1株抗须癣毛癣菌枯草芽孢菌的鉴定

为了探讨抗须癣毛癣菌感染新的治疗途径,从患须癣毛癣菌病兔自然恢复皮肤分离了1株细菌,经16S rDNA基因比对,并结合菌落形态进行了鉴定,通过体内外试验测定其对须癣毛癣菌的拮抗作用。结果显示,分离株能在营养琼脂培养基生长,菌落为皱褶圆形,菌体呈杆状,革兰染色阳性,基因序列分析与GenBank中的枯草芽孢杆菌相似率为99.8%,该分离株被鉴定为枯草芽孢杆菌。抗菌谱分析显示,分离株对须癣毛癣菌能形成抑菌圈,对白色念珠菌、平滑念珠菌、热带念珠菌、烟曲霉菌、黄曲霉菌没有抑制作用。该分离株与须癣毛癣菌混合培养,能明显抑制须癣毛癣菌生长。在皮肤涂抹试验中,该分离株能逆转由须癣毛癣菌引起的皮肤病变。本试验证实了分离的枯草芽孢杆菌对须癣毛癣菌有明显的拮抗作用。     

改善中小企业融资环境探讨

中小企业是社会生活中不可或缺的重要组成部分 ,但在其发展过程中遇到很多困难 ,其中融资困难是影响中小企业发展的主要问题。要解决融资难的问题 ,必须改革金融制度 ,拓宽融资渠道 ,完善融资体系 ,进而为中小企业创造良好的融资环境。     

Four Lessons for Developing Countries from the Trade Negotiations Over Access to Medicines

After the Agreement on the Trade-Related Aspects of Intellectual Property Rights (TRIPS) came into operation in 1995 developing countries have found themselves in a process of continual negotiation over intellectual property rights and access to medicines. These negotiations have taken place in the World Trade Organization and in the context of free trade agreements. The paper suggests that the only real win for developing countries has been the Doha Declaration on the TRIPS Agreement and Public Health in 2001. What have been the lessons for developing countries in a decade of negotiations over access to medicines? Drawing on themes of rule complexity and regulatory ritualism the paper discusses four key lessons for developing countries. It concludes by arguing that developing countries will do better if they adopt a networked governance approach to negotiation rather than continuing to rely on traditional coalition formation.     

猪生殖与呼吸综合征病毒GP5蛋白抗原表位基因的表达及其表达产物的抗原性

为了确定猪生殖与呼吸综合征病毒GP5蛋白A、B抗原表位之间的相互关系和对免疫的影响,应用PCR方法扩增出A、B抗原表位基因并分别克隆于原核和真核表达载体中。原核重组质粒表达的重组蛋白经Western-blotting检测证明,该重组蛋白具有良好的抗原性。将重组蛋白和真核表达质粒免疫小鼠,前者可诱导产生针对PRRSV的特异性ELISA抗体,但是此抗体没有中和活性;后者不能诱导免疫小鼠产生PRRSV特异性抗体。结果表明,中和抗体的产生不但与A、B表位的空间构象有很大的关系,如果蛋白质分子质量过小也不能诱导机体产生有效的免疫反应。     

“The Spectacle of Fearsome Acts”: Crime in the Melting P(l)ot in <Emphasis Type="Italic">Gangs of New York</Emphasis>

This paper explores the multi-layered representations of violence and crime in the recent Hollywood film Gangs of New York [Scorcese (2003) Miramax]. We use our exploration of this film to suggest that popular culture, even through its most mainstream products, can be seen as a critical criminological space where alternative views of law, crime and the state are made available. Rather than understanding Hollywood movies simply as vehicles for disseminating conventional mores, we suggest that they can furnish critical (and complex) points of view on law and crime and that the project of a critical criminology can be strengthened by engaging more forthrightly with these ubiquitous cultural forms.     

幼年牦牛松果体的光镜和电镜观察

利用光镜和电镜技术对幼年牦牛松果体的组织形态结构特征进行了研究。结果表明,光镜下,幼年牦牛松果体由松果体细胞、少量的神经胶质细胞、毛细血管和神经等组成。电镜下,松果体细胞的电子致密度低,细胞质内含有丰富的线粒体、粗面内质网、滑面内质网、高尔基复合体、微管、微丝和核糖体,典型异质细胞器突触带呈球形,多位于质膜附近。神经胶质细胞内的线粒体丰富,胞体突起呈球形膨大伸入到松果体细胞之间。松果体细胞以及神经胶质细胞间均存在突触和连接复合体。牦牛松果体内的毛细血管为连续型,远腹侧血管周围可见色素细胞。     

The challenge of institution-building in Asia and its implications for Asia-Europe relations

  East Asia, including Northeast Asia and Southeast Asia, has developed tightly-linked production/distribution networks through globalizing corporate activities. The vertical chain of production in East Asia has been even more sophisticated than economic integration in East Europe or Latin America. However, the political environment of East Asia for trade and investment has been far from borderless. The integration effort at the policy level has been very much limited so far, due to the historical background as well as geopolitics surrounding East Asia. The Asian currency/financial crisis provided these countries a historical turning point. After the burst of the crisis, East Asians realized that they have to take care of themselves in their difficulties, not depending on outside forces. A natural choice for them was to step into the realm of regionalism. In 1998, Japan and Korea officially announced that they would discard the long-lasting GATT/WTO-only approach and adapt the multi-layered approach, including both regionalism and multilateralism. The ultimate goal of regionalism would be a region-wide integration including ASEAN+3. As a steppingstone, Japan signed the Japan-Singapore Economic Partnership Agreement (JSEPA) in January 2002. In a parallel move, the ASEAN and China Leaders announced in November 2001 the establishment of an ASEAN-China Free Trade Area (ACFTA) within 10 years. This article will follow up the most recent advancement of regional institutional building in East Asia with the emphasis on peculiar characteristics of economic integration in the region and discuss its implications for Asia-Europe relations. This paper is heavily drawn from Kimura (2002, 2003).     

S&;T Parks and business incubators in middle-sized countries: the case of greece

This paper links the policy context regarding S&T parks to the objectives and characteristics of the established parks and incubators in a relatively small, converging European country. S&T parks and incubators in Greece during the past decade and a half have gradually shifted in terms of ownership and management from university/research institute to the private sector and in terms of financing from public to private funds, also involving the provision of seed capital and access to venture capital. This gradual shift toward more private engagement has created a variety of models out of which, it is hoped, successful innovative companies will emerge in larger numbers than in the past.

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