10, GATA H4 and DYS439 (GEPY II). The groups that reported correct results in all the systems were also asked to analyse a population sample in order to evaluate the informative content of these STRs in different populations. A total of 1020 males out of 13 population samples from Argentina, Brazil, Costa Rica, Macao, Mozambique, Portugal and Spain were analysed for all the loci included in the present study. Haplotype and allele frequencies of these eight Y-STRs were estimated in all samples. The lowest haplotype diversity was found in the Lara (Argentina) population (95.44%) and the highest (99.90%) in Macao (China). Pairwise haplotype analysis showed the relative homogeneity of the Iberian origin samples, in accordance with what was previously found in the European populations for other Y-STR haplotypes (http://www.ystr.org). As expected, the four non-Caucasian samples, Macao (Chinese), Mozambique (Africans), Costa Rica (Africans) and Argentina (Lara, Amerindians), show highly significant Phist values in the pairwise comparisons with all the Caucasian samples.     

Characterisation of 12 new alleles in the STR system D18S51

D18S51 alleles 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38 and 40 were detected and sequenced; these new alleles are mistyped with commercial STR kits, causing presumptive null alleles or false exclusions when paternity testing.     

Latex agglutination for bacterial antigens and meningococcus PCR: two useful tools in legal sudden deaths

Bacterial infections are considered to be a major cause of sudden deaths. The recognition of infections caused by Neisseria meningitidis is an essential duty of medicolegal offices due to the risk of secondary cases. Since other microorganisms, such as Haemophilus influenzae and Streptococcus pneumoniae, are also involved in infectious sudden deaths, the identification of the pathogen responsible for death is essential in order to establish a positive diagnosis while also preventing secondary meningococcal cases. However, because of the unreliability of culture methods used for autopsy specimens and the fragile nature of the microorganisms, other techniques were used. In this study, the detection of specific antigens of N. meningitidis (serogroups A, B, C, Y and W135), H. influenzae type b, S. pneumoniae and Group B Streptococcus was undertaken in 40 samples from sudden death cases in legal procedures with a latex agglutination test. In addition, a meningococcus polymerase chain reaction (PCR) assay (ctrA, crgA and siaD genes) was also used as a corroboration method for positive N. meningitidis agglutinations. Eleven cases of sudden death were confirmed to be due to meningococcus while one case was confirmed to have been caused by H. influenzae type b fulminant epiglottitis. Rapid laboratory diagnosis of meningococcal infection allowed contacts management and notification to the health authorities. From the point of view of the authors, forensic diagnosis of unascertained deaths should include latex agglutination and meningococcus PCR when a fulminant infection by N. meningitidis or H. influenzae is suspected as well as in deaths where the cause is unclear.