Typeability of AmpFlSTR SGM Plus loci in brain and thyroid gland tissue samples incubated in different environments

Autolysis and putrefaction are crucial factors responsible for degradation of cells, tissues, and organs. Postmortem changes may assume different course depending on extrinsic and intrinsic conditions. The aim of the study was assessment of environmental effect on typeability of AmpFlSTR SGM Plus loci: D3S1358, VWA, D16S539, D2S1338, D81179, D21S11, D18S51, D19S433, TH01, FGA, and gender marker amelogenin. Brain and thyroid gland tissue specimens collected during autopsies of five persons aged 20-30 years were incubated at 21 degrees C and 4 degrees C in different environmental conditions. DNA was extracted by organic method from tissue samples collected in 7-day intervals and subsequently typed using AmpFlSTR SGM Plus kit and ABI 310. A fast decrease in typeability rate was seen in specimens incubated in peat soil and in sand. Brain tissue samples were typeable in all AmpFlSTR SGM Plus loci within 126 days of incubation at 4 degrees C. Faster DNA degradation was recorded in thyroid gland specimens. In samples with negative genotyping results, no DNA was found by fluorometric quantitiation.     

STR data for the AmpFlSTR SGM Plus loci from Warmia and Mazury (NE Poland)

Allele frequencies for the ten STRs included in the AmpFlSTR SGM Plus kit were determined in a sample of 320 unrelated individuals born in the region of Warmia and Mazury (NE Poland). All loci met Hardy-Weinberg expectations. Exact tests disequilibrium analysis revealed only one departure from independence out of 45 pair-wise comparisons of the 10 loci.     

Y-chromosome STR haplotypes and alleles in the ethnic group of Polish Tatars residing in the Northeastern Poland

Haplotype and allele frequencies for eight Y-STRs were determined in a population sample of the ethnic group of Polish Tatars residing in the Northeastern Poland. A total of 125 unrelated males produced 101 different haplotypes. Thirteen haplotypes occurred more than once, while 88 haplotypes were unique. The observed gene diversity was 0.9638. The ethnic group of Polish Tatars can be distinguished from the autochthonous Poles based on AMOVA estimates in all the analyzed systems except DYS385.     

Genetic data on 15 STRs in a population sample of religious minority of Old Believers residing in the northeastern Poland

This study provides a 15 STRs database for a population sample of Old Believers (n = 136) living in the northeastern Poland for the use as a highly discriminatory system of genetic markers in population studies and in personal identification. Significant differences revealed between the Old Believers and the autochthonous Poles by using RxC test as well as F(ST) and F(IS) estimates suggest a certain degree of genetic isolation of this religious minority.     

Population genetics of Y-chromosome STRs in a population sample of the Lithuanian minority residing in the northeastern Poland

Haplotype and allele frequencies for the 12 Y-STRs were determined in a population sample of 124 unrelated males--members of the Lithuanian minority residing in the northeastern Poland. Three of the haplotypes were encountered in duplicate, while 118 haplotypes were unique. The overall gene diversity was 0.9952. Analysis of molecular variance revealed that the Lithuanian minority can be distinguished from the autochthonous Poles, although these two populations are very close to each other. This database study provides an essential precondition for applying Y-chromosomal STRs estimates in forensic identification of male DNA and tracing of paternal lineages.     

Y-chromosome STR haplotypes in a population sample of the Byelorussian minority living in the northeastern Poland

Haplotype and allele frequencies for the eight Y-STRs were determined in a population sample of 156 unrelated Byelorussian males. The most common haplotype was shared by 3.85% of the sample, while 121 haplotypes were unique. The gene diversity was 0.98.     

Allele distribution of 15 STR loci in a population sample of Byelorussian minority residing in the northeastern Poland

Allele frequencies for the 15 STRs included in the AmpFlSTR Identifiler kit were determined in a population sample of 212 unrelated individuals of the Byelorussian minority residing in the northeastern Poland. All loci, except TPOX, met Hardy-Weinberg expectations. Exact tests disequilibrium analysis revealed 5 departures from independence out of 105 pairwise comparisons.     

Life Satisfaction in Latin America: A Size-of-Place Analysis

In Latin America, countries can be divided into two groups: those where people in villages and small towns are happier than people in middle sized cities, and those where they are less happy. Four possible reasons for these patterns are explored: level of economic development, social values, public social spending, and presence of indigenous population. In regressions on happiness of each explanatory factor separately, development and public social spending (especially on health, housing, and social security) are the most important. In a joint regression, public social spending is found to be the main explanation of the different happiness patterns.