A mixed DNA profile controversy revisited

Semaan et al. (J Forensic Res, 2020, 11, 453) discuss a mock case “where eight different individuals [P₁ through P₈] could not be excluded in a mixed DNA analysis. Even though … expert DNA mixture analysis software was used.” Two of these are the true donors. The LRs reported are incorrect due to the incorrect entry of propositions into LRmix Studio. This forced the software to account for most of the alleles as drop-in, resulting in LRs 60–70 orders of magnitude larger than expected. P₁, P₂, P₄, P₅, and P₈ can be manually excluded using peak heights. This has relevance when using LRmix which does not use peak heights. We extend the work using the same two reference genotypes who were the true contributors as Semaan et al. (J Forensic Res, 2020, 11, 453). We simulate three two-donor mixtures with peak heights using these two genotypes and analyze using STRmix?. For the simulated 1:1 mixture, one of the non-donors’ LRs supported him being a contributor when no conditioning was used. When considered in combination with any other potential donors (i.e., with conditioning), this non-donor was correctly eliminated. For the 3:1 mixture, all results correctly supported that the non-donors were not contributors. The low-template 4:1 mixture LRs with no conditioning showed support for all eight profiles as donors. However, the results from pair-wise conditioning showed that only the two ground truth donors had LRs supporting that they were contributors to the mixture. We recommend the use of peak heights and conditioning profiles, as this allows better sensitivity and specificity even when the persons share many alleles.     

商标侵权惩罚性赔偿的制度构建

我国《商标法》2013年第三次修改时首次在知识产权领域引入惩罚性赔偿责任。实证分析表明,惩罚性赔偿在商标侵权诉讼中适用甚少且适用不规范的问题突出,未能充分发挥其遏制侵权行为的预期作用。究其原因,主要存在适用条件不明确、赔偿基数难确定、程序保障不充分等问题。为规范适用,充分发挥惩罚性赔偿的作用,需要从以下四个方面完善其制度构建:一是强化程序保障;二是明确适用条件;三是完善赔偿数额的计算;四是厘清惩罚性赔偿与法定赔偿的关系。     

工人阶级仍然是当代我国最先进的阶级

要回答中国工人阶级是否先进的问题,必须首先掌握先进性的衡量标准,并用其对中国工人阶级进行具体分析。     

Fundamental principles of the international protection system of intellectual property rights and the applications

Legal principle, which underlies the value of the legal system, is supposed to be the origin and basis of concrete legal rules. It has also resulted from abstracting and summarizing the value and spirit of these legal rules. In light of the universality and hierarchy of legal principle, the principles of the international protection system of intellectual property rights (IPR) can be divided into the following two types: one is the fundamental principles applied to the what, why and how a legal system shall be constructed, such as principles of sovereignty, equality and mutual benefit, joint development, and international cooperation, which also can be expressed as the principles of sovereignty, international coordination and cooperation, fairness and justice. The other type includes those existing in the legal system and capable of being applied directly, such as the principle of national treatment, principle of minimum standard, principle of independence (for industrial property right), principle of independent protection (for copyright), principle of compulsory implementing patent (for patent right) and doctrine of priority (for industrial property right), etc. In my opinion, the principles of the international protection system of IPR shall follow the latter model, indicating that they shall be provided and written in the international conventions on the grounds that they can be applied directly, and that they can be universally applied to the whole international protection system of IPR instead of exclusive application to one certain system. Hereupon, the author believes that principles concerning the international protection system of IPR should include the principles of national treatment, minimum protection standard and public interest.     

