排序方式: 共有3条查询结果,搜索用时 62 毫秒
1
1.
2.
夏雨 《贵州警官职业学院学报》2007,19(5):70-72
在两步消化法的基础上,采用DNA IQ^TM system提取精子DNA.通过磁性颗粒饱和性吸附.纯化模板DNA.稳定模板浓度,得到清晰完整的DNA分型.结合Biomek3000自动工作站.可有效减少人为因素的不确定性。提高检出率. 相似文献
3.
Hiron Poon M.Sc. ; Jim Elliott B.Sc. ; Jeff Modler M.Sc. ; Chantal Frégeau Ph.D. 《Journal of forensic sciences》2009,54(6):1278-1286
Abstract: Following implementation of our automated process incorporating the Promega DNA IQTM system as a DNA extraction method, a large number of blood-containing exhibits failed to produce DNA. These exhibits had been tested with the Hemastix® reagent strip, commonly used by police investigators and forensic laboratories as a screening test for blood. Some exhibits were even tainted green following transfer of the presumptive test reagents onto the samples. A series of experiments were carried out to examine the effect of the Hemastix® chemistries on the DNA IQTM system. Our results indicate that one or more chemicals imbedded in the Hemastix® reagent strip severely reduce the ability to recover DNA from any suspected stain using the DNA IQTM magnetic bead technology. The 3,3',5,5'-tetramethylbenzidine (TMB) used as the reporting dye appears to interact with the magnetic beads to prevent DNA recovery. Hydrogen peroxide does not seem to be involved. The Hemastix® chemistries do not interfere in any way with DNA extraction performed using phenol-chloroform. The incompatibility of the Hemastix® chemistries on the DNA IQTM system forced us to adopt an indirect approach using filter paper to carry out the presumptive test. 相似文献
1