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Nondestructive Methods for Recovery of Biological Material from Human Teeth for DNA Extraction 下载免费PDF全文
Montserrat Hervella Ph.D. Maitane G. Iñiguez M.Sc. Neskuts Izagirre Ph.D. Alberto Anta Ph.D. Concepción de‐la‐Rúa Ph.D. 《Journal of forensic sciences》2015,60(1):136-141
The extraction of DNA from human skeletal remains applied to forensic, and evolutionary studies do not exclude risks, which are to be evaluated when working with unique specimens that could be damaged or even destroyed. In the present study were evaluated several nondestructive methods for recovering DNA instead of the most currently used pulverization method. Three different procedures to access inside the dental pieces (occlusal perforation, cervical perforation, and cervical cut) have been compared with the aim of recovering as many cell remains as possible to carry out a DNA extraction. Given the DNA quantitation results, a method was proposed that consists of a cervical cut to facilitate the access to the pulp cavity and a subsequent filing of the root canals down to the apex of the dental root. This methodology allows the recovery of both mitochondrial and nuclear DNA, with the minimum deterioration for the dental pieces. 相似文献
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After the constitution of the European Monetary Union (EMU), a new scope for monetary coordiantion is emerging in other regional areas. The standard approach to theoretically analyse the feasibility of a monetary union is the optimum currency areas (OCA) approach. Although this approach has been claimed to be non-operative, recent studies using synchronisation measures have shown a high potential applicability. This paper provides an empirical application of the OCA theory to a hypothetical East Asian monetary union centred on Japan’s currency. We find that despite the increasing synchronisation of macroeconomic determinants of a monetary area during 1980–2001, the perspective of an Asian currency union is still not economically feasible.
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Joan Costa-FontEmail: |
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Montserrat Guibernau 《The international spectator : a quarterly journal of the Istituto affari internazionali》2013,48(3):106-117
Various factors have triggered the recent shift from devolution to secession in Catalonia: the Aznar government’s lack of response to demands for greater autonomy for Catalonia, the legal challenging of the 2006 Statute of Autonomy of Catalonia and, increasingly, economic arguments as Catalan society endures a harsh economic crisis. After evaluating the impact of the Spanish transition to democracy upon younger generations’ expectations regarding the meaning and content of democracy in post-Franco Spain, it is argued that democracy based upon ‘consensus’ rather than ‘majoritarian democracy’ would be better suited to respond to national minorities’ demands in Spain. 相似文献
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In several respects, the EU represents both a novel system of quasi-supranational governance and a novel form of political community or polity. But it is also a fragile construction for it remains a community still in the making with an ambiguous sense of identity and within which powerful forces are at work. This paper has three main aims: first, to stress the shifting nature of Europe??s geographical frontiers and assess whether cultural frontiers have remained more stable throughout time. In particular, it examines the main criteria, which have traditionally been employed when having to decide who should be included and excluded from Europe. A different question concerns the requirements for EU membership and the monopoly of the adjective ??European?? by the EU, which somehow has become to be identified with Europe. Second, to explore the prospects for the emergence of a European identity. Here, I argue that European identity stands as a ??non-emotional identity?? in sharp contrast with traditional forms of national identity associated with intense nationalist feelings. Third, through the analysis of the most recent Eurobarometer (annual survey of EU??s public opinion) to examine the views of Europeans regarding the EU at a time of a major global economic crisis. To conclude, the paper explores the main challenges to be faced by a still incipient European identity. 相似文献
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Brion M Allegue C Santori M Gil R Blanco-Verea A Haas C Bartsch C Poster S Madea B Campuzano O Brugada R Carracedo A 《Forensic science international》2012,219(1-3):278-281
In developed countries, sudden infant death syndrome (SIDS) represents the most prevalent cause of death in children between 1 month and 1 year of age. SIDS is a diagnosis of exclusion, a negative autopsy which requires the absence of structural organ disease. Although investigators have confirmed that a significant percentage of SIDS cases are actually channelopathies, no data have been made available as to whether other sudden cardiac death-associated diseases, such as hypertrophic cardiomyopathy (HCM), could be responsible for some cases of SIDS. The presence of a genetic mutation in the sarcomeric protein usually affects the force of contraction of the myocyte, whose weakness is compensated with progressive hypertrophy and disarray. However, it is unclear whether in the most incipient forms, that is, first years of life, the lack of these phenotypes still confers a risk of arrhythmogenesis. The main goal of the present study is to wonder whether genetic defects in the sarcomeric proteins, previously associated with HCM, could be responsible for SIDS. We have analysed 286 SIDS cases for the most common genes implicated in HCM in adults. A total of 680 mutations localised in 16 genes were analysed by semi-automated matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDITOF-MS) using the Sequenom MassARRAY(?) System. Ten subjects with completely normal hearts showed mutated alleles at nine of the genetic variants analysed, and one additional novel mutation was detected by conventional sequencing. Therefore, a genetic mutation associated with HCM may cause sudden cardiac death in the absence of an identifiable phenotype. 相似文献
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Carles Pérez-Testor Josep A. Castillo Montserrat Davins Manel Salamero Marta San-Martino 《Journal of family violence》2007,22(2):73-80
Personality patterns, psychopathological symptomatology, dyadic relationship adjustment, and the characteristics of abuse
suffered by a sample of women who attended residential services for battered women in Barcelona (Spain) are evaluated in this
study. Over half the women had suffered psychological, physical, and sexual abuse on the part of her partner, mostly of long
duration, and this had also been suffered by their children. The results obtained using the MCMI-II show a relatively low
presence of anxious or depressive symptomatology and the predominance of a schizoid personality pattern. This means that clinical
efforts need to be directed towards countering emotional withdrawal and social isolation. The importance of studying interpersonal
patterns amongst battered women with a view to optimizing care and to preventing future victimization is also discussed. 相似文献
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Lucy C. Sorensen Montserrat Avila-Acosta John B. Engberg Shawn D. Bushway 《Journal of policy analysis and management》2023,42(4):941-970
U.S. public school students increasingly attend schools with sworn law enforcement officers present. Yet little is known about how these school resource officers (SROs) affect school environments or student outcomes. Our study uses a fuzzy regression discontinuity (RD) design with national school-level data from 2014 to 2018 to estimate the impacts of SRO placement. We construct this discontinuity based on the application scores for federal school-based policing grants of linked police agencies. We find that SROs effectively reduce some forms of violence in schools, but do not prevent gun-related incidents. We also find that SROs intensify the use of suspension, expulsion, police referral, and arrest of students. These increases in disciplinary and police actions are consistently largest for Black students, male students, and students with disabilities. 相似文献