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The Application of Flow Cytometry as a Rapid and Sensitive Screening Method to Detect Contamination of Vitreous Humor Samples and Avoid Miscalculation of the Postmortem Interval 下载免费PDF全文
Cristina Cordeiro M.Sc. Rafael Seoane Ph.D. Ana Camba M.Sc. Elena Lendoiro M.Sc. María S. Rodríguez‐Calvo Ph.D. Duarte N. Vieira Ph.D. José I. Muñoz‐Barús Ph.D. 《Journal of forensic sciences》2015,60(5):1346-1349
Research into maximizing the speed, precision, and reliability of estimating the postmortem interval (PMI) has been a recurring object of investigation and methodologies based on the vitreous humor (VH) have provided good results. However, contamination from causes not readily apparent, such as blood, can occur, and thus lead not only to an erroneous estimation of PMI, but also interfere with the correct identification of other substances in the VH. We have developed a flow cytometry method which quantifies blood contamination and is able to detect erythrocytes in 1:750,000 dilution of contaminated VH which affects the results of hypoxanthine. It is an improvement on the previous more complex mass spectrometry method, being faster, more sensitive, and readily available. As such, it could be proposed for the rapid screening of appropriate samples by detecting and eliminating blood contaminated samples from PMI estimation. 相似文献
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Martínez B Caraballo L Barón F Gusmão L Amorim A Carracedo A 《Forensic science international》2006,160(2-3):221-223
Allele frequencies, together with some parameters of forensic interest were estimated for nine STRs included in the AmpF/STR Profiler kit (CSF1PO, D3S1358, D5S818, D7S820, D13S317, FGA, TH01, TPOX and vWA) in a sample of 215 unrelated individuals from Cartagena (Colombia). For all loci, no significant deviations from Hardy-Weinberg equilibrium were observed. Comparative analysis results between our data and those from other Colombian and African population samples revealed significant differences, except with two Colombian Caribbean Coast sub-regions. 相似文献
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Research evaluation is increasingly influenced by quantitative data. We focus on the influential Web of Science Journal Citation Reports (JCR) ranking of law journals and critically assess its methodology. In particular, we consider the existence and impact of a tacit citation cartel between US law reviews. A study of 45 US student‐edited (SE) and 45 peer‐reviewed (PR) journals included in the category of Law in the JCR revealed that PR and SE journals are more inclined to cite members of their own class and that this phenomenon is more pronounced in SE generalist journals, reflecting tacit cartelistic behavior generated by deeply entrenched institutional practices. Because US SE journals produce more citations than PR journals, the fact that their citations are directed almost exclusively to SE journals elevates their scores and distorts the journals' ranking and can consequently undermine the flow and creation of ideas. We discuss policy measures that can counter the adverse effects of this situation. 相似文献
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Uri Bar‐Noi 《Diplomacy & Statecraft》2013,24(3):110-133
New evidence from the Russian archives offers proof that Winston Churchill's calls for top‐level talks, 1953–54, were greeted with mistrust and scepticism by Moscow. Soviet policy makers realized that Churchill was weak, entirely dependent on American policy and unable to act as mediator between East and West. They were also sceptical as to whether any real progress could be made during an Anglo‐Soviet summit meeting and, instead, pressed for four‐power talks at Foreign Minister level. Finally, the Soviet records shed light on the impact of the power struggle within the Kremlin on its responses to Churchill's initiative, with Vyacheslav Molotov preventing Georgi Malenkov from meeting the British Prime Minister. 相似文献
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Facial reconstruction is the approximation of an antemortem face from human skeletal remains. Since the nineteenth century, several methods have been developed for reconstruction of the face; all of them require the measurement of average tissue thicknesses at various points on the face. To our knowledge, there are no publications on soft tissue thickness in the Turkish population. In addition, there are few publications on the value of magnetic resonance imaging (MRI) in measuring soft tissue thickness for forensic sciences. The aim of this study was to create a reference database of facial tissue thickness in the Turkish population, and to present data illustrating the successful use of MRI for this purpose. The study included 161 patients (79 males and 82 females) between the ages of 18 and 78 who had undergone brain MRI in our radiology clinic, and showed no sign of maxillofacial pathology. Measurements were taken at 9 points at the midline; glabella, nasion, end of nasals, mid-philtrum, upper lip margin, lower lip margin, chin-lip fold, mental eminence, and beneath chin points. The mean values for these points in the patient sample population were determined, and differences related to age, sex, and body mass index (BMI) were calculated. The values were then compared to the findings of the Manhein study. 相似文献
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Jose M. Suárez‐Peñaranda M.D. Ph.D. Cristina Cordeiro M.D. Marisol Rodríguez‐Calvo M.D. Ph.D. Duarte N. Vieira M.D. Ph.D. Jose Ignacio Muñoz‐Barús M.D. Ph.D. 《Journal of forensic sciences》2013,58(6):1644-1647
The role of cardiac inhibitory reflex as a potential cause of death is still a matter of debate. This study reports two cases of death under unusual circumstances. Case 1 corresponds to a man found hanging where the role of ligature compression of the carotid sinus became relevant as a possible explanation of death. In Case 2, the participation of a vasovagal syncope was clearly triggered by the laryngoscopic procedure. It is proposed that cardiac inhibitory reflex should be taken into account in those cases of unexpected death, which fulfills the following three criteria: (i) The investigation of the circumstances of the death is consistent with a hypothesis of cardiac arrest. (ii) A typical triggering peripheral stimulus is present. (iii) The performance of a complete autopsy cannot rule out the participation of a cardiac inhibitory reflex in the cause of death. 相似文献