Primitiveness and the flight from modernity: sociology and the avant-garde in inter-war France

This paper examines the links between the French school of sociology and anthropology and the surrealist avant-garde in inter-war Paris by unearthing their common reliance on a cross-cultural critique of European modernity. Accordingly, the paper focuses on how both currents – and notably the Collège de sociologie – used representations of a mythical ‘primitive’ condition to produce an outside of modern society and an alternative socio-cultural universe from which Europe's pathologies could be diagnosed. This self-critique through a comparative lens aimed to radically situate, relativize and decentre the phenomena of rationalization, disenchantment and anomie characterizing modernity, which could be shown to be neither natural, eternal, universal nor inevitable, but rather the socio-historical products of developments in a particular culture during a specific period of its history.     

Fast PCR amplification using AmpFlSTR Identifiler: Second report

At ISFG2007, an earlier conference, we presented reports on two fast PCR cycling methods using AmpFlSTR Identifiler. In our current study, which involved PCR amplification using AmpliTaq Gold Fast PCR Master Mix, UP, and a standard PCR thermal cycler rather than the Fast PCR thermal cycler, we succeeded in allele typing while reducing PCR running times by half (to approximately 90 min).     

Case study of dissolving allele dropout

Allele typing performed using the original components of the AmpFlSTR Identifiler kit led to unbalanced peak heights and allele dropouts. However, allele typing was completely successful when substituting the QIAGEN Multiplex PCR Master Mix for the AmpFlSTR PCR Reaction Mix of the Identifiler kit. The successful range for allele typing was 250 pg to 1.5 ng of DNA. The running times for PCR amplification were reduced by about 30 min from the original protocol.     

Fast PCR amplification of AmpFlSTR Identifiler

To increase throughput for DNA typing, we examined fast PCR cycling using AmpFlSTR Identifiler by three methods. In this study, we reduced PCR running times by 1/3 to 2/3 (approximately 1-2 h). This means DNA typing, including PCR reactions, can be completed within a timeframe ranging from 90 min to 2 h and 30 min.