Detection of P1 antigen in blood stains by absorption elution test with protease C

Some lots of goat anti-P1 sera intended for analysis of liquid blood can detect the antigen in blood stains by the absorption-elution test.     

The diagnosis of an ABO(H) ABSe group in the traces of saliva, sperm and vaginal secretion using mixed agglutination reaction

Experimental spots of the saliva, sperm, vaginal secretion from persons with groups Ase, Bse, ABSe and Abse were studied with mixed agglutination reaction (MAR) using hemagglutinating sera anti-A and anti-B (heteroimmune, isoimmune), monoclonal antibodies. MAR with monoclonal antibodies was able to diagnose ABSe group not only in the spots of saliva, but also in the spots of sperm and vaginal secretion where heteroimmune sera failed.     

Diagnostics of the AB group of AB0(H) system in saliva traces

On the basis of the mixed agglutination reaction of the saliva spots, obtained from persons with groups A, B, and AB (extractors) as well as mixtures of saliva obtained from the above subjects in various combinations, were experimentally studied. It was shown to be possible to diagnose the AB group both in the "pure" saliva traces and as an admixture of the AB group saliva to the groups A and B saliva obtained by using the anti-A and anti-B hetero-immune gemagglutinating sera. Our results confirm the previously published opinion on that antigens A and B of the ABse group saliva are positioned in the cis-acting mode, i.e. they are located on one molecule of surface structure of the epithelial (buccal) cell.