汉族人群五个STR基因座的多态性调查

应用PCR及PAG电泳技术研究了PLA2A、vWA、CYP19、TH和LPL五个基因座的多态性，调查武汉地区汉族无关个体，获得汉族人群的频率分布。五个基因座基因型频率分布与Hardy-Weinberg平衡吻合良好、分别计算基因座的杂合度（H）、个人识别能力（Dp）、非父排除率（PE）和多态性信息总量（PIC）。为法医学应用提供了基础数据。     

用引物Y_3,Y_4和DNA扩增技术作血液(痕)和毛根性别鉴定的研究

作者用引物Y_3、Y_4和DNA聚合酶链式反应(PCR)作微量人类血液(痕)和毛根的性别鉴定。扩增的靶序列位于Y染色体DNA特异3.4kb重复序列中,扩增产物为460bp。检材用量为:新鲜血液0.5μl、血痕纱纤维1mm、毛根单个。20例保存4个月的血痕与2例保存6年半的血痕性别判定结果均正确,无性别记载的保存9～11年的3例血痕显现了清晰的460bpY特异DNA扩增带。15例保存20天的自然脱落毛根性别判定结果均正确。本法省略了检材处理中的酚-氯仿抽提DNA等纯化步骤,既简化了实验操作,又减少了检验过程中外源DNA的污染机会和样品DNA的损耗,使这一性别鉴定方法更符合法医学实践的需要。     

中国5个群体D20S85基因座的遗传多态性

目的 了解中国5个群体D20S85基因座的群体遗传学数据,比较它们之间的遗传学差异,探讨其在法医学应用中的意义。方法 分别收集5个群体622名无关个体的血样,Chelex-100快速抽提法或饱和酚/氯仿法抽提DNA;扩增后经PAGE垂直板电泳、银染,进行D20S85基因座分型。结果 在5个群体622名无关个体中,共检出9个等位基因,并首次在广东汉族和广西壮族群体中检出等位基因14;每个群体基因频率大于0.05的均为6个,D20S85*6为最常见等位基因。5个群体共观察到35种基因型,群体内基因型频率分布均符合Hardy-Weinberg氏平衡,各群体间基因型构成比无显著性差异。观察140次减数分裂未发现突变。各群体的期望杂合度为0.7720～0.7912;非父排除率,在三联体为0.7538～0.7594,二联体为0.3988～0.4297;个人识别率为0.9175～0,9272;多态信息含量为0.7442～0.7656。应用于亲子鉴定和个人识别案例,效果满意。结论 D20S85基因座是法医学应用价值较高的遗传标记系统。     

Thyreoglobulin and violent asphyxia

The concentration of thyreoglobulin (tg) was determined for death caused by hanging, strangulation by ligature, and throttling. Cases of sudden death (traumatic aortic rupture, penetrating wounds of the heart) were used for comparison. The mean values in cases of hanging (149.9±202.3 ng/ml), strangulation by ligature (193.1±173.3), manual strangulation (561.6±173.9) are distinguishable from violent acute deaths (23.3±27.6) and living healthy individuals (17.3±16.1). By means of statistical comparisons, significant differences were found between throttling and strangulation by ligature and between throttling and hanging (adjusted P<0.001). In connection with examination of the bodies high tg values can be regarded as a vital reaction in obstructive asphyxia.     

英汉颜色词的国俗语义对比与文化教学

词语所承载的意义不仅包含它的概念意义,还包含它的社会文化色彩和情感内涵。国俗语义是语义民族性的一种表现。英汉两种语言中颜色词语的国俗语义相同性很小。英汉颜色词语在社会属性、情感和心理方面存在差异,英汉颜色词语国俗语义缺项。     

社会稳定与发展状况的公众心态分析

近年来在公众的社会心态中 ,对社会稳定与发展的评价逐年提高 ,然而 ,由于改革的复杂性 ,以及社会各阶层利益的多样性 ,公众社会心态又存在着影响社会稳定的不确定因素。应采取适当措施 ,维护和发展群众的根本利益 ,保持社会的稳定与发展     

Commentary

Abstract

Women prisoners make up a small percentage of the prison population, yet they are the fastest growing population in prison, especially among women of color. Many of these women are typically young, poor, and uneducated mothers, and they often encounter an assortment of social and health problems while incarcerated. This commentary examines some of the issues that predispose women of color to commit crimes and the challenges they face in the New York State prison system.     

解读西南少数民族崇尚的色彩语言—"黑"

色彩语言是指人类社会中作为信息传递手段的色彩和意义结合的符号系统。西南不少民族崇尚黑色,以黑为美,以黑为贵,就在于色彩语言中的"黑"能传递出他们所企盼的信息。影响民族尚黑习俗形成的因素是复杂多样的,主要有三大因素:一是西南地区特有的自然环境和生产方式;二是原始宗教;三是历史上的文化接触与融合。但民族尚黑习俗的形成并不要求这三大因素必须同时起作用。     

Simplified Direct PCR Method for Reference Buccal Samples Using a Non-FTA Card by Omitting the Pretreatment Step

When using non-FTA cards in commercial multiplex STR kits for direct PCR, pretreatment steps with specific buffers are recommended. Here, we designed a rapid direct PCR method utilizing a non-FTA card, Oral Cell Sampling Kit, by omitting the pretreatment step involving Prep-n-Go™ Buffer, and it showed compatibility with the GlobalFiler™ Express PCR Amplification Kit, GlobalFiler™ PCR Amplification Kit, and PowerPlex^® Fusion system. To optimize the PCR conditions, we tested the method with different final PCR volumes and cycles. Finally, we conducted a performance test using 50 Korean buccal samples and confirmed the high performance of the method, detecting more than 90% of the samples with full profiles when using GlobalFiler™ PCR Amplification Kit and PowerPlex^® Fusion system at 29 cycles in a 10 μL final PCR volume. Thus, we report a simple direct PCR set-up to analyze reference samples collected using a non-FTA card manufactured in Korea.     

Listening to color: a set of propositions on pain as feminist aesthetic

This performative text asks whether it is possible to imagine an antisocial feminist aesthetic that does not invoke the wounded body. Its form – numbered propositions that engage with critical theory, literature, and memoir – riffs on that of Maggie Nelson’s Bluets, a key interlocutor for this question of aestheticizing embodied pain. How does Nelson’s genealogy of blue model a methodology for a “queer genealogy of femininity,” to borrow Jack Halberstam’s term? How does looking at color in this way offer an approach to narrating trauma, one that displaces the primacy of the visual? Turning to the work of Christina Crosby, Toni Morrison, Fred Moten, and José Esteban Muñoz, as well as drawing upon the author’s experience as a volunteer for a peer listening hotline, this essay considers the implications of “listening to color” for minoritarian knowledge formations and feminist praxis.