排序方式: 共有32条查询结果,搜索用时 31 毫秒
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目的:研究脾虚状态时下丘脑-垂体-甲状腺轴功能的变化。方法:成年SD大鼠32只随机分为4组,每组8只,即正常组、脾虚组、治疗组、自复组。采用放射免疫分析法测定各组大鼠下丘脑、血清TRH,垂体、血清TSH及血清T3、T4、FT3、FT4、γT3的含量。结果:(1)脾虚组、自复组大鼠血清T3、T4、FT3、FT4以及下丘脑TRH、垂体TSH含量均较正常组、治疗组低,有极显差异(P<0.01);血清TRH、TSH含量均较正常组、治疗组高,有极显差异(P<0.01)。(2)治疗组大鼠上述指标与正常组无显性差异。结论:脾虚时下丘脑、垂体、甲状腺合成、分泌及调控功能低下,各级反馈不能使各层次激素水平恢复正常,部分反映了脾虚证的本质;健脾益气的四君子汤能修复下丘脑-垂体-甲状腺轴功能的损伤。 相似文献
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Investigating the Postmortem Molecular Biology of Cartilage and its Potential Forensic Applications 下载免费PDF全文
Shawna N. Bolton M.Sc. Michael P. Whitehead Ph.D. Jayesh Dudhia Ph.D. Timothy C. Baldwin Ph.D. Raul Sutton Ph.D. 《Journal of forensic sciences》2015,60(4):1061-1067
This study investigated the postmortem molecular changes that articular cartilage undergoes following burial. Fresh pig trotters were interred in 30‐cm‐deep graves at two distinct locations exhibiting dissimilar soil environments for up to 42 days. Extracts of the metacarpophalangeal (MCP) and metatarsophalangeal (MTP) joint cartilage from trotters disinterred weekly over 6 weeks were analyzed by Western blot against the monoclonal antibody 2‐B‐6 to assess aggrecan degradation. In both soil conditions, aggrecan degradation by‐products of decreasing molecular size and complexity were observed up to 21 days postmortem. Degradation products were undetected after this time and coincided with MCP/MTP joint exposure to the soil environment. These results show that cartilage proteoglycans undergo an ordered molecular breakdown, the analysis of which may have forensic applications. This model may prove useful for use as a human model and for forensic investigations concerning crimes against animals and the mortality of endangered species. 相似文献
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目的优化蛋白酶K的用量和消化时间,用于提取肋软骨DNA。方法 30份肋软骨样本各取大小为0.2cm×0.3cm×0.4cm的软骨块5份,分别编为A~E组。采用不同蛋白酶K用量和消化时间的Chelex100方法,提取5组各30份软骨块DNA,进行DNA定量、采用Sinofiler试剂盒扩增后进行电泳检测,采用非参数检验法比较检验成功率。结果 A~E组样本中采用加入2μL蛋白酶K并消化30min的D组方法成功率最高(96.7%),组间差异具有统计学意义。结论采用蛋白酶K-Chelex100方法,选择加入2μL蛋白酶K,消化30min可有效提高肋软骨DNA分型检验成功率。 相似文献
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Frank Wehner Heinz-Dieter Wehner Maria Christine Schieffer Jrg Subke 《Forensic Science International Supplement Series》2000,110(3):50
To improve the possibilities to delimit the time of death after longer laytime it was examined if this is possible by immunohistochemical detection of thyroglobulin. The results show that in our examination material the colloid and the follicular cells of the thyroid glands of up to 5-day-old corpses produce a positive immunoreaction towards thyroglobulin in all cases whereas none of the corpses older than 13 days show such a reaction. This means that in case of a negative immunoreaction the time of death can be assumed to lie more than 6 days before the autopsy. The fact that a negative immunoreaction occurrs consistently after 13 days leads to the conclusion that when thyroglobulin has been stained in a specimen, the death of the respective person must lie a maximum of 12 days earlier, whereby these time-limits may change in considerably different surrounding conditions. 相似文献
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Garvin HM 《Journal of forensic sciences》2008,53(5):1023-1027
As the role of forensic anthropologists expands to the medical examiner setting, their expertise is being applied beyond the traditional dry skeletal material. In such scenarios radiographic techniques can be applied when maceration is not appropriate. This study explores the use of radiographic analysis of laryngeal structures for age-at-death determination. Isolated human laryngeal structures (n = 104) from individuals between the ages of 15 and 89 were removed at autopsy and radiographically examined. The cricoid and individual regions of the thyroid cartilage were scored according to degree of ossification, and the relationship between age and degree of ossification statistically examined. A previously published study on age-determination from thyroid ossification by Cerny was assessed for accuracy. The results of the study indicate that although a consistent sequence in the ossification of laryngeal structures exists, variations in timing does not permit narrow age range estimates. Consequently the method presented by Cerny is inaccurate and should not be used in the forensic setting. 相似文献
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尸体甲状腺球蛋白降解及其与死亡时间的关系 总被引:3,自引:0,他引:3
目的探讨尸体甲状腺球蛋白降解程度与死亡时间(PM I)的关系。方法死后21d内尸体的甲状腺组织,经免疫组化(EPOS)法染色,观察甲状腺球蛋白的染色反应;对63例阳性染色的甲状腺组织(PM I≤5d)进行计算机图像分析,并对所得图像参数的数据进行统计学分析。结果PM I≤5d的甲状腺组织,甲状腺腺泡上皮细胞胞浆及甲状腺胶质中均显示了不同程度的免疫反应阳性;PM I超过12d,免疫反应阴性;经图像分析及统计学处理,其积分光密度、分布密度和目标面积与PM I之间的确定系数(R2)分别达0.9794,0.9732及0.9884。结论尸体甲状腺组织的甲状腺球蛋白降解程度随PM I不同而变化,二者呈现较强的相关性。 相似文献
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Rachel Wilson BA Clare McFadden PhD Samantha Rowbotham PhD 《Journal of forensic sciences》2023,68(3):731-742
The frequency and distribution of fractures are commonly utilized to assist in interpreting the manner of death. In cases of alleged suicide by hanging, however, the evidence base for the frequency and patterning of laryngohyoid and cervical vertebrae fractures resulting from such blunt force traumatic events is limited and so fractures cannot be reliably used to assist in interpreting the manner. Using meta-analytic techniques, this study aimed to estimate frequency and distribution of fractures in the context of relevant intrinsic and extrinsic variables. A systematic review of the literature identified 20 studies with relevant data (8523 cases of suicide by hanging). Meta-analyses identified the frequency and distribution of fractures present and how fracture frequency was affected by the subgroups of age, sex, completeness of suspension, ligature knot position and study design. Results indicated that fracture frequency was variable, there was no unique patterning, and high levels of heterogeneity were present in all variable sub-groups. Age was the only subgroup to show differences. Findings suggest that neck fracture frequency is inconsistent and cannot be predicted by the chosen variables. Subsequently, neck fractures in isolation should not be given weight in medico-legal interpretations of a hanging death as suicidal. 相似文献