Exploring the Roots of Public Participation in the Controversy Over Embryonic Stem Cell Research and Cloning

In this study, analyzing nationally represented survey data collected in 2003, we consider the roots of issue-specific citizen participation in the controversy over embryonic stem cell research and therapeutic cloning. Building on past research, we pay particular theoretical attention to the role of issue engagements, the impact of church-based recruitment, and the influence of news media attentiveness. Given the increasing emphasis in science policy circles on creating new forms of public engagement, we also measure citizen willingness to attend and participate in a proposed local deliberative forum on the stem cell debate. Results indicate that traditional forms of citizen activism in the controversy over embryonic stem cell research and cloning is rooted almost exclusively in direct requests for participation through religious organizations rather than socio-economic differences among respondents, though issue engagement (measured as opinion intensity) and news attentiveness also play an important role. In terms of deliberative forums, traditional resource factors are significant, as the citizens who indicate they are most likely to participate in such a hypothetical local town meeting are generally highly educated, white, and younger. Above and beyond these resource factors, however, citizens willing to participate are also likely to have received requests to get involved in the debate at church, hold more intense feelings about the issue, and are paying closer attention to news coverage. In the future, in order to ensure the normative goals of diverse and/or representative participation, if actual deliberative forums are employed, these findings suggest that organizers will need to focus heavily on purposive sampling and turn out efforts.

The role of ASEAN institutes of strategic and international studies in developing security cooperation in the Asia‐pacific region

As the Association of Southeast Asian Nations (ASEAN) governments are facing new challenges, the need to re‐evaluate the significance of the track‐two activities has been recognised. As there has been insufficient research on the ASEAN Institutes of Strategic and International Studies (ISIS), this article analyses their role in the development of security cooperation. It shows that ASEAN‐ISIS has contributed to the establishment of the ASEAN Regional Forum (ARF) by analysing the common/cooperative security thinking, the establishment of an inter‐governmental forum for a security dialogue, and the extension of ASEAN's diplomatic style over a larger geographical area. As ASEAN‐ISIS has contributed to inter‐governmental cooperation by promoting ideas, this article concludes that the significance of their activities in contemporary Asian politics should be understood in terms of the introduction and promotion of such innovative ideas.     

鸡传染性支气管炎北京地方株S_1基因的克隆与鉴定

利用反转录套式聚合酶链反应（ＲＴｎｅｓｔｅｄＰＣＲ）技术从北京地区３株鸡传染性支气管炎病毒（ＩＢＶ）分离株（ＢＪ１,ＢＪ２,ＢＪ３）中扩增出１０５４ｂｐＳ１基因高变区。利用限制性内切酶ＳｉｎⅠ和ＰｓｔⅠ的酶切位点分析图谱进一步证实了ＲＴｎｅｓｔｅｄＰＣＲ的特异性。将３段Ｓ１基因分别克隆到ｐＵＣ１９质粒中,获得重组质粒ｐＵＣＩＢＶＳ１ＢＪ１,ｐＵＣＩＢＶＳ１ＢＪ２和ｐＵＣＩＢＶＳ１ＢＪ３。     

克隆人立法的宪法规制

克隆羊"多莉"诞生以来,克隆技术迅猛发展,克隆人问题的现实性日益彰显。我国秉持"禁止生殖性克隆、支持治疗性克隆"的指导思想,出台了相关规章禁止克隆人。然而,目前我国克隆人的相关立法有违背宪法上的法律保留原则和比例原则之嫌,存在着合宪性问题。建议全国人大根据法律保留和比例原则制定专门的《克隆技术管理法》,明确界定克隆人的相关概念,禁止任何人从事生殖性克隆,并明确规定监管机关的监管职责以及违法应当承担的法律责任,同时将生殖性克隆入罪、明确立法的"落日条款",以消解当前克隆人立法的合宪性问题,实现克隆人立法的宪法规制。     

番鸭细小病毒弱毒株VP2基因的分子克隆与序列测定

将番鸭细小病毒(MDPV)强毒Q株经番鸭胚连续传代,获得致弱株MDPV-26.根据已发表的MDPV的全基因序列,设计了1对引物LHMP7/LHMP8,同时在这2条引物中分别加入2种限制性核酸内切酶SacⅡ和KpnⅠ的酶切位点.应用PCR技术扩增了MDPV-26株的VP2基因片段.将扩增后的基因片段重组到pMD18-T质粒载体上,并对插入片段进行序列测定.测序结果表明,弱毒株MDPV-26与强毒株MDPV-Q的VP2基因序列相同率达99.7%,与国外分离株的同源性为98.3%,说明强、弱毒株的VP2基因序列变化不大.     

Biotechnology,Bioethics and Law: Europe's 21st Century Challenge*

Advances in biotechnology make possible many things which even a few years ago would have seemed unimaginable. However, the steady advance of biotechnological innovation raises difficult questions for ethicists and regulators. In the thirty‐fourth Chorley Lecture Noelle Lenoir analyses the European response to these challenges and calls upon European leaders to honour their commitment to human dignity and to give leadership in the emergent fields of embryo research, cloning and genetic enhancement.     

传染性腔上囊病病毒超强毒致弱株GZ20 VP2 基因的克隆和序列分析

根据已发表的52/70株传染性腔上囊病病毒(IBDV)基因组序列,设计并合成了一对特异扩增IBDV VP2基因的引物.以超强毒IBDV(vvIBDV)致弱株GZ20基因组为模板利用RT-PCR技术扩增出了1.5kb的cDNA产物,将VP2基因克隆于pUC119质粒上,得到重组pUC119质粒.并对VP2基因全序列测定,序列分析和聚类分析表明,该致弱株与无毒疫苗株PBG98和弱毒株CU-1非常相似,而与经典强毒株、超强毒株和变异株相差较大.这提示该致弱株属于标准血清Ⅰ型弱毒株.