应用硅珠法提取陈旧骨骼DNA

骨骼相对于人体其他的组织脏器腐败降解慢,是进行高度腐败及白骨化样本DNA检验的唯一检材。但骨骼DNA的提取比较困难,本文运用传统的硅珠法提取骨骼DNA取得了很好的效果。1材料与方法1.1样本1~4年水浸泡、土埋的股骨、肱骨等样本,来自本实验室日常检案检材。1.2方法1.2.1骨骼DNA提取用水洗净骨骼表面,晾干。锯成小块,用锉打磨内外表面。去离子水、乙醇和5%bleach浸泡、晾干,磨成粉末。取骨粉5g;加入0.5mon/L EDTA(pH8.0)溶液,搅拌均匀置于摇床上脱钙12h,去离子水洗两遍,去上清,重复4次[1];沉渣中加入适量裂解液(6mon/L GuSCN,20mo…     

民事责任的两种规范模式

民事权利义务规范的设定有行为本位和资源本位两种模式,民事责任规范作为民事规范的一种也不例外。传统的学说将民事责任界定为义务的违反显然是行为本位视角的产物,实际上民事责任的产生与当事人的义务违反没有必然联系。民事责任是为保护民事权利或者法益,基于特定的法律事实而对一定的民事主体产生的不利后果。根据民事规范的设定模式,可以把民事责任规范设定模式分为“义务(行为)——责任”模式和“权利(资源)——责任”模式两种,其中前者是行为本位视角的产物,后者是资源本位视角的产物。     

试论司法鉴定技术标准体系建设

本文在分析司法鉴定技术标准的现状和司法公正对司法鉴定标准化需求的基础上,提出司法鉴定技术标准体系的总体构架以及标准体系建设的目标、内容及基本原则,尤其对司法鉴定技术标准的推行体系,包括管理体制、运行机制、实施体系、保障体系的建设进行了制度设计。     

环境税收制度的价值定位及改革方向

伴随着工业化进程所带来的严重的生态危机,人类不断探寻更为有效的保护环境的新举措。建立环境税收制度,将税收和环保结合起来,可以发挥税收在控制污染、调节资源配置方面的作用;应以可持续发展为核心,以生态利益为本位,以实现代际公平为主要目标;应改革传统排污收费制度、调整资源税和消费税、完善税收优惠政策和征管制度,从而构建我国新型的环境税收制度。     

DNA commission of the International Society of Forensic Genetics: Recommendations on the interpretation of mixtures

The DNA commission of the International Society of Forensic Genetics (ISFG) was convened at the 21st congress of the International Society for Forensic Genetics held between 13 and 17 September in the Azores, Portugal. The purpose of the group was to agree on guidelines to encourage best practice that can be universally applied to assist with mixture interpretation. In addition the commission was tasked to provide guidance on low copy number (LCN) reporting. Our discussions have highlighted a significant need for continuing education and research into this area. We have attempted to present a consensus from experts but to be practical we do not claim to have conveyed a clear vision in every respect in this difficult subject. For this reason, we propose to allow a period of time for feedback and reflection by the scientific community. Then the DNA commission will meet again to consider further recommendations.     

牛分枝杆菌esat-6和mpb70-mpb83基因DNA疫苗的免疫效果

用PCR技术和重叠延伸剪接技术获得牛分枝杆菌esat-6基因和mpb70-mpb83融合基因,连接真核表达载体pcDNA3.1(+),构建了重组质粒pCE6和pC70-83-E6。分4组免疫小鼠:pCE6组、pC70-83-E6组、pcDNA3.1(+)和PBS对照组,采用间接ELISA法检测免疫小鼠血清特异性抗体水平,MTT法检测免疫小鼠脾淋巴细胞增殖情况和IFN-γ分泌情况。结果表明,2重组质粒免疫组小鼠的血清抗体水平持续上升,而2对照组始终维持在较低水平,且pC70-83-E6组小鼠的抗体水平高于pCE6组。经PPD刺激后,pCE6组和pC70-83-E6组小鼠的SI值与2对照组均差异显著(P<0.05),2重组质粒免疫组间差异不显著(P>0.05);2重组质粒免疫小鼠脾细胞产生的IFN-γ均显著高于2对照组(P<0.05),且pC70-83-E6组明显高于其他3组(P<0.05)。证实本试验构建的2种牛分枝杆菌DNA疫苗可有效诱导实验动物产生体液免疫和细胞免疫应答。