ABO位点限制性扩增片段长度多态性的研究

建立了PCR扩增、限制性酶切、8％（T）、5％（C）聚丙烯酸胺凝胶垂直电泳和银染检测ABO位点的限制性片段长度多态性的方法体系。应用Amp-RFLP技术对185名中国人（哈尔滨）ABO位点的基因频率和基因型分布进行了调查和统计分析。ABO位点特异片段长度为140～200bp，基因频率为0．2000～0．5568。6种基因型频率为0．973～0．3135，杂合度0．5838，Dp值0．7146。经H－W平衡吻合度检测，完全符合群体遗传多态分布。通过对11个家庭33名相关个体的分析，证明完全符合孟德尔遗传定律。ABO基因型检验适用于法庭科学的个体识别和亲权鉴定。     

Cheikh Anta Diop: The social sciences,humanities, physical and natural sciences and transdisciplinarity

ABSTRACT

This article presents a critical exposition of the contributions of Cheikh Anta Diop to a scientific understanding of ancient African history, race, and the study of culture. It sets out the history of Diop's successful struggle against flawed Eurocentric scholarship which sought to deny the contributions of ancient black Egyptians to world civilisation. Diop's intellectual odyssey across physics, linguistics, through anthropology, ethnology, genetics and history is recounted here to demonstrate the limitations of mono-, inter- and multidisciplinarity and clearly identifies him as a pioneer of transdisciplinarity in the field of knowledge production.