堆型艾美球虫广东株3-1E基因的克隆与序列分析

根据GenBank中登录的堆型艾美球虫31E基因序列,设计了3条引物,以广东株堆型艾美球虫裂殖子总RNA为模板,利用反转录聚合酶链反应(RTPCR)扩增获得了31E基因部分片段,将这一片段克隆至pGEMTEasy载体中,经PCR、限制性内切酶分析和克隆片段的序列测定、比较,证实了克隆片段的可靠性。序列比较发现,所克隆的基因片段与Eimeriaacervulina美国株(US)、E.acervulinaQH株31EcDNA的核苷酸同源性分别为99.0%和99.2%,推导氨基酸的同源性分别为98.2%和97.6%。     

原位反转录PCR技术的应用

针对已有资料中对于以DNA为靶基因序列的原位 PCR介绍较多,而对于原位反转录PCR的介绍很少,但在实际研究工作中有很多检测的靶基因是 RNA(病毒的或基因组的 RNA)的实际情况,就原位反转录PCR技术的基本原理、影响试验结果的关键因素和步骤,如组织细胞的固定、引物和探针的选择、蛋白酶及DNA酶消化、假阳性及假阴性的产生等进行了概述,旨在通过探讨上述问题,对该技术的实际应用有所指导。     

口蹄疫病毒3D基因的真核表达及其表达产物的功能分析

利用RT-PCR技术扩增口蹄疫病毒(FMDV)编码RNA依赖的RNA聚合酶的3D基因,经测序确认后将其克隆到真核表达质粒载体pcDNA3.1( )中,重组质粒pcDNA3.1( )-3D经HindⅢ、XbaⅠ双酶切后转染至BHK-21细胞,用纯化的目的蛋白进行Western-blotting鉴定,并预测该3D基因表达产物的三级结构。结果显示,获得分子质量约55 ku的单一3D基因表达产物,其三级结构较为保守。利用RNA细胞内复制体系和荧光定量RT-PCR技术,证明3D基因表达产物在细胞内可促进口蹄疫病毒基因组的复制。     

Competitive Liberalization,Competitive Imperialism,and Intellectual Property

Aggressive pursuit of free trade agreements (FTAs) and customs unions (CUs) by major and minor trading powers alike challenges the conventional wisdom in favor of such pursuit – competitive liberalization. An equally plausible explanation for an active bilateral and regional trade agreement policy, one which effectively de-emphasizes multilateralism, may be competitive imperialism. The protection and enforcement of intellectual property rights is one area in which new provisions, going beyond multilateral rules, are being negotiated and written into FTAs and CUs. Such provisions may yield insights into which characterization of bilateralism and regionalism – competitive liberalization or competitive imperialism – is more apt. Rice Distinguished Professor, The University of Kansas, School of Law, Green Hall, 1535 West 15th Street, Lawrence, KS 66045-7577, USA. Tel. +1-785-8649224. Fax. +1-785-8645054. www.law.ku.edu. J.D., Harvard (1989); M.Sc., Oxford (1986); M.Sc., London School of Economics (1985); A.B., Duke (1984). Marshall Scholar (1984-86). Member, Council on Foreign Relations, Royal Society for Asian Affairs, and Fellowship of Catholic Scholars. Author, Modern GATT Law (Sweet & Maxwell 2005), International Trade Law: Theory and Practice (2nd ed. 2000, 3rd ed. forthcoming 2007-08), and Trade, Development, and Social Justice (Carolina Academic Press 2003). I am thankful to my Research Assistant, Mr. David R. Jackson (B.A., George Mason University, 1992; J.D. Class of 2007, University of Kansas), for his indispensable help on this work. I also am grateful to Dr. Mohammed El Said, University of Central Lancashire (UCLAN), for his consistent support and friendship, and for what he has taught and continues to teach me about international trade and intellectual property.     

建设高素质公安队伍要强化和完善三个机制

建设高素质的公安队伍 ,必须要有一个好的工作机制。这个工作机制就是强化和完善公开、平等的竞争机制 ,催人奋进的激励机制和全面严格的制约机制。     

公共服务的竞争性外包:比较丹麦和瑞典的政策与执行

本文比较和分析了丹麦和瑞典对公共服务实行竞争性外包的道路。人们通常认为 ,这两个国家不存在或者只有少量的外包现象。但事实正好相反 ,这两个国家都有很长的将公共服务外包出去的历史 ,而且自从 2 0世纪 80年代就开始争论和执行竞争性外包政策了。     

论高校图书馆核心竞争力的构建

高校图书馆要想在信息市场竞争中赢得主动,就必须构建自己的核心竞争力,而提高核心竞争力是通过建立特色图书馆、创新服务理念和管理机制、提高人员素质来实现的。     

竞争力优势理论与中小企业竞争战略

人力资本不足成为制约中小企业发展、创新和竞争优势形成的障碍,文章以迈克尔·波特(Michael Porter)的产业竞争模式分析理论框架为依据,指出技术创新是中小企业竞争力优势的基础,人才是中小企业发展的关键,提出了中小企业的竞争战略选择.     

鸡白细胞介素-2受体基因γ链的转录分析与表达

以脾单个核细胞总RNA为模板,对鸡白细胞介素-2受体基因γ链(chIL-2Rγ)进行了RT-PCR,获得了一1 047 bp的开放阅读框,编码由348个氨基酸残基组成的分子质量为37.8 ku的蛋白多肽。预测的鸡IL-2Rγ多肽链中包含4个保守半胱氨酸残基、1个WSXWS基序和7个N连接的糖基化位点。鸡IL-2Rγ与其他动物IL-2Rγ在氨基酸水平上的同源性仅为21.4%~38.2%。RT-PCR检测发现,鸡IL-2RγmRNA分布于大脑、小脑、脊髓、腔上囊、脾、胸腺、骨髓、盲肠扁桃体、腺胃、肌胃、空肠、卵黄囊憩室、回肠、盲肠、直肠、心脏、肾、肺、肝、骨骼肌和皮肤,而在十二指肠和睾丸中没有检测到其转录。构建了鸡IL-2Rγ胞外区的原核重组表达载体,进行了表达和鉴定。     

Subnational competitive authoritarianism and power-sharing in Bosnia and Herzegovina

ABSTRACT

Incremental democratic decline is evident in Bosnia and Herzegovina (BiH), but measures of democracy conceal an uneven subnational distribution of autocratization. So far there has been limited research on the drivers and constraints to subnational autocratization. This paper aims to contribute to the literature on power-sharing by exploring instances of illiberal politics enacted by parties in government at the subnational level in BiH. Evidence is gathered through semi-structured interviews and analysis of three specific cases of illiberal politics. We find that the political contest in BiH is purposefully contained within ethnic and subnational boundaries and constrained through several layers of institutionalized multilevel and ethnic checks and balances. The main drivers of subnational autocratization are opportunities that arise from the institutional framework established during early democratization and postwar structures that blend executive dominance with economic power and informal party networks, and occasionally from an individual actor’s perceptions of threat. Democratization in BiH will need to address subnational politics and deep-rooted power structures if it is to be successful.