STR analysis of artificially degraded DNA-results of a collaborative European exercise

Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak height and balance as well as the occurrence of artefacts. A number of common problems were identified based on these results such as strong peak imbalance in heterozygous genotypes for the larger short tandem repeat (STR) fragments after increased PCR cycle numbers, artefact signals and allelic drop-out. Based on the observations, strategies are discussed to overcome these problems. The strategies include careful balancing of the amount of template DNA and the PCR cycle numbers, the reaction volume and the amount of Taq polymerase. Furthermore, a careful evaluation of the results of the fragment analysis and of automated allele calling is necessary to identify the correct alleles and avoid artefacts.     

Parties, rules and government legislative control in Central Europe: The case of Poland

A distinguishing feature of Central European polities is a strong policy-shaping role of parliaments. This article demonstrates how party political and procedural factors set the scene for the executive's loss of legislative control in Poland. Parties undermine the governmental grip because of their limited internal cohesion and competitive coalitional strategies. Parliamentary rules reinforce such party effects. The executive can shield its bills from amendments by relying mainly on partisan controls, not formal privileges. But, as an analysis of over 300 bills shows, when party discipline and coalition cooperation are in short supply, partisan controls are ineffective as instruments of legislative control.     

Genetic data on 10 STR loci a population of western Poland

Allele frequencies for 10 STRs included in the AmpFlSTR SGM Plus kit were determined in a population sample of 668 unrelated individuals living in western Poland. All loci met Hardy-Weinberg expectations. Exact tests disequilibrium analysis revealed two departures from independence out of 45 pairwise comparisons. The combined matching probability (MP) and power of exclusion (PE) for all 15 loci are 2.56 x 10(-13) and 0.99996, respectively.     

Usefulness of two methods of isoelectric focusing for the erythrocyte acid phosphatase phenotypes determination in human bloodstains

The authors tried to compare the usefulness of the isoelectric focusing of EAP in bloodstains on 0.2 mm polyacrylamide gel with their method of determination of the enzyme on 1 mm polyacrylamide gel. Both methods turned out to be useful but better results were obtained on 0.2 mm gel. Isoelectric focusing on the ultra-thin gel is more sensitive; it gives clear enzyme strips, takes less time (30 min) and demands about half the amount of material.     

Property in Body Parts and Products of the Human Body

An intriguing question, which until recently had not been directly explored by the courts, is the extent to which English law recognises body parts and products of the human body as property capable of ownership. Although the common law currently recognises no general property in a dead body (and only limited possessory rights in respect of it), this apparent “no-property rule” provides no justification, it is submitted, for denying proprietary status to parts or products of a living human body. The recent decision of the Court of Appeal in Yearworth v. North Bristol NHS Trust ([2009] EWCA Civ 37) lends strong support to the view that genetic material (as the product of a living human body) is capable of ownership, at least in the context of a claim in the tort of negligence and bailment. This article examines the various issues by reference to both English and Commonwealth authority.

Improving public services by mining citizen feedback: An application of natural language processing

Research on user satisfaction has increased substantially in recent years. To date, most studies have tested the significance of predefined factors thought to influence user satisfaction, with no scalable means of verifying the validity of their assumptions. Digital technology has created new methods of collecting user feedback where service users post comments. As topic models can analyse large volumes of feedback, they have been proposed as a feasible approach to aggregating user opinions. This novel approach has been applied to process reviews of primary care practices in England. Findings from an analysis of more than 200,000 reviews show that the quality of interactions with staff and bureaucratic exigencies are the key drivers of user satisfaction. In addition, patient satisfaction is strongly influenced by factors that are not measured by state-of-the-art patient surveys. These results highlight the potential benefits of text mining and machine learning for public administration.     

Plurality support for democratic decay: the 2019 Polish parliamentary election

Abstract

The 2019 parliamentary election in Poland resulted in continuity of the incumbent PiS party-coalition. The election saw, for Poland, an unprecedented turnout of 62%. The 44% support for PiS translated into a 51% majority in the Lower House. The ability of the three opposition blocs (KO, SLD and PSL) to coordinate their political campaign for the upper chamber resulted in their victory – PiS lost the majority in the Senate. This post-2019 period thus starts as parliamentary cohabitation. The preceding four years had witnessed repeated violations of the constitution by the government (including attempts to dismantle the separation of powers and to turn public media into a partisan propaganda machine) accompanied by a general anti-liberal and anti-European stance. The electorates of the two major party-blocks have polarized in terms of their socio-demographic features. The new government does not differ much from the pre-election one, with the same PM and most ministers. The first weeks of its governing indicate that the government’s general as well as sectoral policies will be continued, including the controversial, illiberal ones. Finally, the election though free, was unfair, if for no other reason than the simple one of the partisan nature of the public media.