Enhanced kinship analysis and STR-based DNA typing for human identification in mass fatality incidents: the Swissair flight 111 disaster

A bioinformatic tool was developed to assist with the victim identification initiative that followed the Swissair Flight 111 disaster. Making use of short tandem repeat (STR) DNA typing data generated with AmpFlSTR Profiler Plus (PP) and AmpFlSTR COfiler(CO) kits, the software systematically compared each available STR genotype with every other genotype. The matching algorithm was based on the search for: (i) direct matches to genotypes derived from personal effects; and (ii) potential kinship associations between victims and next-of-kin, as measured by allele sharing at individual loci. The software greatly assisted parentage analysis by enabling kinship evaluation in situations where complete parentage trios were unavailable and, in some situations, with distantly related relatives. Exclusion of fortuitous kinship associations (FKA) was made possible through the recovery at the disaster site of at least one remains for every sought-after victim, and was incorporated into the kinship software. The data from the 13 combined STR loci produced 6 and 23 times fewer FKAs when compared with PP alone and AmpFlSTR Profiler (PR) alone, respectively. Identification leads or confirmations of identification were obtained for 218 victims for which DNA reference samples (personal effects and kin) had been submitted. Confirmation of an inferred kinship association was sought through frequency and likelihood calculations, as well as corroborative data from other identification modalities. The use of a simple, yet powerful, automated genotype comparison approach and the use of megaplexes with high power of discrimination (PD) values extended considerably the identification capabilities in the case of the Swissair disaster. The DNA typing identification modality proved to be a valuable component of the large arsenal of identification tools deployed in the aftermath of this disaster.     

Systematic analysis of stutter percentages and allele peak height and peak area ratios at heterozygous STR loci for forensic casework and database samples

To assist the interpretation of STR DNA typing results from forensic casework samples containing mixtures, the range of heterozygous allele peak height and peak area ratios (HR) and stutter percentages (stutter %) for the loci comprised in the AmpFlSTR Profiler Plus (PP) kit were assessed on 468 database and 275 casework single source samples. Stutter % medians were similar for database and casework samples, ranging from 2% to 7%. The upper limit of the stutter value range was 16%, calculated as median +3 SD, although lower locus-specific values could be used. HR medians were 93 +/- 6.5% for database samples, 88 +/- 12% for casework samples. For casework samples, the maximum signal imbalance noted was 52%, calculated as median -3 SD. No significant difference was observed between peak height and peak area calculated values. This study shows the importance of selecting the proper reference database for the establishment of HR threshold values.     

Flies eggs: a new method for the estimation of short-term post-mortem interval?

Three modeling methods were used to estimate the time for laying of Lucilia sericata eggs (Diptera, Calliphoridae) after measurements of hatching times at several constant temperatures. These models were tested first under controlled conditions with 19 profiles of fluctuating temperatures, and on the other hand under field conditions on mice. All three models were allowed to determine the time of laying within a period of about 2h, and consequently this determines the time of death, as this species lays immediately after death under favorable conditions. Rearing of eggs sampled on a corpse may therefore contribute to the determination of a short post-mortem interval (PMI).     

STR DNA typing: increased sensitivity and efficient sample consumption using reduced PCR reaction volumes

Improvements in detection limits/sensitivity and lower sample consumption are potential benefits of reducing PCR reaction volumes used in forensic DNA typing of crime scene samples. This premise was studied first with experimental mixtures and a nine-loci megaplex, which demonstrated stochiometric amplification and accurate detection. Next, adjudicated casework samples were subjected to amplification under 15 different template DNA to PCR reaction volume ratios. Reduction of PCR reaction volume and DNA down to 10 microL and 0.500 ng, respectively, produced identical profiles with the same signal intensity and heterozygous allele peak height ratio (HR). Reduction to 5 microL and 0.063 ng yielded HR values that were slightly affected in one to three STR loci. PCR reaction volume reduction can enhance detection and sensitivity while reducing the consumption of irreplaceable crime scene samples.     

Emerging Indicators and Bureaucracy: From the Iron Cage to the Metric Cage

ABSTRACT

Among the New Public Management (NPM) tools that have been implemented, one of the most common is use of indicators. Many studies have argued that imposition of an indicator entails a bureaucratization process. But the scholarly debate on these instruments has been shaped by a vertical perspective and has been focused on the effects of top-down indicators on organizations at the local level. In this paper, we wish to lay the emphasis on an emerging indicator, constructed and used by the actors themselves within a local forest management unit. We wish to draw attention to the manner in which, at the micro-level, a performance measure is worked out. We argue that these emerging indicators place a new locus of power in the hands of rangers but, rather than emphasizing hierarchy and chain of command, such indicators do not contribute to development of a Weberian iron cage. In contrast, there appears a metric cage, a mix of pragmatic rationality (instrumental and communicative), non-fixed rules, and technical-normative authority.     

Qualitative Comparative Analysis (QCA), Anno 2013: Reframing The Comparative Method's Seminal Statements

This review article examines the ways in which QCA is being (re)framed by some main authors in the field, in a context of expansion and diversification of this approach and set of techniques. Charles Ragin's seminal The Comparative Method (1987) is first synthetized in the form of eight statements which are then confronted to eight recent book‐length publications: three QCA textbooks and five methodological volumes also touching upon QCA. On the whole, it appears that most statements have been considerably refined, both conceptually and technically, whereas only one statement is not taken on board anymore. In addition, QCA is being reframed and extended in different ways beyond Charles Ragin's initial statements.     

Political fragmentation, party ideology and public expenditures

In this paper we propose an original model of competition for effective political power between majority and opposition coalitions. The model indicates that the electoral margin of the majority and the fragmentation of both coalitions are key variables that determine their effective political power. We estimate the model in the case of the French départements. Our econometric results support the model and show that the per capita social expenditures in the French départements depend on the effective political power of the majority.     

Procéduralisation et indécidabilité

Sans résumé     

The Knowledge-Based Economy: Conceptual Framework or Buzzword?

In the last two decades, concepts have appeared that have influenced and even defined entire science and technology policies in Western countries: high technology, national system of innovation, information economy, knowledge-based economy, and the new economy. In all these policy developments, the OECD, acting as a think tank for its member countries, has been an important promoter of these concepts, turning them into buzzwords. This article looks at the concept of knowledge-based economy in order to explore the crucial role of the OECD in its dissemination and, above all, the role statistics have played in shaping policy discourses.